DB (Dot Blot)
(Dot blot analysis of anti-INSR-pY1361 Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.)
Rabbit anti-Human Phospho-INSR Polyclonal Antibody | anti-INSR antibody
Phospho-INSR (Y1361) Antibody
Gene Names
INSR; HHF5; CD220
Reactivity
Human
Applications
Dot Blot, Immunohistochemistry
Synonyms
Phospho-INSR, Antibody; Phospho-INSR (Y1361) Antibody; Affinity Purified Rabbit Polyclonal Antibody (Pab); Insulin receptor; IR; CD220; Insulin receptor subunit alpha; Insulin receptor subunit beta; INSR; anti-INSR antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Clone Number
RB7155
Specificity
This INSR Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Y1361 of human INSR.
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Applicable Applications for anti-INSR antibody
DB (Dot Blot), IHC (Immunohistochemistry)
Antigen Source
Human
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
DB (Dot Blot)
(Dot blot analysis of anti-INSR-pY1361 Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.)
DB (Dot Blot)
(Dot blot analysis of anti-INSR-pY1361 Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.)
IHC (Immunohistochemistry)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
IHC (Immunohistochemistry)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
Related Product Information for anti-INSR antibody
After removal of the precursor signal peptide, the insulin receptor precursor is post-translationally cleaved into two chains (alpha and beta) that are covalently linked. Binding of insulin to the insulin receptor (INSR) stimulates glucose uptake, thereby mediating the metabolic functions of insulin. Binding to insulin stimulates association of the receptor with downstream mediators including IRS1 and phosphatidylinositol 3'-kinase (PI3K). This protein can activate PI3K either directly by binding to the p85 regulatory subunit, or indirectly via IRS1.
Product Categories/Family for anti-INSR antibody
References
Nakamaru, K., et al., Biochem. Biophys. Res. Commun. 328(2):449-454 (2005).Diaz, E., et al., Clin. Biochem. 38(3):243-247 (2005).McGettrick, A.J., et al., J. Biol. Chem. 280(8):6441-6446 (2005).Schmitt, T.L., et al., J. Biol. Chem. 280(5):3795-3801 (2005).Denley, A., et al., Mol. Endocrinol. 18(10):2502-2512 (2004).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
156,333 Da
NCBI Official Full Name
insulin receptor isoform Long preproprotein
NCBI Official Synonym Full Names
insulin receptor
NCBI Official Symbol
INSR
NCBI Official Synonym Symbols
HHF5; CD220
NCBI Protein Information
insulin receptor; IR
UniProt Protein Name
Insulin receptor
UniProt Gene Name
INSR
UniProt Synonym Gene Names
IR
UniProt Entry Name
INSR_HUMAN
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Product Notes
The INSR insr (Catalog #AAA290976) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-INSR (Y1361) Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Phospho-INSR can be used in a range of immunoassay formats including, but not limited to, DB (Dot Blot), IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the INSR insr for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Phospho-INSR, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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