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FCM/FACS (Flow Cytometry)
(IL1RN Antibody (C-term) (Cat. # AAA289486) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit anti-Human IL1RN Polyclonal Antibody | anti-IL1RN antibody
IL1RN Antibody (C-term)
Gene Names
IL1RN; DIRA; IRAP; IL1F3; IL1RA; MVCD4; IL-1RN; IL-1ra; IL-1ra3; ICIL-1RA
Reactivity
Human
Applications
ELISA, Western Blot, Immunofluorescence, Flow Cytometry, Functional Assay
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
IL1RN, Antibody; IL1RN Antibody (C-term); Interleukin-1 receptor antagonist protein; IL-1RN; IL-1ra; IRAP; ICIL-1RA; IL1 inhibitor; Anakinra; IL1RN; IL1F3; IL1RA; anti-IL1RN antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This IL1RN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 147-177 amino acids from the C-terminal region of human IL1RN.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Sequence Positions
147-177
Sequence Length
143
Applicable Applications for anti-IL1RN antibody
ELISA, WB (Western Blot), IF (Immunofluorescence), FCM/FACS (Flow Cytometry)
Function
Anti-inflammatory antagonist of interleukin-1 family of proinflammatory cytokines such as interleukin-1beta/IL1B and interleukin-1alpha/IL1A. Protects from immune dysregulation and uncontrolled systemic inflammation triggered by IL1 for a range of innate stimulatory agents such as pathogens.
Cellular Location
[Isoform 1]: Secreted [Isoform 3]: Cytoplasm.
Tissue Location
The intracellular form of IL1RN is predominantly expressed in epithelial cells
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(IL1RN Antibody (C-term) (Cat. # AAA289486) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(IL1RN Antibody (C-term) (Cat. # AAA289486) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
WB (Western Blot)
(IL1RN Antibody (C-term) (Cat.# AAA289486) western blot analysis in 293,Hela cell line lysates (35ug/lane).This demonstrates the IL1RN antibody detected the IL1RN protein (arrow).)
WB (Western Blot)
(IL1RN Antibody (C-term) (Cat.# AAA289486) western blot analysis in 293,Hela cell line lysates (35ug/lane).This demonstrates the IL1RN antibody detected the IL1RN protein (arrow).)
WB (Western Blot)
(Anti-IL1RN Antibody (C-term) at 1:4000 dilution + 293 whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 20 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(Anti-IL1RN Antibody (C-term) at 1:4000 dilution + 293 whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 20 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of IL1RN Antibody (C-term)(Cat# AAA289486) with WiDr cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of IL1RN Antibody (C-term)(Cat# AAA289486) with WiDr cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
Related Product Information for anti-IL1RN antibody
The protein is a member of the interleukin 1 cytokine family. This protein inhibits the activities of interleukin 1, alpha (IL1A) and interleukin 1, beta (IL1B), and modulates a variety of interleukin 1 related immune and inflammatory responses. A polymorphism of its gene is reported to be associated with increased risk of osteoporotic fractures and gastric cancer.
Product Categories/Family for anti-IL1RN antibody
References
Kumar,S., Biochem. Biophys. Res. Commun. 387 (3), 456-460 (2009) Rechcinski,T., Kardiol Pol 67 (6), 601-610 (2009)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
20055
NCBI Official Full Name
interleukin-1 receptor antagonist protein isoform 3
NCBI Official Synonym Full Names
interleukin 1 receptor antagonist
NCBI Official Symbol
IL1RN
NCBI Official Synonym Symbols
DIRA; IRAP; IL1F3; IL1RA; MVCD4; IL-1RN; IL-1ra; IL-1ra3; ICIL-1RA
NCBI Protein Information
interleukin-1 receptor antagonist protein
UniProt Protein Name
Interleukin-1 receptor antagonist protein
UniProt Gene Name
IL1RN
UniProt Synonym Gene Names
IL1F3; IL1RA; IL-1RN; IL-1ra; IRAP
UniProt Entry Name
IL1RA_HUMAN
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Product Notes
The IL1RN il1rn (Catalog #AAA289486) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 147-177. The IL1RN Antibody (C-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's IL1RN can be used in a range of immunoassay formats including, but not limited to, ELISA, WB (Western Blot), IF (Immunofluorescence), FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the IL1RN il1rn for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IL1RN, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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