IP (Immunoprecipitation)
(Detection of mouse IRF8 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 mg for IP, 20% of IP loaded) from RAW 264.7 cells. Antibodies: Affinity purified rabbit anti-IRF8 antibody AAA212457 (lot AAA212457-1) used for IP at 6 ug/mg lysate. IRF8 was also immunoprecipitated by rabbit anti-IRF8 antibodies BL12057, and For blotting immunoprecipitated IRF8, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Rabbit anti-Mouse IRF8 Polyclonal Antibody | anti-IRF8 antibody
Rabbit anti-IRF8 Antibody, Affinity Purified
Gene Names
Irf8; Myls; ICSBP; IRF-8; Icsbp1; AI893568
Reactivity
Mouse
Applications
Chromatin Immunoprecipitation, Immunoprecipitation, Immunoprecipitation
Purity
Antigen Affinity Purified
Synonyms
IRF8, Antibody; Rabbit anti-IRF8 Antibody, Affinity Purified; interferon regulatory factor 8; IRF-8; interferon consensus sequence-binding protein; interferon consensus sequence binding protein 1; Icsbp1; ICSBP; AI893568; Myls; Interferon regulatory factor 8; anti-IRF8 antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-IRF8 antibody
ChIP (Chromatin immunoprecipitation), IP (Immunoprecipitation)
Immunogen
Between 225 and 275
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
IP (Immunoprecipitation)
(Detection of mouse IRF8 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 mg for IP, 20% of IP loaded) from RAW 264.7 cells. Antibodies: Affinity purified rabbit anti-IRF8 antibody AAA212457 (lot AAA212457-1) used for IP at 6 ug/mg lysate. IRF8 was also immunoprecipitated by rabbit anti-IRF8 antibodies BL12057, and For blotting immunoprecipitated IRF8, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of mouse IRF8 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 mg for IP, 20% of IP loaded) from RAW 264.7 cells. Antibodies: Affinity purified rabbit anti-IRF8 antibody AAA212457 (lot AAA212457-1) used for IP at 6 ug/mg lysate. IRF8 was also immunoprecipitated by rabbit anti-IRF8 antibodies BL12057, and For blotting immunoprecipitated IRF8, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
ChIP (Chromatin Immunoprecipitation)
(Localization of IRF8 binding sites by ChIP-Sequencing. Chromatin from mouse bone marrow dendritic cells (BMDCs) was immunoprecipitated with anti-IRF8 AAA212457 (lot AAA212457-1) and analyzed by DNA sequencing according to methods available at http://www.weizmann.ac.il/immunology/AmitLab/data-and-method/iChIP/method. The figure illustrates the peak distribution of IRF8 binding within a 156 kb region of chromosome 19 (blue) as detected by anti-IRF8 AAA212457.)
ChIP (Chromatin Immunoprecipitation)
(Localization of IRF8 binding sites by ChIP-Sequencing. Chromatin from mouse bone marrow dendritic cells (BMDCs) was immunoprecipitated with anti-IRF8 AAA212457 (lot AAA212457-1) and analyzed by DNA sequencing according to methods available at http://www.weizmann.ac.il/immunology/AmitLab/data-and-method/iChIP/method. The figure illustrates the peak distribution of IRF8 binding within a 156 kb region of chromosome 19 (blue) as detected by anti-IRF8 AAA212457.)
Related Product Information for anti-IRF8 antibody
Interferon regulatory factor (IRF8) is a member of the IRF family of transcription factors that plays a critical role in immune cell function and development. IRF8 has been shown to be an important regulator of CD8 T-cell differentiation.
Product Categories/Family for anti-IRF8 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
48,237 Da
NCBI Official Full Name
interferon regulatory factor 8
NCBI Official Synonym Full Names
interferon regulatory factor 8
NCBI Official Symbol
Irf8
NCBI Official Synonym Symbols
Myls; ICSBP; IRF-8; Icsbp1; AI893568
NCBI Protein Information
interferon regulatory factor 8; interferon consensus sequence binding protein 1; interferon consensus sequence-binding protein
UniProt Protein Name
Interferon regulatory factor 8
UniProt Gene Name
Irf8
UniProt Synonym Gene Names
Icsbp; Icsbp1; IRF-8; ICSBP
UniProt Entry Name
IRF8_MOUSE
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Product Notes
The IRF8 irf8 (Catalog #AAA212457) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-IRF8 Antibody, Affinity Purified reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's IRF8 can be used in a range of immunoassay formats including, but not limited to, ChIP (Chromatin immunoprecipitation), IP (Immunoprecipitation). Researchers should empirically determine the suitability of the IRF8 irf8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IRF8, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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