FCM/FACS (Flow Cytometry)
(ITIH2 Antibody (C-term) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit ITIH2 Polyclonal Antibody | anti-ITIH2 antibody
ITIH2 Antibody (C-term)
Gene Names
ITIH2; H2P; SHAP
Reactivity
Human (Predicted Reactivity: Pig)
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, ELISA, Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
ITIH2, Antibody; ITIH2 Antibody (C-term); Inter-alpha-trypsin inhibitor heavy chain H2; ITI heavy chain H2; ITI-HC2; Inter-alpha-inhibitor heavy chain 2; Inter-alpha-trypsin inhibitor complex component II; Serum-derived hyaluronan-associated protein; SHAP; ITIH2; IGHEP2; anti-ITIH2 antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Pig)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This ITIH2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 858-886 amino acids from the C-terminal region of human ITIH2.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Concentration
Vial Concentration: 2 (varies by lot)
Sequence Positions
858-886
Sequence Length
946
Applicable Applications for anti-ITIH2 antibody
FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(ITIH2 Antibody (C-term) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(ITIH2 Antibody (C-term) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with ITIH2 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with ITIH2 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
WB (Western Blot)
(Western blot analysis of ITIH2 antibody (C-term) in HepG2 cell line lysates (35ug/lane). ITIH2 (arrow) was detected using the purified Pab.)
WB (Western Blot)
(Western blot analysis of ITIH2 antibody (C-term) in HepG2 cell line lysates (35ug/lane). ITIH2 (arrow) was detected using the purified Pab.)
Related Product Information for anti-ITIH2 antibody
ITIH2 may act as a carrier of hyaluronan in serum or as a binding protein between hyaluronan and other matrix protein, including those on cell surfaces in tissues to regulate the localization, synthesis and degradation of hyaluronan which are essential to cells undergoing biological processes.
Product Categories/Family for anti-ITIH2 antibody
References
Obayashi,Y., Oncol. Rep. 19 (5), 1245-1251 (2008)
Kishida,T., Connect. Tissue Res. 49 (2), 105-108 (2008)
Kishida,T., Connect. Tissue Res. 49 (2), 105-108 (2008)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
106463
NCBI Official Full Name
inter-alpha-trypsin inhibitor heavy chain H2
NCBI Official Synonym Full Names
inter-alpha-trypsin inhibitor heavy chain 2
NCBI Official Symbol
ITIH2
NCBI Official Synonym Symbols
H2P; SHAP
NCBI Protein Information
inter-alpha-trypsin inhibitor heavy chain H2
UniProt Protein Name
Inter-alpha-trypsin inhibitor heavy chain H2
UniProt Gene Name
ITIH2
UniProt Synonym Gene Names
IGHEP2; ITI heavy chain H2; ITI-HC2; Inter-alpha-inhibitor heavy chain 2; SHAP
UniProt Entry Name
ITIH2_HUMAN
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Product Notes
The ITIH2 itih2 (Catalog #AAA288212) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 858-886. The ITIH2 Antibody (C-term) reacts with Human (Predicted Reactivity: Pig) and may cross-react with other species as described in the data sheet. AAA Biotech's ITIH2 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the ITIH2 itih2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ITIH2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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