FCM/FACS (Flow Cytometry)
(Mouse Irak1 Antibody (Center) flow cytometric analysis of Neuro-2a cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit anti-Mouse Irak1 Polyclonal Antibody | anti-Irak1 antibody
Mouse Irak1 Antibody (Center)
Gene Names
Irak1; IRAK; Plpk; mPLK; IRAK-1; Il1rak; IRAK1-S; AA408924
Reactivity
Mouse
Applications
Flow Cytometry, Functional Assay, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
Irak1, Antibody; Mouse Irak1 Antibody (Center); Interleukin-1 receptor-associated kinase 1; IRAK; IRAK-1; Pelle-like protein kinase; mPLK; Irak1; Il1rak; anti-Irak1 antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This Mouse Irak1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 405-433 amino acids from the Central region of mouse Irak1.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
405-433
Sequence Length
712
Applicable Applications for anti-Irak1 antibody
FCM/FACS (Flow Cytometry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
MOUSE
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(Mouse Irak1 Antibody (Center) flow cytometric analysis of Neuro-2a cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(Mouse Irak1 Antibody (Center) flow cytometric analysis of Neuro-2a cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)
WB (Western Blot)
(Mouse Irak1 Antibody (Center) western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).This demonstrates the Irak1 antibody detected the Irak1 protein (arrow).)
WB (Western Blot)
(Mouse Irak1 Antibody (Center) western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).This demonstrates the Irak1 antibody detected the Irak1 protein (arrow).)
Related Product Information for anti-Irak1 antibody
Irak1 binds to the IL-1 type I receptor following IL-1 engagement, triggering intracellular signaling cascades leading to transcriptional up-regulation and mRNA stabilization. The kinase activity of this enzyme may not be required for IL-1 signaling. Once phosphorylated, IRAK1 recruits the adapter protein PELI1.
Product Categories/Family for anti-Irak1 antibody
References
Vaughan, T., et al. Mol. Immunol. 47(15):2515-2518(2010)
Hoshino, K., et al. J. Immunol. 184(7):3341-3345(2010)
Dong, J.W., et al. Am. J. Physiol. Heart Circ. Physiol. 298 (3), H1079-H1087 (2010) :
Gan, L., et al. Mol. Immunol. 47(6):1278-1282(2010)
Maitra, U., et al. J. Biol. Chem. 284(51):35403-35411(2009)
Hoshino, K., et al. J. Immunol. 184(7):3341-3345(2010)
Dong, J.W., et al. Am. J. Physiol. Heart Circ. Physiol. 298 (3), H1079-H1087 (2010) :
Gan, L., et al. Mol. Immunol. 47(6):1278-1282(2010)
Maitra, U., et al. J. Biol. Chem. 284(51):35403-35411(2009)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
77269
NCBI Official Full Name
interleukin-1 receptor-associated kinase 1 isoform 4
NCBI Official Synonym Full Names
interleukin-1 receptor-associated kinase 1
NCBI Official Symbol
Irak1
NCBI Official Synonym Symbols
IRAK; Plpk; mPLK; IRAK-1; Il1rak; IRAK1-S; AA408924
NCBI Protein Information
interleukin-1 receptor-associated kinase 1
UniProt Protein Name
Interleukin-1 receptor-associated kinase 1
UniProt Gene Name
Irak1
UniProt Synonym Gene Names
Il1rak; IRAK; IRAK-1; mPLK
UniProt Entry Name
IRAK1_MOUSE
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Product Notes
The Irak1 irak1 (Catalog #AAA287150) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 405-433. The Mouse Irak1 Antibody (Center) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Irak1 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the Irak1 irak1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Irak1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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