IF (Immunofluorescence)
(Immunofluorescence staining of Hsp90 alpha in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human Hsp90 alpha polyclonal antibody (dilution ratio 1:100) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.)
Rabbit anti-Human Hsp90 alpha Polyclonal Antibody | anti-HSP90AA1 antibody
Anti-Hsp90 alpha Antibody, Rabbit Polyclonal
Gene Names
HSP90AA1; HSPN; LAP2; HSP86; HSPC1; HSPCA; Hsp89; Hsp90; HSP89A; HSP90A; HSP90N; HSPCAL1; HSPCAL4; FLJ31884
Reactivity
Human
Applications
Immunofluorescence, Immunocytochemistry, ELISA, Western Blot
Purity
Protein A & Antigen Affinity
Synonyms
Hsp90 alpha, Antibody; Anti-Hsp90 alpha Antibody, Rabbit Polyclonal; Hsp90 alpha Antibody, Rabbit PAb, Antigen Affinity Purified; Anti-EL52 Antibody; Anti-HSP86 Antibody; Anti-Hsp89 Antibody; Anti-HSP89A Antibody; Anti-Hsp90 Antibody; Anti-HSP90A Antibody; Anti-HSP90N Antibody; Anti-HSPC1 Antibody; Anti-HSPCA Antibody; Anti-HSPCAL1 Antibody; Anti-HSPCAL4 Antibody; Anti-HSPN Antibody; Anti-LAP-2 Antibody; Anti-LAP2 Antibody; heat shock protein 90kDa alpha (cytosolic), class A member 1; anti-HSP90AA1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human Hsp90 alpha
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-HSP90AA1 antibody
IF (Immunofluorescence), ICC (Immunocytochemistry), ELISA, WB (Western Blot)
Conjugation
Unconjugated
Immunogen
Recombinant Human Hsp90 alpha Protein;
Preparation
Produced in rabbits immunized with purified, recombinant Human Hsp90 alpha (rh Hsp90 alpha;NP_005339.3; Glu535-Asp732). Hsp90 alpha specific IgG was purified by Human Hsp90 alpha affinity chromatography.
Preparation and Storage
This antibody can be stored at 2 degree C-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
IF (Immunofluorescence)
(Immunofluorescence staining of Hsp90 alpha in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human Hsp90 alpha polyclonal antibody (dilution ratio 1:100) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.)
IF (Immunofluorescence)
(Immunofluorescence staining of Hsp90 alpha in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human Hsp90 alpha polyclonal antibody (dilution ratio 1:100) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.)
WB (Western Blot)
(Anti-HSP90 rabbit polyclonal antibody at 1:500 dilution Lane A: HeLa Whole Cell Lysate Lane B: HL-60 Whole Cell Lysate Lane C: HCT116 Whole Cell Lysate Lane D: MCF-7 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:85 kDa Observed band size:95 kDa)
WB (Western Blot)
(Anti-HSP90 rabbit polyclonal antibody at 1:500 dilution Lane A: HeLa Whole Cell Lysate Lane B: HL-60 Whole Cell Lysate Lane C: HCT116 Whole Cell Lysate Lane D: MCF-7 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:85 kDa Observed band size:95 kDa)
Related Product Information for anti-HSP90AA1 antibody
Heat shock protein 90 (90 kDa heat-shock protein, HSP90) is a molecular chaperone involved in the trafficking of proteins in the cell. It is a remarkably versatile protein involved in the stress response and normal homoeostatic control mechanisms. HSP90 interacts with 'client proteins', including protein kinases, transcription factors, and others, and either facilitates their stabilization and activation or directs them for proteasomal degradation. By this means, HSP90 displays a multifaceted ability to influence signal transduction, chromatin remodeling and epigenetic regulation, development, and morphological evolution. HSP90 operates as a dimer in a conformational cycle driven by ATP binding and hydrolysis at the N-terminus. Disruption of HSP90 leads to client protein degradation and often cell death. Under stressful conditions, HSP90 stabilizes its client proteins and protects the cell against cellular stressors such as in cancer cells. Especially, several oncoproteins act as HSP90 client proteins and tumor cells require higher HSP90 activity than normal cells to maintain their malignancy. For this reason, Hsp90 has emerged as a promising target for anti-cancer drug development.
Product Categories/Family for anti-HSP90AA1 antibody
References
Pearl LH, et al. (2008) The Hsp90 molecular chaperone: an open and shut case for treatment. Biochem J. 410(3): 439-53.Hahn JS. (2009) The Hsp90 chaperone machinery: from structure to drug development. BMB Rep. 42(10): 623-30.Holzbeierlein JM, et al. (2010) Hsp90: a drug target. Curr Oncol Rep. 12(2): 95-101.Trepel J, et al. (2010) Targeting the dynamic HSP90 complex in cancer. Nat Rev Cancer. 10(8): 537-49.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
84,660 Da
NCBI Official Full Name
heat shock protein HSP 90-alpha isoform 2
NCBI Official Synonym Full Names
heat shock protein 90kDa alpha (cytosolic), class A member 1
NCBI Official Symbol
HSP90AA1
NCBI Official Synonym Symbols
HSPN; LAP2; HSP86; HSPC1; HSPCA; Hsp89; Hsp90; HSP89A; HSP90A; HSP90N; HSPCAL1; HSPCAL4; FLJ31884
NCBI Protein Information
heat shock protein HSP 90-alpha; HSP 86; heat shock 86 kDa; heat shock 90kD protein 1, alpha; heat shock 90kDa protein 1, alpha; renal carcinoma antigen NY-REN-38; heat shock 90kD protein, alpha-like 4; heat shock 90kD protein 1, alpha-like 4
UniProt Protein Name
Heat shock protein HSP 90-alpha
UniProt Gene Name
HSP90AA1
UniProt Synonym Gene Names
HSP90A; HSPC1; HSPCA; HSP 86; HSP86
UniProt Entry Name
HS90A_HUMAN
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Product Notes
The HSP90AA1 hsp90aa1 (Catalog #AAA258523) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Hsp90 alpha Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Hsp90 alpha can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), ICC (Immunocytochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the HSP90AA1 hsp90aa1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Hsp90 alpha, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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