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WB (Western Blot)
(Detection of human and mouse KAP-1 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-KAP-1 antibody AAA210855 (lot AAA210855-4) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.)
Rabbit anti-Human, Mouse KAP-1 Polyclonal Antibody | anti-KAP-1 antibody
Rabbit anti-KAP-1 Antibody, Affinity Purified
Gene Names
TRIM28; KAP1; TF1B; RNF96; TIF1B; PPP1R157
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
KAP-1, Antibody; Rabbit anti-KAP-1 Antibody, Affinity Purified; KRAB [Kruppel-associated box domain]-associated protein 1; RING finger protein 96; Tripartite motif-containing protein 28; transcriptional intermediary factor 1-beta; transcription intermediary factor 1-beta; TIF1-beta; TIF1B; TF1B; RNF96; RING-type E3 ubiquitin transferase TIF1-beta; protein phosphatase 1, regulatory subunit 157; PPP1R157; nuclear corepressor KAP-1; KRIP-1; KRAB-associated protein 1; KAP-1; KAP1; E3 SUMO-protein ligase TRIM28; KRAB-interacting protein 1; Transcription intermediary factor 1-beta; tripartite motif containing 28; anti-KAP-1 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-KAP-1 antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Rat
Immunogen
Between 650 and 700
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human and mouse KAP-1 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-KAP-1 antibody AAA210855 (lot AAA210855-4) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.)
WB (Western Blot)
(Detection of human and mouse KAP-1 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-KAP-1 antibody AAA210855 (lot AAA210855-4) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.)
IP (Immunoprecipitation)
(Detection of human KAP-1 by western blot of immunoprecipitates. Samples Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-KAP-1 antibody AAA210855 (lot AAA210855-4) used for IP at 6 ug per reaction. KAP-1 was also immunoprecipitated by a previous lot of this antibody (lot AAA210855-3) and rabbit anti-KAP-1 antibody For blotting immunoprecipitated KAP-1, AAA210855 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.)
IP (Immunoprecipitation)
(Detection of human KAP-1 by western blot of immunoprecipitates. Samples Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-KAP-1 antibody AAA210855 (lot AAA210855-4) used for IP at 6 ug per reaction. KAP-1 was also immunoprecipitated by a previous lot of this antibody (lot AAA210855-3) and rabbit anti-KAP-1 antibody For blotting immunoprecipitated KAP-1, AAA210855 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.)
IHC (Immunohiostchemistry)
(Detection of human Kap-1 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-Kap-1 Cat. No. AAA210855 Lot4 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB)
IHC (Immunohiostchemistry)
(Detection of human Kap-1 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-Kap-1 Cat. No. AAA210855 Lot4 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of mouse Kap-1 by immunohistochemistry. Sample: FFPE section of mouse plasmacytomaAntibody: Affinity purified rabbit anti-Kap-1 Cat. No. AAA210855 Lot4 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of mouse Kap-1 by immunohistochemistry. Sample: FFPE section of mouse plasmacytomaAntibody: Affinity purified rabbit anti-Kap-1 Cat. No. AAA210855 Lot4 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
Related Product Information for anti-KAP-1 antibody
KAP-1 (KRAB-Associated Protein 1) is a member of the tripartite motif RBCC/Trim domain family that contains a RING finger domain, B boxes, and an alpha helical coiled coil region. KAP-1 functions as a corepressor by interacting with the KRAB domain of KRAB zinc-finger transcriptional repressors. KAP-1 also appears to function as a scaffold for chromatin-remodeling complexes involved in transcriptional repression.
Product Categories/Family for anti-KAP-1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
79,474 Da
NCBI Official Full Name
Homo sapiens tripartite motif containing 28 (TRIM28), mRNA
NCBI Official Synonym Full Names
tripartite motif containing 28
NCBI Official Symbol
TRIM28
NCBI Official Synonym Symbols
KAP1; TF1B; RNF96; TIF1B; PPP1R157
NCBI Protein Information
transcription intermediary factor 1-beta; E3 SUMO-protein ligase TRIM28; KAP-1; KRAB [Kruppel-associated box domain]-associated protein 1; KRAB-associated protein 1; KRAB-interacting protein 1; KRIP-1; RING finger protein 96; TIF1-beta; nuclear corepresso
UniProt Protein Name
Transcription intermediary factor 1-beta
UniProt Gene Name
TRIM28
UniProt Synonym Gene Names
KAP1; RNF96; TIF1B; TIF1-beta; KAP-1; KRIP-1
UniProt Entry Name
TIF1B_HUMAN
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Product Notes
The KAP-1 trim28 (Catalog #AAA210855) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-KAP-1 Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's KAP-1 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the KAP-1 trim28 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "KAP-1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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