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IP (Immunoprecipitation)
(ADA was immunoprecipitated using:Lane A:0.5 mg Jurkat Whole Cell Lysate4 uL anti-ADA rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-ADA rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 41 kDaObserved band size :43 kDa)
Rabbit anti-Human, Mouse HP1 Beta/CBX1 Polyclonal Antibody | anti-CBX1 antibody
Anti-HP1 Beta/CBX1 Antibody, Rabbit Polyclonal
Gene Names
CBX1; CBX; M31; MOD1; p25beta; HP1-BETA; HP1Hsbeta; HP1Hs-beta
Reactivity
Human, Mouse
Applications
Immunoprecipitation, Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
Protein A & Antigen Affinity
Synonyms
HP1 Beta/CBX1, Antibody; Anti-HP1 Beta/CBX1 Antibody, Rabbit Polyclonal; HP1 beta/CBX1 Antibody, Rabbit PAb, Antigen Affinity Purified; chromobox 1; Anti-CBX Antibody; Anti-HP1-BETA Antibody; Anti-HP1Hs-beta Antibody; Anti-HP1Hsbeta Antibody; Anti-M31 Antibody; Anti-MOD1 Antibody; Anti-p25beta Antibody; anti-CBX1 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human HP1 Beta/CBX1
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; PBS, pH7.0 with 0.03% Proclin300
Applicable Applications for anti-CBX1 antibody
IP (Immunoprecipitation), IF (Immunofluorescence), ICC (Immunocytochemistry), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthetic peptide corresponding to the center region of the Human HP1 beta/CBX1
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human HP1 beta/CBX1, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.
IP (Immunoprecipitation)
(ADA was immunoprecipitated using:Lane A:0.5 mg Jurkat Whole Cell Lysate4 uL anti-ADA rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-ADA rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 41 kDaObserved band size :43 kDa)
IP (Immunoprecipitation)
(ADA was immunoprecipitated using:Lane A:0.5 mg Jurkat Whole Cell Lysate4 uL anti-ADA rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-ADA rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 41 kDaObserved band size :43 kDa)
IF (Immunofluorescence)
(Immunofluorescence staining of CBX1 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human CBX1 polyclonal antibody (dilution ratio 1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Nucleus.)
IF (Immunofluorescence)
(Immunofluorescence staining of CBX1 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human CBX1 polyclonal antibody (dilution ratio 1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Nucleus.)
IHC (Immunohistochemisry)
(Immunochemical staining CBX1 in mouse testis with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)
IHC (Immunohistochemisry)
(Immunochemical staining CBX1 in mouse testis with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)
IHC (Immunohiostchemistry)
(Immunochemical staining CBX1 in human testis with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)
IHC (Immunohiostchemistry)
(Immunochemical staining CBX1 in human testis with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)
WB (Western Blot)
(Anti-CBX1 rabbit polyclonal antibody at 1:500 dilutionLane A: HeLa Whole Cell LysateLane B: MCF7 Whole Cell LysateLane C: A431 Whole Cell LysateLane D: 293T Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:21 kDa)
WB (Western Blot)
(Anti-CBX1 rabbit polyclonal antibody at 1:500 dilutionLane A: HeLa Whole Cell LysateLane B: MCF7 Whole Cell LysateLane C: A431 Whole Cell LysateLane D: 293T Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:21 kDa)
Related Product Information for anti-CBX1 antibody
Chromobox (CBX) family proteins are canonical components in polycomb repressive complexes 1 (PRC1), with epigenetic regulatory function and transcriptionally repressing target genes via chromatin modification. A plethora of studies have highlighted the function specifications among CBX family members in various cancer, including lung cancer, colon cancer and breast cancer. CBX1 exhibits oncogenic activities in hepatocellular carcinoma (HCC) and indicates poor outcomes. The expression of CBX1 was noticeably increased, at both mRNA and protein levels, in HCC tissues and cell lines, compared with the nontumorous ones. High CBX1 expression was significantly associated with larger tumor size, poor tumor differentiation and tumor vascular invasion. CBX1 functions as an oncogene and may serve as a potential prognostic biomarker in HCC.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
185
NCBI Official Full Name
Chromobox protein homolog 1
NCBI Official Synonym Full Names
chromobox homolog 1
NCBI Official Symbol
CBX1
NCBI Official Synonym Symbols
CBX; M31; MOD1; p25beta; HP1-BETA; HP1Hsbeta; HP1Hs-beta
NCBI Protein Information
chromobox protein homolog 1; HP1 beta homolog; modifier 1 protein; heterochromatin protein 1-beta; heterochromatin protein p25 beta; heterochromatin protein 1 homolog beta; chromobox homolog 1 (HP1 beta homolog Drosophila )
UniProt Protein Name
Chromobox protein homolog 1
UniProt Gene Name
CBX1
UniProt Synonym Gene Names
CBX; HP1 beta
UniProt Entry Name
CBX1_HUMAN
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Product Notes
The CBX1 cbx1 (Catalog #AAA254336) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-HP1 Beta/CBX1 Antibody, Rabbit Polyclonal reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's HP1 Beta/CBX1 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), IF (Immunofluorescence), ICC (Immunocytochemistry), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the CBX1 cbx1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HP1 Beta/CBX1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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