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FCM/FACS (Flow Cytometry)
(IGF2 Antibody (Center R54) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit anti-Human, mouse IGF2 Polyclonal Antibody | anti-IGF2 antibody
IGF2 Antibody (Center R54)
Gene Names
IGF2; IGF-II; PP9974; C11orf43
Reactivity
Human, mouse
Applications
Immunohistochemistry, Western Blot, ELISA, Flow Cytometry, Functional Assay
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
IGF2, Antibody; IGF2 Antibody (Center R54); Insulin-like growth factor II; IGF-II; Somatomedin-A; Insulin-like growth factor II Ala-25 Del; Preptin; IGF2; anti-IGF2 antibody
Host
Rabbit
Reactivity
Human, mouse
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This IGF2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 39-68 amino acids from the Central region of human IGF2.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
39-68
Sequence Length
236
Applicable Applications for anti-IGF2 antibody
IHC (Immunohistochemistry), WB (Western Blot), ELISA, FCM/FACS (Flow Cytometry)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(IGF2 Antibody (Center R54) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(IGF2 Antibody (Center R54) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
WB (Western Blot)
(IGF2 Antibody (Center R54) western blot analysis in mouse cerebellum tissue lysates (35ug/lane).This demonstrates the IGF2 antibody detected the IGF2 protein (arrow).)
WB (Western Blot)
(IGF2 Antibody (Center R54) western blot analysis in mouse cerebellum tissue lysates (35ug/lane).This demonstrates the IGF2 antibody detected the IGF2 protein (arrow).)
IHC (Immunohiostchemistry)
(IGF2 Antibody (Center R54) immunohistochemistry analysis in formalin fixed and paraffin embedded human placenta tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of IGF2 Antibody (Center R54) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(IGF2 Antibody (Center R54) immunohistochemistry analysis in formalin fixed and paraffin embedded human placenta tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of IGF2 Antibody (Center R54) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot)
(IGF2 Antibody (Center R54) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the IGF2 antibody detected the IGF2 protein (arrow).)
WB (Western Blot)
(IGF2 Antibody (Center R54) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the IGF2 antibody detected the IGF2 protein (arrow).)
Related Product Information for anti-IGF2 antibody
This gene encodes a member of the insulin family of
polypeptide growth factors, which are involved in development and
growth. It is an imprinted gene, expressed only from the paternal
allele, and epigenetic changes at this locus are associated with
Wilms tumour, Beckwith-Wiedemann syndrome, rhabdomyosarcoma, and
Silver-Russell syndrome. A read-through INS-IGF2 gene exists, whose
5' region overlaps the INS gene and the 3' region overlaps this
gene. Alternatively spliced transcript variants encoding different
isoforms have been found for this gene.
polypeptide growth factors, which are involved in development and
growth. It is an imprinted gene, expressed only from the paternal
allele, and epigenetic changes at this locus are associated with
Wilms tumour, Beckwith-Wiedemann syndrome, rhabdomyosarcoma, and
Silver-Russell syndrome. A read-through INS-IGF2 gene exists, whose
5' region overlaps the INS gene and the 3' region overlaps this
gene. Alternatively spliced transcript variants encoding different
isoforms have been found for this gene.
Product Categories/Family for anti-IGF2 antibody
References
Adkins, R.M., et al. Pediatr. Res. 68(5):429-434(2010)
Romero, R., et al. Am. J. Obstet. Gynecol. 203 (4), 361 (2010) :
Li, J., et al. Mol. Biol. Rep. (2010) In press :
Hsieh, Y.Y., et al. Anticancer Res. 30(6):2203-2208(2010)
Turan, N., et al. PLoS Genet. 6 (7), E1001033 (2010) :
Romero, R., et al. Am. J. Obstet. Gynecol. 203 (4), 361 (2010) :
Li, J., et al. Mol. Biol. Rep. (2010) In press :
Hsieh, Y.Y., et al. Anticancer Res. 30(6):2203-2208(2010)
Turan, N., et al. PLoS Genet. 6 (7), E1001033 (2010) :
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
20140
NCBI Official Full Name
insulin-like growth factor II isoform 1 preproprotein
NCBI Official Synonym Full Names
insulin-like growth factor 2
NCBI Official Symbol
IGF2
NCBI Official Synonym Symbols
IGF-II; PP9974; C11orf43
NCBI Protein Information
insulin-like growth factor II
UniProt Protein Name
Insulin-like growth factor II
UniProt Gene Name
IGF2
UniProt Synonym Gene Names
IGF-II
UniProt Entry Name
IGF2_HUMAN
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Product Notes
The IGF2 igf2 (Catalog #AAA289663) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 39-68. The IGF2 Antibody (Center R54) reacts with Human, mouse and may cross-react with other species as described in the data sheet. AAA Biotech's IGF2 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot), ELISA, FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the IGF2 igf2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IGF2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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