WB (Western Blot)
(Western blot analysis of lysates from 293T, A431 cell line (from left to right), using IL4R Antibody (C-term). AAA284044 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)
Rabbit anti-Human IL4R Polyclonal Antibody | anti-IL4R antibody
IL4R Antibody (C-term)
Gene Names
IL4R; CD124; IL4RA; IL-4RA
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, ELISA, Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
IL4R, Antibody; IL4R Antibody (C-term); Interleukin-4 receptor subunit alpha; IL-4 receptor subunit alpha; IL-4R subunit alpha; IL-4R-alpha; IL-4RA; CD124; Soluble interleukin-4 receptor subunit alpha; Soluble IL-4 receptor subunit alpha; Soluble IL-4R-alpha; sIL4Ralpha/prot; IL-4-binding protein; IL4-BP; IL4R; IL4RA; anti-IL4R antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This IL4R antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 677-691 amino acids from the C-terminal region of human IL4R.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Sequence Length
825
Applicable Applications for anti-IL4R antibody
FCM/FACS (Flow Cytometry), ELISA, WB (Western Blot)
Function
Receptor for both interleukin 4 andinterleukin 13. Couples to the JAK1/2/3-STAT6pathway. The IL4 response is involved inpromoting Th2 differentiation. The IL4/IL13responses are involved in regulating IgEproduction and, chemokine and mucusproduction at sites of allergic inflammation. Incertain cell types, can signal throughactivation of insulin receptor substrates,IRS1/IRS2.
Cell Location
Cell membrane; Single-pass type I membraneprotein
Tissue Location
Isoform 1 and isoform 2 are highly expressedin activated T-cells
Preparation and Storage
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(Western blot analysis of lysates from 293T, A431 cell line (from left to right), using IL4R Antibody (C-term). AAA284044 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)
WB (Western Blot)
(Western blot analysis of lysates from 293T, A431 cell line (from left to right), using IL4R Antibody (C-term). AAA284044 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of Ramos cells using IL4R Antibody (C-term)(green) compared to an isotype control of rabbit IgG(blue). AAA284044 was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.)
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of Ramos cells using IL4R Antibody (C-term)(green) compared to an isotype control of rabbit IgG(blue). AAA284044 was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.)
Related Product Information for anti-IL4R antibody
Receptor for both interleukin 4 and interleukin 13. Couples to the JAK1/2/3-STAT6 pathway. The IL4 response is involved in promoting Th2 differentiation. The IL4/IL13 responses are involved in regulating IgE production and, chemokine and mucus production at sites of allergic inflammation. In certain cell types, can signal through activation of insulin receptor substrates, IRS1/IRS2.
Product Categories/Family for anti-IL4R antibody
References
Idzerda R.L.,et al.J. Exp. Med. 171:861-873(1990).
Galizzi J.-P.,et al.Int. Immunol. 2:669-675(1990).
Kruse S.,et al.Int. Immunol. 11:1965-1970(1999).
Loftus B.J.,et al.Genomics 60:295-308(1999).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Galizzi J.-P.,et al.Int. Immunol. 2:669-675(1990).
Kruse S.,et al.Int. Immunol. 11:1965-1970(1999).
Loftus B.J.,et al.Genomics 60:295-308(1999).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Calculated MW: 89658
NCBI Official Full Name
interleukin-4 receptor subunit alpha isoform a
NCBI Official Synonym Full Names
interleukin 4 receptor
NCBI Official Symbol
IL4R
NCBI Official Synonym Symbols
CD124; IL4RA; IL-4RA
NCBI Protein Information
interleukin-4 receptor subunit alpha
UniProt Protein Name
Interleukin-4 receptor subunit alpha
UniProt Gene Name
IL4R
UniProt Synonym Gene Names
IL4RA; IL-4 receptor subunit alpha; IL-4R subunit alpha; IL-4R-alpha; IL-4RA; Soluble IL-4 receptor subunit alpha; Soluble IL-4R-alpha; sIL4Ralpha/prot; IL4-BP
UniProt Entry Name
IL4RA_HUMAN
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Product Notes
The IL4R il4r (Catalog #AAA284044) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The IL4R Antibody (C-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's IL4R can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the IL4R il4r for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IL4R, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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