Rabbit anti-Human IGHM Polyclonal Antibody | anti-IGHM antibody

IGHM Antibody

Cat. Num. AAA234818

• Gene Names: IGHM; MU; VH; AGM1
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: >95%, Protein G purified
• Synonyms: IGHM, Antibody; IGHM Antibody; Immunoglobulin heavy constant mu; s; Ig mu chain C region; Ig mu chain C region GAL1; IGHM2 Publications; anti-IGHM antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >95%, Protein G purified
• Form/Format: Liquid
• Sequence Length: 453

• Applicable Applications for anti-IGHM antibody: IHC (Immunohistochemistry), WB (Western Blot), ELISA
• Immunogen: Recombinant Human Immunoglobulin heavy constant mu protein (45-178AA)
• Conjugation: Non-conjugated
• Buffer: Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
• Preparation and Storage: Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.

IHC (Immunohiostchemistry)

(IHC image of AAA234818 diluted at 1:600 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)

WB (Western Blot)

(Western BlotPositive WB detected in: SH-SY5Y whole cell lysate, MCF-7 whole cell lysate, U87 whole cell lysateAll lanes: IGHM antibody at 3.4ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 50, 52 KDaObserved band size: 50 KDa)

Related Product Information for anti-IGHM antibody

Constant region of immunoglobulin heavy chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens (PubMed:22158414, PubMed:20176268). The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen (PubMed:17576170, PubMed:20176268). IgM antibodies play an important role in primary defense mechanisms. They have been shown to be involved in early recognition of external invaders like bacteria and viruses, cellular waste and modified self, as well as in recognition and elimination of precancerous and cancerous lesions. The membrane-bound form is found in the majority of normal B-cells alongside with IgD. Membrane-bound IgM induces the phosphorylation of CD79A and CD79B by the Src family of protein tyrosine kinases. It may cause death of cells by apoptosis. It is also found in soluble form, which represents about 30% of the total serum immunoglobulins where it is found almost exclusively as a homopentamer. After the antigen binds to the B-cell receptor, the secreted form is secreted in large amounts (PubMed:3137579, PubMed:16895553).

Product Categories/Family for anti-IGHM antibody

Immunology

NCBI and Uniprot Product Information

• NCBI GI #: 1160421986
• NCBI GeneID: 3507
• UniProt Accession #: P01871; P04220; P20769; A0A075B6N9; A0A0G2JQL4
• Molecular Weight: 51,924 Da
• NCBI Official Full Name: Immunoglobulin heavy constant mu
• NCBI Official Synonym Full Names: immunoglobulin heavy constant mu
• NCBI Official Symbol: IGHM
• NCBI Official Synonym Symbols: MU; VH; AGM1
• UniProt Protein Name: Immunoglobulin heavy constant mu
• UniProt Gene Name: IGHM