FCM/FACS (Flow Cytometry)
(Overlay histogram showing A549 cells stained with AAA287458 (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AAA287458, 1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(1583138) at 1/200 dilution for 40 min at 37°C. Isotype control antibody (blue line) was rabbit IgG1 (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.)
Rabbit IGFBP4 Polyclonal Antibody | anti-IGFBP4 antibody
IGFBP4 Antibody (N-term)
Gene Names
IGFBP4; BP-4; IBP4; IGFBP-4; HT29-IGFBP
Reactivity
Human, Mouse, Rat (Predicted Reactivity: Bovine, Pig)
Applications
Flow Cytometry, Functional Assay, ELISA, Western Blot
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Synonyms
IGFBP4, Antibody; IGFBP4 Antibody (N-term); Insulin-like growth factor-binding protein 4; IBP-4; IGF-binding protein 4; IGFBP-4; IGFBP4; IBP4; anti-IGFBP4 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat (Predicted Reactivity: Bovine, Pig)
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
This IGFBP4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 66-92 amino acids from the N-terminal region of human IGFBP4.
Purity/Purification
This antibody is purified through a protein A column, followed by peptide affinity purification.
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Sequence Positions
66-92
Sequence Length
258
Applicable Applications for anti-IGFBP4 antibody
FCM/FACS (Flow Cytometry), ELISA, WB (Western Blot)
Function
IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.
Cellular Location
Secreted.
Preparation and Storage
Store at -20°C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(Overlay histogram showing A549 cells stained with AAA287458 (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AAA287458, 1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(1583138) at 1/200 dilution for 40 min at 37°C. Isotype control antibody (blue line) was rabbit IgG1 (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.)
FCM/FACS (Flow Cytometry)
(Overlay histogram showing A549 cells stained with AAA287458 (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AAA287458, 1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(1583138) at 1/200 dilution for 40 min at 37°C. Isotype control antibody (blue line) was rabbit IgG1 (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.)
WB (Western Blot)
(Anti-IGFBP4 Antibody (N-term) at 1:2000 dilution + Rat ovary lysate Lysates/proteins at 20 ug per lane.Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 28 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(Anti-IGFBP4 Antibody (N-term) at 1:2000 dilution + Rat ovary lysate Lysates/proteins at 20 ug per lane.Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 28 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(All lanes : Anti-IGFBP4 Antibody (N-term) at 1:2000 dilutionLane 1: A549 whole cell lysateLane 2: Mouse ovary lysateLane 3: Rat ovary lysate Lysates/proteins at 20 ug per lane.Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 28 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(All lanes : Anti-IGFBP4 Antibody (N-term) at 1:2000 dilutionLane 1: A549 whole cell lysateLane 2: Mouse ovary lysateLane 3: Rat ovary lysate Lysates/proteins at 20 ug per lane.Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 28 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Related Product Information for anti-IGFBP4 antibody
IGFBP4 has an IGFBP domain and a thyroglobulin type-I domain. The protein binds both insulin-like growth factors (IGFs) I and II and circulates in the plasma in both glycosylated and non-glycosylated forms. Binding of this protein prolongs the half-life of the IGFs and alters their interaction with cell surface receptors.
Product Categories/Family for anti-IGFBP4 antibody
References
Durai,R., et.al., Colorectal Dis 9 (7), 625-631 (2007)
Laursen,L.S., et.al., Mol. Endocrinol. 21 (5), 1246-1257 (2007)
Laursen,L.S., et.al., Mol. Endocrinol. 21 (5), 1246-1257 (2007)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
insulin-like growth factor-binding protein 4
NCBI Official Synonym Full Names
insulin-like growth factor binding protein 4
NCBI Official Symbol
IGFBP4
NCBI Official Synonym Symbols
BP-4; IBP4; IGFBP-4; HT29-IGFBP
NCBI Protein Information
insulin-like growth factor-binding protein 4
UniProt Protein Name
Insulin-like growth factor-binding protein 4
UniProt Gene Name
IGFBP4
UniProt Synonym Gene Names
IBP4; IBP-4; IGF-binding protein 4; IGFBP-4
UniProt Entry Name
IBP4_HUMAN
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Product Notes
The IGFBP4 igfbp4 (Catalog #AAA287458) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 66-92. The IGFBP4 Antibody (N-term) reacts with Human, Mouse, Rat (Predicted Reactivity: Bovine, Pig) and may cross-react with other species as described in the data sheet. AAA Biotech's IGFBP4 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the IGFBP4 igfbp4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IGFBP4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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