Chicken anti-Human HSP27/HSBP1 Polyclonal Antibody | anti-HSP27/HSBP1 antibody

HSP27/HSBP1

Cat. Num. AAA76709

• Gene Names: HSBP1; NPC-A-13
• Reactivity: Human
• Applications: Immunofluorescence, Immunohistochemistry, Western Blot, Immunocytochemistry
• Synonyms: HSP27/HSBP1, Antibody; HSP27/HSBP1; anti-HSP27/HSBP1 antibody

• Host: Chicken
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Chicken IgY
• Form/Format: Liquid. Supplied as an aliquot of IgYpreparation plus 5mM NaN3

• Applicable Applications for anti-HSP27/HSBP1 antibody: IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot), ICC (Immunocytochemistry)
• Immunogen: Recombinant full length human HSP27 expressed in and purified from E coli
• Preparation and Storage: The antibody can be stored at 2 degree - 8 degree C for 12 months without detectable loss of activity. Avoidrepeated freeze-thaw cycles.

WB (Western Blot)

(Western blot analysis of whole cell lysates using chicken pAb to HSP27, AAA76709, dilution 1:2,000, in red. [1] protein standard (red), [2] NIH-3T3, [3] C6, [4] HeLa, [5] HEK293, [6] SH-SY5Y, and [7] COS-1 cells. Strong single band at 27kDa corresponds to the HSP27 protein strongly expressed in human cell lines.)

IF (Immunofluorescence)

(Immunofluorescent analysis of HeLa cells stained with chicken pAb to HSP27, AAA76709, dilution 1:1,000 in green, and costained with mouse mAb to HSP60 in red. Blue is DAPI staining of nuclear DNA. The CPCA-HSP27 antibody produces strong cytoplasmic staining, while the HSP60 antibody specifically labels mitochondria.)

Related Product Information for anti-HSP27/HSBP1 antibody

The heat shock proteins were discovered since they are heavily upregulated when cells are stressed by temperatures above the normal physiological range. They are expressed in unstressed cells also and have a normal function as chaperones, helping other proteins to fold correctly, but are required in much greater amounts if the cell or tissue is stressed by heat. Heat shock protein 27 (HSP27) is an abundant protein which functions as a chaperone but has many other functions. Upregulation of this protein is protective against neurodegenerative diseases at least in certain mouse models. Point mutations in the HSP27 gene are associated with two neurological diseases, Charcot-Marie-Tooth disease type 2F and distal hereditary motor neuropathy IIB. These diseases are associated with axonal loss apparently following defects in the transport of neurofilaments. HSP27 may bind cytoplamsic cytochrome c released from mitochondria which would otherwise normally activate the caspase apoptotic cascade. HSP27 is a major phosphoprotein of cells primarily under the influence of the p38/SAP kinase and JNK pathways. The central region of the HSP27 molecule corresponds to a crystallin domain, a compact module found in many chaperones and heat shock proteins. HSP27 is implicated in several other aspects of cell signaling and response to cancer and other diseases. The amino acid sequence of HSP27 is relatively poorly conserved across species boundaries, so there are 159 amino acids identities between human, cow, rat and mouse HSP27, out of 204-209 amino acids. This variability results in some antibodies being species specific while others work across many species.

CH22133 was made against full length recombinant HSP27 expressed in and purified from E coli. This antibody binds to an epitope on the human molecule missing from the rat and mouse homologue, likely variable peptides in the N and C terminal of human HSP27.

NCBI and Uniprot Product Information

• NCBI GI #: 4557647
• NCBI GeneID: 3281
• NCBI Accession #: NP_001528.1
• NCBI GenBank Nucleotide #: NM_001537.3
• UniProt Accession #: O75506; Q53XA8; Q7Z5Z3
• Molecular Weight: 8,544 Da
• NCBI Official Full Name: heat shock factor-binding protein 1
• NCBI Official Synonym Full Names: heat shock factor binding protein 1
• NCBI Official Symbol: HSBP1
• NCBI Official Synonym Symbols: NPC-A-13
• NCBI Protein Information: heat shock factor-binding protein 1; nasopharyngeal carcinoma-associated antigen 13
• UniProt Protein Name: Heat shock factor-binding protein 1
• UniProt Gene Name: HSBP1
• UniProt Synonym Gene Names: HSF1BP; NPC-A-13
• UniProt Entry Name: HSBP1_HUMAN