Mouse anti-Human HSP60 (Heat Shock Protein 60) Monoclonal Antibody | anti-HSPD1 antibody

HSP60 (Heat Shock Protein 60) (Mitochondrial Marker)

Cat. Num. AAA215454

• Gene Names: HSPD1; HLD4; CPN60; GROEL; HSP60; HSP65; SPG13; HSP-60; HuCHA60
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry, Functional Assay
• Purity: Purified Ab with BSA and Azide at 200ug/ml or Purified Ab with BSA and Azide at 200ug/ml or Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Synonyms: HSP60 (Heat Shock Protein 60), Antibody; HSP60 (Heat Shock Protein 60) (Mitochondrial Marker); 60kDa chaperonin; 60kDa heat shock protein mitochondrial; Chaperonin; 60-KD (CPN60); GROEL; HLD4; HSP65; HSPD1; HuCHA60; Mitochondrial matrix protein P1; P60 lymphocyte protein; Short heat shock protein 60 Hsp60s1; Spastic paraplegia 13 (SPG13); anti-HSPD1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone Number: AE-1
• Specificity: Recognizes a 60kDa protein, identified as the heat shock protein 60 (hsp60). A wide variety of environmental and pathophysiological stressful conditions trigger the synthesis of a family of proteins known as heat shock proteins (hsps). Hsp60 is a potential antigen in a number of autoimmune diseases. In human arthritis and in experimentally induced arthritis in animals, disease development coincides with the development of immune reactivity directed against not only bacterial hsp60, but also against its mammalian homolog.
• Purity/Purification: Purified Ab with BSA and Azide at 200ug/ml or Purified Ab with BSA and Azide at 200ug/ml or Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0mg/ml.
• Sequence Length: 573

• Applicable Applications for anti-HSPD1 antibody: WB (Western Blot), IHC (Immunohistochemistry), IF (Immunofluorescence), FCM/FACS (Flow Cytometry)
• Immunogen: Recombinant Human HSPD1 protein
• Cellular Localization: Mitochondrial
• Hu-chromosome Location: 2q33.1
• Positive Control: HeLa or HepG2 cells. Breast carcinoma.
• Preparation and Storage: Antibody with azide: Store at 2 to 8 degree C.; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

IF (Immunofluorescence)

(Immunofluorescent staining of PFA-fixed HeLa cells. HSP60 Monoclonal Antibody (AE-1) (CF488). Nuclei stained with RedDot (red).)

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with HSP60 Mouse Monoclonal Antibody (AE-1).)

References

Schlesinger, M.J., et al. 1982. Heat Shock: from Bacteria to Man. Cold Spring Harbor, N.Y.: Cold Spring Harbor Laboratory.

NCBI and Uniprot Product Information

• NCBI GI #: 31542947
• NCBI GeneID: 3329
• NCBI Accession #: NP_002147.2
• NCBI GenBank Nucleotide #: NP_002147.2
• UniProt Accession #: P10809
• Molecular Weight: 60kDa
• NCBI Official Full Name: 60 kDa heat shock protein, mitochondrial
• NCBI Official Synonym Full Names: heat shock protein family D (Hsp60) member 1
• NCBI Official Symbol: HSPD1
• NCBI Official Synonym Symbols: HLD4; CPN60; GROEL; HSP60; HSP65; SPG13; HSP-60; HuCHA60
• NCBI Protein Information: 60 kDa heat shock protein, mitochondrial
• UniProt Protein Name: 60 kDa heat shock protein, mitochondrial
• UniProt Gene Name: HSPD1
• UniProt Synonym Gene Names: HSP60; CPN60; HSP-60; Hsp60
• UniProt Entry Name: CH60_HUMAN