WB (Western Blot)
(Western blot analysis of ISG15 (arrow) using rabbit polyclonal ISG15 Antibody (C-term N151). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ISG15 gene.)
Rabbit anti-Human ISG15 Polyclonal Antibody | anti-ISG15 antibody
ISG15 Antibody (C-term)
Gene Names
ISG15; G1P2; IP17; UCRP; IFI15; IMD38; hUCRP
Reactivity
Human
Applications
ELISA, Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
ISG15, Antibody; ISG15 Antibody (C-term); Ubiquitin-like protein ISG15; Interferon-induced 15 kDa protein; Interferon-induced 17 kDa protein; IP17; Ubiquitin cross-reactive protein; hUCRP; ISG15; G1P2; UCRP; anti-ISG15 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This ISG15 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 136-165 amino acids from the C-terminal region of human ISG15.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Concentration
Vial Concentration: 2 (varies by lot)
Sequence Positions
136-165
Sequence Length
165
Applicable Applications for anti-ISG15 antibody
ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(Western blot analysis of ISG15 (arrow) using rabbit polyclonal ISG15 Antibody (C-term N151). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ISG15 gene.)
WB (Western Blot)
(Western blot analysis of ISG15 (arrow) using rabbit polyclonal ISG15 Antibody (C-term N151). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ISG15 gene.)
WB (Western Blot)
(Western blot analysis of ISG15 Antibody (C-term N151) in HL-60 cell line lysates (35ug/lane). ISG15 (arrow) was detected using the purified Pab.)
WB (Western Blot)
(Western blot analysis of ISG15 Antibody (C-term N151) in HL-60 cell line lysates (35ug/lane). ISG15 (arrow) was detected using the purified Pab.)
Related Product Information for anti-ISG15 antibody
ISG15 is secreted from monocytes in response to type I interferons and causes natural killer (NK)-cell proliferation and an augmentation of non-MCH (major histocompatibility complex)-restricted cytotoxicity. Synthesis is stimulated by IFN-alpha or IFN-beta or IFN-omega, but not IFN-gamma. ISG15 expression is also induced by overexpression of interferon regulatory factors that participate in transcriptional regulation of IFN genes, and by influenza B virus. ISG15 is secreted also by cell lines of monocyte, T-lymphocyte, B-lymphocyte, human fibroblasts, and epithelial origins. The induction of terminal differentiation in human melanoma cells is associated with alterations in ISG15 expression. Enhancement of NK cell proliferation, augmentation of non-major histocompatibility complex-restricted cytotoxicity, and induction of IFN-gamma from T cells identify ISG15 as a member of the cytokine cascade and suggest that it may be responsible for amplifying and directing some of the immunomodulatory effects of IFN-alpha or IFN-beta. ISG15 has has also been shown to function intracellularly as a ubiquitin homolog.
Product Categories/Family for anti-ISG15 antibody
References
Padovan, E., et al., Cancer Res. 62(12):3453-3458 (2002).
Meraro, D., et al., J. Immunol. 168(12):6224-6231 (2002).
Reich, N., et al., Proc. Natl. Acad. Sci. U.S.A. 84(18):6394-6398 (1987).
Blomstrom, D.C., et al., J. Biol. Chem. 261(19):8811-8816 (1986).
Clauss, I.M., et al., Cytogenet. Cell Genet. 53 (2-3), 166-168 (1990).
Meraro, D., et al., J. Immunol. 168(12):6224-6231 (2002).
Reich, N., et al., Proc. Natl. Acad. Sci. U.S.A. 84(18):6394-6398 (1987).
Blomstrom, D.C., et al., J. Biol. Chem. 261(19):8811-8816 (1986).
Clauss, I.M., et al., Cytogenet. Cell Genet. 53 (2-3), 166-168 (1990).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
17888
NCBI Official Full Name
ubiquitin-like protein ISG15
NCBI Official Synonym Full Names
ISG15 ubiquitin-like modifier
NCBI Official Symbol
ISG15
NCBI Official Synonym Symbols
G1P2; IP17; UCRP; IFI15; IMD38; hUCRP
NCBI Protein Information
ubiquitin-like protein ISG15
UniProt Protein Name
Ubiquitin-like protein ISG15
UniProt Gene Name
ISG15
UniProt Synonym Gene Names
G1P2; UCRP; IP17; hUCRP
UniProt Entry Name
ISG15_HUMAN
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Product Notes
The ISG15 isg15 (Catalog #AAA289156) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 136-165. The ISG15 Antibody (C-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ISG15 can be used in a range of immunoassay formats including, but not limited to, ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the ISG15 isg15 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ISG15, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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