WB (Western Blot)
(Detection of mouse Phospho KAP-1 (S824) by western blot. Samples: Whole cell lysate (10 ug) from BeWo and AlphaTC1 Clone 9 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Phospho KAP-1 (S824) antibody AAA212519 (lot AAA212519-3) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.)
Rabbit anti-Mouse KAP-1 Polyclonal Antibody | anti-KAP-1 antibody
Rabbit anti-Phospho KAP-1 (S824) Antibody, Affinity Purified
Gene Names
Trim28; KAP-1; Tif1b; KRIP-1; MommeD9; AA408787; Tif1beta
Reactivity
Mouse
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
KAP-1, Antibody; Rabbit anti-Phospho KAP-1 (S824) Antibody, Affinity Purified; KAP-1, Phospho (S824); E3 SUMO-protein ligase TRIM28; Tif1beta; tripartite motif-containing protein 28; tripartite motif protein 28; transcriptional intermediary factor 1, beta; transcription intermediary factor 1-beta; TIF1-beta; RING-type E3 ubiquitin transferase TIF1-beta; MommeD9; KRIP-1; AA408787; KAP-1; Tif1b; KRAB-A-interacting protein; Transcription intermediary factor 1-beta; tripartite motif-containing 28; anti-KAP-1 antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-KAP-1 antibody
IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Rat
Immunogen
Surrounding serine 824
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of mouse Phospho KAP-1 (S824) by western blot. Samples: Whole cell lysate (10 ug) from BeWo and AlphaTC1 Clone 9 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Phospho KAP-1 (S824) antibody AAA212519 (lot AAA212519-3) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.)
WB (Western Blot)
(Detection of mouse Phospho KAP-1 (S824) by western blot. Samples: Whole cell lysate (10 ug) from BeWo and AlphaTC1 Clone 9 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Phospho KAP-1 (S824) antibody AAA212519 (lot AAA212519-3) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.)
IP (Immunoprecipitation)
(Detection of mouse Phospho KAP-1 (S824) by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from TCMK cells treated with etoposide (+) and/or Lambda PP (+) or mock treated (-). Antibodies: Affinity purified rabbit anti- KAP-1 antibody used for IP at 6 ug per reaction. For blotting immunoprecipitated Phospho KAP-1 (S824), AAA212519 lot 3 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second. Lower Panel: Rabbit anti-KAP-1 antibody .)
IP (Immunoprecipitation)
(Detection of mouse Phospho KAP-1 (S824) by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from TCMK cells treated with etoposide (+) and/or Lambda PP (+) or mock treated (-). Antibodies: Affinity purified rabbit anti- KAP-1 antibody used for IP at 6 ug per reaction. For blotting immunoprecipitated Phospho KAP-1 (S824), AAA212519 lot 3 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second. Lower Panel: Rabbit anti-KAP-1 antibody .)
Related Product Information for anti-KAP-1 antibody
KAP-1 (KRAB-Associated Protein 1) is a member of the tripartite motif RBCC/Trim domain family that contains a RING finger domain, B boxes, and an alpha helical coiled coil region. KAP-1 functions as a corepressor by interacting with the KRAB domain of KRAB zinc-finger transcriptional repressors. KAP-1 also appears to function as a scaffold for chromatin-remodeling complexes involved in transcriptional repression.
Product Categories/Family for anti-KAP-1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
88,847 Da
NCBI Official Full Name
transcription intermediary factor 1-beta
NCBI Official Synonym Full Names
tripartite motif-containing 28
NCBI Official Symbol
Trim28
NCBI Official Synonym Symbols
KAP-1; Tif1b; KRIP-1; MommeD9; AA408787; Tif1beta
NCBI Protein Information
transcription intermediary factor 1-beta; TIF1-beta; KRAB-A-interacting protein; tripartite motif protein 28; E3 SUMO-protein ligase TRIM28; tripartite motif-containing protein 28; transcriptional intermediary factor 1, beta
UniProt Protein Name
Transcription intermediary factor 1-beta
UniProt Gene Name
Trim28
UniProt Synonym Gene Names
Kap1; Krip1; Tif1b; TIF1-beta
UniProt Entry Name
TIF1B_MOUSE
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Product Notes
The KAP-1 trim28 (Catalog #AAA212519) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-Phospho KAP-1 (S824) Antibody, Affinity Purified reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's KAP-1 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the KAP-1 trim28 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "KAP-1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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