WB (Western Blot)
(Detection of human and mouse ILK by western blot (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug), and mouse NIH 3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-ILK antibody AAA211385 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. ILK was also immunoprecipitated by rabbit anti-ILK antibody BL5116, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 10 seconds (A and B).)
Rabbit anti-Human, Mouse ILK Polyclonal Antibody | anti-ILK antibody
Rabbit anti-ILK Antibody, Affinity Purified
Gene Names
ILK; P59; ILK-1; ILK-2; p59ILK; HEL-S-28
Reactivity
Human, Mouse
Applications
Immunocytochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
ILK, Antibody; Rabbit anti-ILK Antibody, Affinity Purified; ILK-1; P59; integrin-linked protein kinase; p59ILK; HEL-S-28; epididymis secretory protein Li 28; 59 kDa serine/threonine-protein kinase; integrin-linked kinase-2; ILK-2; Integrin-linked protein kinase; integrin linked kinase; anti-ILK antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Concentration
200ug/ml (varies by lot)
Applicable Applications for anti-ILK antibody
ICC (Immunocytochemistry), IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Rat, Bovine, Orangutan
Immunogen
Between 402 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human and mouse ILK by western blot (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug), and mouse NIH 3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-ILK antibody AAA211385 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. ILK was also immunoprecipitated by rabbit anti-ILK antibody BL5116, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 10 seconds (A and B).)
WB (Western Blot)
(Detection of human and mouse ILK by western blot (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug), and mouse NIH 3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-ILK antibody AAA211385 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. ILK was also immunoprecipitated by rabbit anti-ILK antibody BL5116, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 10 seconds (A and B).)
ICC (Immunocytochemistry)
(Detection of human ILK by immunocytochemistry. Sample: Acetone-fixed asynchronous HeLa cells. Antibody: Affinity purified rabbit anti-ILK (Cat. No.AAA211385 Lot1) used at a dilution of 1:100 (2ug/ml). Detection: Red-fluorescent goat anti-rabbit IgG highly cross-adsorbed Antibody used at a dilution of 1:100.)
ICC (Immunocytochemistry)
(Detection of human ILK by immunocytochemistry. Sample: Acetone-fixed asynchronous HeLa cells. Antibody: Affinity purified rabbit anti-ILK (Cat. No.AAA211385 Lot1) used at a dilution of 1:100 (2ug/ml). Detection: Red-fluorescent goat anti-rabbit IgG highly cross-adsorbed Antibody used at a dilution of 1:100.)
Related Product Information for anti-ILK antibody
Integrin-linked kinase (ILK) is a serine/threonine kinase associated with the cytoplasmic domain of beta integrins and mediates integrin signaling from the extracellular matrix to the intracellular actin cytoskeleton. PKB/Akt and GSK-3beta are targets of ILK involved in integrin-mediated cell proliferation and cell survival. ILK has been shown to interact with Paxillin, PINCH-1, PINCH-2, and parvin to influence actin dynamics.
Product Categories/Family for anti-ILK antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
36,467 Da
NCBI Official Full Name
integrin-linked protein kinase isoform 1
NCBI Official Synonym Full Names
integrin linked kinase
NCBI Official Symbol
ILK
NCBI Official Synonym Symbols
P59; ILK-1; ILK-2; p59ILK; HEL-S-28
NCBI Protein Information
integrin-linked protein kinase
UniProt Protein Name
Integrin-linked protein kinase
UniProt Gene Name
ILK
UniProt Synonym Gene Names
ILK1; ILK2
UniProt Entry Name
ILK_HUMAN
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Product Notes
The ILK ilk (Catalog #AAA211385) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-ILK Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's ILK can be used in a range of immunoassay formats including, but not limited to, ICC (Immunocytochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the ILK ilk for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ILK, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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