Rabbit Insulin Receptor Polyclonal Antibody | anti-INSR antibody

Anti-Insulin Receptor Antibody, Rabbit Polyclonal

Cat. Num. AAA254673

• Reactivity: Human; Predicted to React with: Rat (Species predicted to react based on 100% sequence homology)
• Applications: Immunohistochemistry, Western Blot
• Purity: Protein A & Antigen Affinity
• Synonyms: Insulin Receptor, Antibody; Anti-Insulin Receptor Antibody, Rabbit Polyclonal; Insulin Receptor/INSR/CD220 Antibody, Rabbit PAb, Antigen Affinity Purified; insulin receptor; Anti-CD220 Antibody; Anti-HHF5 Antibody; Anti-Insulin Receptor Antibody; anti-INSR antibody

• Host: Rabbit
• Reactivity: Human; Predicted to React with: Rat (Species predicted to react based on 100% sequence homology)
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human Insulin Receptor
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-INSR antibody: IHC (Immunohistochemistry), WB (Western Blot)
• Immunogen: A synthetic peptide corresponding to the C-terminus of the Human Insulin Receptor/INSR/CD220
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human Insulin Receptor/INSR/CD220, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

IHC (Immunohiostchemistry)

(Immunochemical staining of mouse Insr in mouse liver with rabbit polyclonal antibody (1:300, formalin-fixed paraffin embedded sections).)

WB (Western Blot)

(Anti-Insr rabbit polyclonal antibody at 1:500 dilutionLane A: Rat brain tissue lysateLane B: Rat liver tissue lysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:156 kDaObserved band size:100 kDa)

Related Product Information for anti-INSR antibody

INSR (Insulin receptor), also known as CD22, is a transmembrane receptor that is activated by insulin. INSR belongs to the protein kinase superfamily and exists as a tetramer consisting of two alpha subunits and two beta subunits linked by disulfide bonds. The alpha and beta subunits are encoded by a single INSR gene, and the beta subunits pass through the cellular membrane. As the receptor for insulin with tyrosine-protein kinase activity, INSR associates with downstream mediators upon binding to insulin, including IRS1 (insulin receptor substrate 1) and phosphatidylinositol 3'-kinase (PI3K). IRS-1 binding and phosphorylation eventually lead to an increase in the high-affinity glucose transporter (Glut4) molecules on the outer membrane of insulin-responsive tissues. INSR isoform long and isoform short are expressed in the peripheral nerve, kidney, liver, striated muscle, fibroblasts and skin, and is found as a hybrid receptor with IGF1R which also binds IGF1 in muscle, heart, kidney, adipose tissue, skeletal muscle, hepatoma, fibroblasts, spleen, and placenta. Defects in Insulin Receptor/INSR are the cause of Rabson-Mendenhall syndrome (Mendenhall syndrome), insulin resistance (Ins resistance), leprechaunism (Donohue syndrome), and familial hyperinsulinemic hypoglycemia 5 (HHF5). It may also be associated with noninsulin-dependent diabetes mellitus (NIDDM).

Product Categories/Family for anti-INSR antibody

Receptor Tyrosine Kinases (RTKs); AMPK Signaling Pathway

References

Ebina Y., et al., (1985), The human insulin receptor cDNA: the structural basis for hormone-activated transmembrane signalling. Cell 40:747-758.Ullrich A., et al., (1985), Human insulin receptor and its relationship to the tyrosine kinase family of oncogenes.Nature 313:756-761.Grimwood J., et al., (2004), The DNA sequence and biology of human chromosome 19.Nature 428:529-535.

NCBI and Uniprot Product Information

• UniProt Accession #: P15127
• UniProt Protein Name: Insulin receptor
• UniProt Gene Name: Insr
• UniProt Synonym Gene Names: IR