Rabbit ITGA4 Polyclonal Antibody | anti-ITGA4 antibody

ITGA4 Antibody

Cat. Num. AAA325918

• Gene Names: ITGA4; IA4; CD49D
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Immunohistochemistry, Western Blot
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Synonyms: ITGA4, Antibody; ITGA4 Antibody; 269C wild type; Antigen CD49D, alpha 4 subunit of VLA 4 receptor; CD49 antigen like family member D; CD49 antigen-like family member D; CD49d; IA4; Integrin alpha 4; Integrin alpha 4 subunit; Integrin alpha IV; Integrin alpha-4; Integrin alpha-IV; Integrin, alpha 4 (antigen CD49D, alpha 4 subunit of VLA 4 receptor); ITA4_HUMAN; ITGA4; MGC90518; OTTHUMP00000205320; Very late activation protein 4 receptor, alpha 4 subunit; VLA 4 subunit alpha; VLA-4 subunit alpha; VLA4; anti-ITGA4 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: ITGA4 antibody detects endogenous levels of total ITGA4
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 1032

• Applicable Applications for anti-ITGA4 antibody: ELISA, IHC (Immunohistochemistry), WB (Western Blot)
• Immunogen: A synthesized peptide derived from human ITGA4
• Subcellular Location: Membrane.
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

IHC (Immunohiostchemistry)

(AAA325918 at 1/100 staining Human pancreas tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

WB (Western Blot)

(Western blot analysis of Jurkat lysate using ITGA4 antibody. The lane on the left is treated with the antigen-specific peptide.)

Related Product Information for anti-ITGA4 antibody

Description: Integrins are alpha/beta heterodimeric cell surface receptors that play a pivotal role in cell adhesion and migration, as well as in growth and survival (1, 2). The integrin family contains at least 18 alpha and 8 beta subunits that form 24 known integrins with distinct tissue distribution and overlapping ligand specificities (3). Integrins not only transmit signals to cells in response to the extracellular environment (outside-in signaling), but also sense intracellular cues to alter their interaction with the extracellular environment (inside-out signaling) (1, 2).A pair of important alpha4 integrins, alpha4beta1 and alpha4beta7, interact with VCAM-1, fibronectin, and MAdCAM-1 at cell adhesions (3). Gene knockout and antibody blocking research reveal that alpha4 integrins play important roles in embryonic liver and heart development and in fetal lymphocyte homing (4-6). Phosphorylation at Ser988 within the cytoplasmic tail of integrin alpha4 blocks binding to paxillin and promotes leading edge migration (7, 8).On SDS-PAGE, integrin alpha4 can migrate at several different apparent molecular sizes, a 150 kDa mature protein and a 140 kDa precursor protein (a 180 kDa protein also exists under mild non-reducing conditions) (9). Integrin alpha4 has a cleavage site at Arg558, which results in a small portion of the protein as either an 80 kDa N-terminal or 70 kDa C-terminal fragment (10).
Function: Integrins alpha-4/beta-1 (VLA-4) and alpha-4/beta-7 are receptors for fibronectin. They recognize one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. They are also receptors for VCAM1. Integrin alpha-4/beta-1 recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-4/beta-7 is also a receptor for MADCAM1. It recognizes the sequence L-D-T in MADCAM1. On activated endothelial cells integrin VLA-4 triggers homotypic aggregation for most VLA-4-positive leukocyte cell lines. It may also participate in cytolytic T-cell interactions with target cells. ITGA4:ITGB1 binds to fractalkine (CX3CL1) and may act as its coreceptor in CX3CR1-dependent fractalkine signaling (PubMed:23125415). ITGA4:ITGB1 binds to PLA2G2A via a site (site 2) which is distinct from the classical ligand-binding site (site 1) and this induces integrin conformational changes and enhanced ligand binding to site 1 (PubMed:18635536, PubMed:25398877).
Subunit Structure: Heterodimer of an alpha and a beta subunit. The alpha subunit can sometimes be cleaved into two non-covalently associated fragments. Alpha-4 associates with either beta-1 or beta-7. Alpha-4 interacts with PXN, LPXN, and TGFB1I1/HIC5. Interacts with CSPG4 through CSPG4 chondroitin sulfate glycosaminoglycan. Interacts with JAML; integrin alpha-4/beta-1 may regulate leukocyte to endothelial cells adhesion by controlling JAML homodimerization. ITGA4:ITGB1 is found in a ternary complex with CX3CR1 and CX3CL1 (PubMed:23125415).
Post-translational Modifications: Phosphorylation on Ser-1027 inhibits PXN binding.
Similarity: The SG1 motif is involved in binding to chondroitin sulfate glycosaminoglycan and cell adhesion. Belongs to the integrin alpha chain family.

NCBI and Uniprot Product Information

• NCBI GI #: 67191027
• NCBI GeneID: 3676
• NCBI Accession #: NP_000876.3
• NCBI GenBank Nucleotide #: NM_000885.5
• UniProt Accession #: P13612; Q7Z4L6; D3DPG4
• Molecular Weight: Observed: 115 kDa; Predicted: 115 kDa
• NCBI Official Full Name: integrin alpha-4 isoform 1 preproprotein
• NCBI Official Synonym Full Names: integrin subunit alpha 4
• NCBI Official Symbol: ITGA4
• NCBI Official Synonym Symbols: IA4; CD49D
• NCBI Protein Information: integrin alpha-4
• UniProt Protein Name: Integrin alpha-4
• UniProt Gene Name: ITGA4
• UniProt Synonym Gene Names: CD49D