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IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded Prostate Cancer using HP1-gamma mouse mAb (1/200 dilution).Antigen retrieval was performed by pressure cooking in citrate buffer (pH 6.0).)
Mouse HP1-gamma Monoclonal Antibody | anti-HP1-gamma antibody
HP1-gamma Mouse mAb
Gene Names
CBX3; HECH; HP1-GAMMA; HP1Hs-gamma
Reactivity
Mouse, Human, Monkey, Hamster, Rat
Applications
Immunohistochemistry, Immunoprecipitation, Immunocytochemistry, Immunofluorescence, Western Blot
Purity
The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.
Synonyms
HP1-gamma, Antibody; HP1-gamma Mouse mAb; CBX 3; CBX3; CBX3_HUMAN; Chromobox homolog 3 (HP1 gamma homolog, Drosophila); Chromobox homolog 3; Chromobox protein homolog 3; GAMMA; HECH; Heterochromatin like protein 1; Heterochromatin protein 1 homolog gamma; Heterochromatin protein HP1 gamma; HP1 gamma; HP1 gamma homolog; HP1Hs gamma; Modifier 2 protein.; anti-HP1-gamma antibody
Host
Mouse
Reactivity
Mouse, Human, Monkey, Hamster, Rat
Clonality
Monoclonal
Specificity
This antibody detects endogenous levels of HP1-gamma and does not cross-react with related proteins.
Purity/Purification
The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.
Form/Format
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-HP1-gamma antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), ICC (Immunocytochemistry), IF (Immunofluorescence), WB (Western Blot)
Immunogen
Purified recombinant human HP1-gamma protein fragments expressed in E coli.
Modification
Unmodified/Total
Preparation and Storage
Store at -20 degree C/1 year
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded Prostate Cancer using HP1-gamma mouse mAb (1/200 dilution).Antigen retrieval was performed by pressure cooking in citrate buffer (pH 6.0).)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded Prostate Cancer using HP1-gamma mouse mAb (1/200 dilution).Antigen retrieval was performed by pressure cooking in citrate buffer (pH 6.0).)
ICC (Immunocytochemistry)
(Immunocytochemistry staining of HeLa cells fixed with 4% Paraformaldehyde and using anti-HP1-gamma mouse mAb (dilution 1:200).)
ICC (Immunocytochemistry)
(Immunocytochemistry staining of HeLa cells fixed with 4% Paraformaldehyde and using anti-HP1-gamma mouse mAb (dilution 1:200).)
ICC (Immunocytochemistry)
(Immunocytochemistry staining of HeLa cells fixed with -20? Methanol and using HP1-gamma (200137-5G10.dilution 1:200) mouse mAb (green) and Histone H3.1 (Phospho-Ser10)(310045.dilution 1:200) Rabbit pAb (red). DAPI was used to stain nucleus(blue).)
ICC (Immunocytochemistry)
(Immunocytochemistry staining of HeLa cells fixed with -20? Methanol and using HP1-gamma (200137-5G10.dilution 1:200) mouse mAb (green) and Histone H3.1 (Phospho-Ser10)(310045.dilution 1:200) Rabbit pAb (red). DAPI was used to stain nucleus(blue).)
IP (Immunoprecipitation)
(Immunoprecipitation analysis of Hela cell lysates using HP1-gamma mouse mAb.)
IP (Immunoprecipitation)
(Immunoprecipitation analysis of Hela cell lysates using HP1-gamma mouse mAb.)
WB (Western Blot)
(Western blot detection of HP1-gamma in Hela.3T3.C6.COS7 and CHO-K1 cell lysates using HP1-gamma mouse mAb (1:1000 diluted).Predicted band size:22KDa.Observed band size:22KDa.)
WB (Western Blot)
(Western blot detection of HP1-gamma in Hela.3T3.C6.COS7 and CHO-K1 cell lysates using HP1-gamma mouse mAb (1:1000 diluted).Predicted band size:22KDa.Observed band size:22KDa.)
Related Product Information for anti-HP1-gamma antibody
At the nuclear envelope, the nuclear lamina and heterochromatin are adjacent to the inner nuclear membrane.The protein encoded by this gene binds DNA and is a component of heterochromatin. This protein also can bind lamin B receptor, an integral membrane protein found in the inner nuclear membrane. The dual binding functions of the encoded protein may explain the association of heterochromatin with the inner nuclear membrane. This protein binds histone H3 tails methylated at Lys-9 sites. This protein is also recruited to sites ofultraviolet-induced DNA damage and double-strand breaks. Two transcript variants encoding the same protein but differing in the 5' UTR, have been found for this gene.[provided by RefSeq, Mar 2011]
Product Categories/Family for anti-HP1-gamma antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
20,811 Da
NCBI Official Full Name
chromobox protein homolog 3
NCBI Official Synonym Full Names
chromobox homolog 3
NCBI Official Symbol
CBX3
NCBI Official Synonym Symbols
HECH; HP1-GAMMA; HP1Hs-gamma
NCBI Protein Information
chromobox protein homolog 3; HP1 gamma homolog; modifier 2 protein; heterochromatin-like protein 1; heterochromatin protein HP1 gamma; heterochromatin protein 1 homolog gamma; chromobox homolog 3 (HP1 gamma homolog, Drosophila)
UniProt Protein Name
Chromobox protein homolog 3
UniProt Gene Name
CBX3
UniProt Synonym Gene Names
HP1 gamma
UniProt Entry Name
CBX3_HUMAN
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Product Notes
The HP1-gamma cbx3 (Catalog #AAA320298) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The HP1-gamma Mouse mAb reacts with Mouse, Human, Monkey, Hamster, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's HP1-gamma can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), ICC (Immunocytochemistry), IF (Immunofluorescence), WB (Western Blot). Researchers should empirically determine the suitability of the HP1-gamma cbx3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HP1-gamma, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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