Rabbit anti-Human IGF1 Polyclonal Antibody | anti-IGF1 antibody

IGF1 Antibody (C-term)

Cat. Num. AAA289206

• Gene Names: IGF1; IGFI; IGF-I; IGF1A
• Reactivity: Human
• Applications: Immunohistochemistry, Flow Cytometry, Functional Assay, Immunofluorescence, ELISA, Western Blot
• Purity: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Synonyms: IGF1, Antibody; IGF1 Antibody (C-term); IGF1; IBP1; Insulin-like growth factor I; Mechano growth factor; Somatomedin-C; anti-IGF1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: This IGF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 115-144 amino acids from the C-terminal region of human IGF1.
• Purity/Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Sequence Positions: 115-144
• Sequence Length: 153

• Applicable Applications for anti-IGF1 antibody: IHC (Immunohistochemistry), FCM/FACS (Flow Cytometry), IF (Immunofluorescence), ELISA, WB (Western Blot)
• Preparation and Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

IHC (Immunohistochemistry)

(IGF1 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of IGF1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

IHC (Immunohistochemistry)

(IGF1 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of IGF1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

FCM/FACS (Flow Cytometry)

(IGF1 Antibody (C-term) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

IF (Immunofluorescence)

(Confocal immunofluorescent analysis of IGF1 Antibody (C-term) with A549 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)

WB (Western Blot)

(IGF1 Antibody (C-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the IGF1 antibody detected the IGF1 protein (arrow).)

Related Product Information for anti-IGF1 antibody

The protein encoded by this gene is similar to insulin in function and structure and is a member of a family of proteins involved in mediating growth and development. The encoded protein is processed from a precursor, bound by a specific receptor, and secreted. Defects in this gene are a cause of insulin-like growth factor I deficiency. Several transcript variants encoding different isoforms have been found for this gene.

References

Li, M., et al. J. Biol. Chem. 285(40):30480-30488(2010)
Canzian, F., et al. Hum. Mol. Genet. 19(19):3873-3884(2010)
Romero, R., et al. Am. J. Obstet. Gynecol. 203 (4), 361 (2010) :
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Szczesny, G., et al. Arch Orthop Trauma Surg (2010) In press :

NCBI and Uniprot Product Information

• NCBI GI #: 11024682
• NCBI GeneID: 3479
• NCBI Accession #: NP_000609.1; NP_001104754.1; NP_001104755.1
• NCBI GenBank Nucleotide #: NM_000618.3
• UniProt Accession #: P05019; P01343; Q14620; B2RWM7; E9PD02
• NCBI Official Full Name: insulin-like growth factor I isoform 4 preproprotein
• NCBI Official Synonym Full Names: insulin-like growth factor 1 (somatomedin C)
• NCBI Official Symbol: IGF1
• NCBI Official Synonym Symbols: IGFI; IGF-I; IGF1A
• NCBI Protein Information: insulin-like growth factor I
• UniProt Protein Name: Insulin-like growth factor I
• UniProt Gene Name: IGF1
• UniProt Synonym Gene Names: IBP1; IGF-I; MGF
• UniProt Entry Name: IGF1_HUMAN