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IHC (Immunohistochemistry)
(Anti- IRS1 antibody, AAA11632, IHC(P)IHC(P): Human Lung Cancer Tissue)
Rabbit Insulin receptor substrate 1 Polyclonal Antibody | anti-IRS1 antibody
Anti-IRS1 Antibody
Gene Names
IRS1; HIRS-1
Reactivity
Human, Mouse, Rat. No cross reactivity with other proteins.
Applications
Western Blot, Immunohistochemistry
Purity
Immunogen affinity purified.
Synonyms
Insulin receptor substrate 1; Polyclonal Antibody; Anti-IRS1 Antibody; HIRS 1 antibody; HIRS1 antibody; Insulin Receptor Substrate 1 antibody; IRS 1 antibody; IRS-1 antibody; IRS1 antibody; IRS1_HUMAN antibody; OTTHUMP00000164234 antibody; insulin receptor substrate 1; anti-IRS1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat. No cross reactivity with other proteins.
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Sequence Length
1242
Applicable Applications for anti-IRS1 antibody
Western Blot (WB), Immunohistochemistry (IHC-P)
Application Notes
Western Blot: Concentration: 0.1-0.5mug/ml; Tested Species: Human
Immunohistochemistry (Paraffin-embedded Section): Concentration: 0.5-1mug/ml; Tested Species: Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section): Concentration: 0.5-1mug/ml; Tested Species: Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Immunogen
E.coli-derived human IRS1 recombinant protein (Position: S1041-Q1242). Human IRS1 shares 78% and 80% amino acid (aa) sequences identity with mouse and rat IRS1, respectively.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
IHC (Immunohistochemistry)
(Anti- IRS1 antibody, AAA11632, IHC(P)IHC(P): Human Lung Cancer Tissue)
IHC (Immunohistochemistry)
(Anti- IRS1 antibody, AAA11632, IHC(P)IHC(P): Human Lung Cancer Tissue)
IHC (Immunohistchemistry)
(Anti- IRS1 antibody, AAA11632, IHC(P)IHC(P): Human Intestinal Cancer Tissue)
IHC (Immunohistchemistry)
(Anti- IRS1 antibody, AAA11632, IHC(P)IHC(P): Human Intestinal Cancer Tissue)
IHC (Immunohistochemistry)
(Anti- IRS1 antibody, AAA11632, IHC(P)IHC(P): Rat Skeletal Muscle Tissue)
IHC (Immunohistochemistry)
(Anti- IRS1 antibody, AAA11632, IHC(P)IHC(P): Rat Skeletal Muscle Tissue)
IHC (Immunohistochemistry)
(Anti- IRS1 antibody, AAA11632, IHC(P)IHC(P): Rat Intestine Tissue)
IHC (Immunohistochemistry)
(Anti- IRS1 antibody, AAA11632, IHC(P)IHC(P): Rat Intestine Tissue)
IHC (Immunohistochemistry)
(Anti- IRS1 antibody, AAA11632, IHC(P)IHC(P): Mouse Intestine Tissue)
IHC (Immunohistochemistry)
(Anti- IRS1 antibody, AAA11632, IHC(P)IHC(P): Mouse Intestine Tissue)
WB (Western Blot)
(Anti- IRS1 antibody, AAA11632, Western blottingAll lanes: Anti IRS1 (AAA11632) at 0.5ug/mlLane 1: A549 Whole Cell Lysate at 40ugLane 2: MM453 Whole Cell Lysate at 40ugLane 3: JURKAT Whole Cell Lysate at 40ugPredicted bind size: 132KDObserved bind size: 132KD)
WB (Western Blot)
(Anti- IRS1 antibody, AAA11632, Western blottingAll lanes: Anti IRS1 (AAA11632) at 0.5ug/mlLane 1: A549 Whole Cell Lysate at 40ugLane 2: MM453 Whole Cell Lysate at 40ugLane 3: JURKAT Whole Cell Lysate at 40ugPredicted bind size: 132KDObserved bind size: 132KD)
WB (Western Blot)
(Anti- IRS1 antibody, AAA11632, Western blottingAll lanes: Anti IRS1 (AAA11632) at 0.5ug/mlWB: Recombinant Human IRS1 Protein 0.5ngPredicted bind size: 39KDObserved bind size: 39KD)
WB (Western Blot)
(Anti- IRS1 antibody, AAA11632, Western blottingAll lanes: Anti IRS1 (AAA11632) at 0.5ug/mlWB: Recombinant Human IRS1 Protein 0.5ngPredicted bind size: 39KDObserved bind size: 39KD)
Related Product Information for anti-IRS1 antibody
Description: Rabbit IgG polyclonal antibody for Insulin receptor substrate 1(IRS1) detection. Tested with WB, IHC-P in Human;Mouse;Rat.
Background: Insulin receptor substrate 1(IRS-1) is a signalling adapter protein that in humans is encoded by the IRS-1 gene. It is mapped to 2q36.3. This gene exhibited no intrinsic enzyme activity, and it can serve as a docking protein involved in binding and activating other signal transduction molecules after being phosphorylated on tyrosine by insulin receptor kinase. IRS1 plays a key role in transmitting signals from the insulin and insulin-like growth factor-1(IGF-1) receptors to intracellular pathways PI3K/Akt and Erk MAP kinase pathways. IRS1 also has important biological function for both metabolic and mitogenic(growth promoting) pathways. In addition to those, IRS1 is a key regulator of PI3K within malignant cells.
Background: Insulin receptor substrate 1(IRS-1) is a signalling adapter protein that in humans is encoded by the IRS-1 gene. It is mapped to 2q36.3. This gene exhibited no intrinsic enzyme activity, and it can serve as a docking protein involved in binding and activating other signal transduction molecules after being phosphorylated on tyrosine by insulin receptor kinase. IRS1 plays a key role in transmitting signals from the insulin and insulin-like growth factor-1(IGF-1) receptors to intracellular pathways PI3K/Akt and Erk MAP kinase pathways. IRS1 also has important biological function for both metabolic and mitogenic(growth promoting) pathways. In addition to those, IRS1 is a key regulator of PI3K within malignant cells.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
131,591 Da
NCBI Official Full Name
insulin receptor substrate 1
NCBI Official Synonym Full Names
insulin receptor substrate 1
NCBI Official Symbol
IRS1
NCBI Official Synonym Symbols
HIRS-1
NCBI Protein Information
insulin receptor substrate 1; IRS-1
UniProt Protein Name
Insulin receptor substrate 1
UniProt Gene Name
IRS1
UniProt Synonym Gene Names
IRS-1
UniProt Entry Name
IRS1_HUMAN
Similar Products
Product Notes
The IRS1 irs1 (Catalog #AAA11632) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-IRS1 Antibody reacts with Human, Mouse, Rat. No cross reactivity with other proteins. and may cross-react with other species as described in the data sheet. AAA Biotech's Insulin receptor substrate 1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC-P). Western Blot: Concentration: 0.1-0.5mug/ml; Tested Species: Human Immunohistochemistry (Paraffin-embedded Section): Concentration: 0.5-1mug/ml; Tested Species: Human, Mouse, Rat. Researchers should empirically determine the suitability of the IRS1 irs1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Insulin receptor substrate 1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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