WB (Western Blot)
(Western blot analysis of lysates from PC12 cells, primary antibody was diluted at 1:1000, 4 degree over night)
Rabbit anti-Human, Mouse ISG15 Polyclonal Antibody | anti-ISG15 antibody
ISG15 Rabbit Polyclonal Antibody
Gene Names
ISG15; G1P2; IP17; UCRP; IFI15; hUCRP
Reactivity
Human, Mouse
Applications
ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Synonyms
ISG15, Antibody; ISG15 Rabbit Polyclonal Antibody; BH6842; Ubiquitin-like protein ISG15 (Interferon-induced 15 kDa protein)(Interferon-induced 17 kDa protein)(IP17)(Ubiquitin cross-reactive protein)(hUCRP); anti-ISG15 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Specificity
ISG15 Polyclonal Antibody detects endogenous levels of protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form/Format
Liquid in PBS containing 50% glycerol, and 0.02% sodium azide.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-ISG15 antibody
ELISA, IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
Synthesized peptide derived from part region of human protein
Modification
Unmodified/Total
Preparation and Storage
Store at -20 degree C/1 year
WB (Western Blot)
(Western blot analysis of lysates from PC12 cells, primary antibody was diluted at 1:1000, 4 degree over night)
WB (Western Blot)
(Western blot analysis of lysates from PC12 cells, primary antibody was diluted at 1:1000, 4 degree over night)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded mouse-liver tissue. 1,ISG15 Polyclonal Antibody was diluted at 1:200(4 degree overnight). 2, EDTA pH 9.0 was used for antigen retrieval(>98 degree C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min) Negtive control was used by secondary antibody only.))
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded mouse-liver tissue. 1,ISG15 Polyclonal Antibody was diluted at 1:200(4 degree overnight). 2, EDTA pH 9.0 was used for antigen retrieval(>98 degree C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min) Negtive control was used by secondary antibody only.))
Related Product Information for anti-ISG15 antibody
The protein encoded by this gene is a ubiquitin-like protein that is conjugated to intracellular target proteins upon activation by interferon-alpha and interferon-beta. Several functions have been ascribed to the encoded protein, including chemotactic activity towards neutrophils, direction of ligated target proteins to intermediate filaments, cell-to-cell signaling, and antiviral activity during viral infections. While conjugates of this protein have been found to be noncovalently attached to intermediate filaments, this protein is sometimes secreted. [provided by RefSeq, Dec 2012]
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
17,888 Da
NCBI Official Full Name
ubiquitin-like protein ISG15
NCBI Official Synonym Full Names
ISG15 ubiquitin-like modifier
NCBI Official Symbol
ISG15
NCBI Official Synonym Symbols
G1P2; IP17; UCRP; IFI15; hUCRP
NCBI Protein Information
ubiquitin-like protein ISG15; ubiquitin cross-reactive protein; interferon-stimulated protein, 15 kDa; interferon-induced 17-kDa/15-kDa protein; interferon, alpha-inducible protein (clone IFI-15K)
UniProt Protein Name
Ubiquitin-like protein ISG15
UniProt Gene Name
ISG15
UniProt Synonym Gene Names
G1P2; UCRP; IP17; hUCRP
UniProt Entry Name
ISG15_HUMAN
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Product Notes
The ISG15 isg15 (Catalog #AAA318968) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ISG15 Rabbit Polyclonal Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's ISG15 can be used in a range of immunoassay formats including, but not limited to, ELISA, IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the ISG15 isg15 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ISG15, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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