WB (Western Blot)
(Detection of human JMJD1B by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-JMJD1B antibody AAA211198 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate (B). JMJD1B was also immunoprecipitated by rabbit anti-JMJD1B antibody BL3820, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).)
Rabbit anti-Human JMJD1B Polyclonal Antibody | anti-JMJD1B antibody
Rabbit anti-JMJD1B Antibody, Affinity Purified
Gene Names
KDM3B; 5qNCA; NET22; C5orf7; JMJD1B
Reactivity
Human
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
JMJD1B, Antibody; Rabbit anti-JMJD1B Antibody, Affinity Purified; jumonji domain-containing protein 1B; NET22; nuclear protein 5qNCA; lysine-specific demethylase 3B; lysine (K)-specific demethylase 3B; JMJD1B; jmjC domain-containing histone demethylation protein 2B; 5qNCA; jumonji domain containing 1B; C5orf7; Lysine-specific demethylase 3B; lysine demethylase 3B; anti-JMJD1B antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Concentration
200ug/ml (varies by lot)
Applicable Applications for anti-JMJD1B antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Mouse
Immunogen
Between 1711 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human JMJD1B by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-JMJD1B antibody AAA211198 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate (B). JMJD1B was also immunoprecipitated by rabbit anti-JMJD1B antibody BL3820, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).)
WB (Western Blot)
(Detection of human JMJD1B by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-JMJD1B antibody AAA211198 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate (B). JMJD1B was also immunoprecipitated by rabbit anti-JMJD1B antibody BL3820, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).)
IHC (Immunohistochemistry)
(Detection of human JMJD1B by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-JMJD1B (Cat. No. AAA211198 Lot1) used at a dilution of 1:200 (1ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human JMJD1B by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-JMJD1B (Cat. No. AAA211198 Lot1) used at a dilution of 1:200 (1ug/ml). Detection: DAB)
Related Product Information for anti-JMJD1B antibody
JMJD1B is a member of the jumonji (jmj) domain containing gene family of histone demethylases that plays a role in chromatin regulation and influences transcriptional activation and suppression. Some recently characterized members of the jmj family include JARID1A/RBP2, JARID1C, JMJD1A, JMJD1B, JMJD1C, JMJD2A, JMJD2C and JMJD2D. JMJD1B was identified as the 5qNCA gene that lies within a locus on chromosome 5 that is frequently deleted in myeloid leukemias and myelodysplasias. JMJD1B has been demonstrated to have growth suppressive activity and is implicated as a tumor suppressor gene.
Product Categories/Family for anti-JMJD1B antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
84,712 Da
NCBI Official Full Name
lysine-specific demethylase 3B
NCBI Official Synonym Full Names
lysine (K)-specific demethylase 3B
NCBI Official Symbol
KDM3B
NCBI Official Synonym Symbols
5qNCA; NET22; C5orf7; JMJD1B
NCBI Protein Information
lysine-specific demethylase 3B; nuclear protein 5qNCA; jumonji domain containing 1B; jumonji domain-containing protein 1B; jmjC domain-containing histone demethylation protein 2B
UniProt Protein Name
Lysine-specific demethylase 3B
UniProt Gene Name
KDM3B
UniProt Synonym Gene Names
C5orf7; JHDM2B; JMJD1B; KIAA1082
UniProt Entry Name
KDM3B_HUMAN
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Product Notes
The JMJD1B kdm3b (Catalog #AAA211198) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-JMJD1B Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's JMJD1B can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the JMJD1B kdm3b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "JMJD1B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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