Application Data
(HTRF assay for JMJD1B / KDM3B activity 3.3 uM H3K9me2 peptide was incubated with different concentrations of JMJD1B / KDM3B protein in a 10 ul reaction system containing 50 mM HEPES pH 7.5, 1 mM TCEP, 0.02% Triton X100, 100 uM 2-oxoglutarate, 100 uM ascorbate, 50 ?M (NH4)2Fe(SO4)2·6H2O and for 1 hours at room temperature, then 10 ?l of H3K9me0 antibody and SA-XL665 mixture (1:100 dilution in the same buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
JMJD1B/KDM3B recombinant protein
Recombinant JMJD1B/KDM3B protein
Gene Names
KDM3B; 5qNCA; NET22; C5orf7; JMJD1B
Purity
The recombinant protein is >85% pure by SDS-PAGE.
Synonyms
JMJD1B/KDM3B; N/A; Recombinant JMJD1B/KDM3B protein; Lysine Demethylase 3B; Jumonji Domain-Containing Protein 1B; Nuclear Protein 5qNCA; JMJD1B/KDM3B recombinant protein
Host
Baculovirus
Purity/Purification
The recombinant protein is >85% pure by SDS-PAGE.
Form/Format
Full length recombinant JMJD1B/KDM3B protein expressed in Sf9 cells and supplied in 25mM HEPES-NaOH pH7.5, 300mM NaCl, 0.04% Triton X100, 10% glycerol, and 0.5mM TCEP.
Protein Species
Human
Tag
DYKDDDDK-Tag, His-Tag
Notes
Recombinant JMJD1B/KDM3B is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling.
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Shipping Temp: Dry Ice
Shipping Temp: Dry Ice
Application Data
(HTRF assay for JMJD1B / KDM3B activity 3.3 uM H3K9me2 peptide was incubated with different concentrations of JMJD1B / KDM3B protein in a 10 ul reaction system containing 50 mM HEPES pH 7.5, 1 mM TCEP, 0.02% Triton X100, 100 uM 2-oxoglutarate, 100 uM ascorbate, 50 ?M (NH4)2Fe(SO4)2·6H2O and for 1 hours at room temperature, then 10 ?l of H3K9me0 antibody and SA-XL665 mixture (1:100 dilution in the same buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
Application Data
(HTRF assay for JMJD1B / KDM3B activity 3.3 uM H3K9me2 peptide was incubated with different concentrations of JMJD1B / KDM3B protein in a 10 ul reaction system containing 50 mM HEPES pH 7.5, 1 mM TCEP, 0.02% Triton X100, 100 uM 2-oxoglutarate, 100 uM ascorbate, 50 ?M (NH4)2Fe(SO4)2·6H2O and for 1 hours at room temperature, then 10 ?l of H3K9me0 antibody and SA-XL665 mixture (1:100 dilution in the same buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
Application Data
(HTRF assay for JMJD1B / KDM3B activity 3.3 uM H3K9me2 peptide was incubated with different concentrations of JMJD1B / KDM3B protein in a 10 ul reaction system containing 50 mM HEPES pH 7.5, 1 mM TCEP, 0.02% Triton X100, 100 uM 2-oxoglutarate, 100 uM ascorbate, 50 ?M (NH4)2Fe(SO4)2·6H2O and for 1 hours at room temperature, then 10 ?l of H3K9me0 antibody and SA-XL665 mixture (1:100 dilution in the same buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
Application Data
(HTRF assay for JMJD1B / KDM3B activity 3.3 uM H3K9me2 peptide was incubated with different concentrations of JMJD1B / KDM3B protein in a 10 ul reaction system containing 50 mM HEPES pH 7.5, 1 mM TCEP, 0.02% Triton X100, 100 uM 2-oxoglutarate, 100 uM ascorbate, 50 ?M (NH4)2Fe(SO4)2·6H2O and for 1 hours at room temperature, then 10 ?l of H3K9me0 antibody and SA-XL665 mixture (1:100 dilution in the same buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
SDS-PAGE
(Recombinant JMJD1B / KDM3B protein gel. JMJD1B / KDM3B protein was run on an 10% SDS-PAGE gel and stained with Coomassie blue. MW: 195 kDa Purity: >85%)
SDS-PAGE
(Recombinant JMJD1B / KDM3B protein gel. JMJD1B / KDM3B protein was run on an 10% SDS-PAGE gel and stained with Coomassie blue. MW: 195 kDa Purity: >85%)
Related Product Information for JMJD1B/KDM3B recombinant protein
Short Description: Recombinant JMJD1B/KDM3B (accession number NP_057688.2) was expressed in Sf9 cells and contains an N-terminal DYKDDDDK tag with a molecular weight of 193.4 kDa. Lysine (K)-specific demethylase 3B (KDM3B), also known as Jumonji domain-containing protein 1B (JMJD1B), is a histone demethylase that specifically demethylates lysine 9 of histone H3 (H3K9), thereby playing a central role in defining the histone code. Demethylation of lysine residues generates formaldehyde and succinate. KDM3B may have tumor suppressor activity. Recombinant JMJD1B/KDM3B can be used for enzyme kinetics and inhibitor screening studies.
Background: Lysine (K)-specific demethylase 3B (KDM3B), also known as Jumonji domain-containing protein 1B (JMJD1B), is a histone demethylase that specifically demethylates lysine 9 of histone H3 (H3K9), thereby playing a central role in defining the histone code. Demethylation of lysine residues generates formaldehyde and succinate. KDM3B may have tumor suppressor activity.
Background: Lysine (K)-specific demethylase 3B (KDM3B), also known as Jumonji domain-containing protein 1B (JMJD1B), is a histone demethylase that specifically demethylates lysine 9 of histone H3 (H3K9), thereby playing a central role in defining the histone code. Demethylation of lysine residues generates formaldehyde and succinate. KDM3B may have tumor suppressor activity.
Product Categories/Family for JMJD1B/KDM3B recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
84,712 Da
NCBI Official Full Name
lysine-specific demethylase 3B
NCBI Official Synonym Full Names
lysine (K)-specific demethylase 3B
NCBI Official Symbol
KDM3B
NCBI Official Synonym Symbols
5qNCA; NET22; C5orf7; JMJD1B
NCBI Protein Information
lysine-specific demethylase 3B; nuclear protein 5qNCA; jumonji domain containing 1B; jumonji domain-containing protein 1B; jmjC domain-containing histone demethylation protein 2B
UniProt Protein Name
Lysine-specific demethylase 3B
UniProt Gene Name
KDM3B
UniProt Synonym Gene Names
C5orf7; JHDM2B; JMJD1B; KIAA1082
UniProt Entry Name
KDM3B_HUMAN
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Product Notes
The JMJD1B/KDM3B kdm3b (Catalog #AAA59678) is a Recombinant Protein produced from Baculovirus and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "JMJD1B/KDM3B, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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