WB (Western Blot)
(Figure 1. Western blot analysis of KDM6B using anti-KDM6B antibody.)
anti-Human, Mouse KDM6B/JMJD3 Polyclonal Antibody | anti-KDM6B antibody
Anti-KDM6B/JMJD3 Antibody
Gene Names
KDM6B; JMJD3; NEDCFSA
Reactivity
Human, Mouse
Applications
ELISA, Flow Cytometry, Functional Assay, Western Blot
Purity
Immunogen affinity purified.
Synonyms
KDM6B/JMJD3, Antibody; Anti-KDM6B/JMJD3 Antibody; Lysine-specific demethylase 6B; JmjC domain-containing protein 3; Jumonji domain-containing protein 3; Lysine demethylase 6B; KDM6B; JMJD3; KIAA0346; anti-KDM6B antibody
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
No cross reactivity with other proteins.
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized; Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Sequence Length
1645
Applicable Applications for anti-KDM6B antibody
ELISA (Direct ELISA), FCM/FACS (Flow Cytometry), WB (Western Blot)
Immunogen
E Coli-derived human KDM6B/JMJD3 recombinant protein (Position: R1127-R1643).
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen store at -20 degree C for a longer time. Avoid repeated freezing and thawing.
WB (Western Blot)
(Figure 1. Western blot analysis of KDM6B using anti-KDM6B antibody.)
WB (Western Blot)
(Figure 1. Western blot analysis of KDM6B using anti-KDM6B antibody.)
FCM/FACS (Flow Cytometry)
FCM/FACS (Flow Cytometry)
FCM/FACS (Flow Cytometry)
FCM/FACS (Flow Cytometry)
Related Product Information for anti-KDM6B antibody
Description: Rabbit IgG polyclonal antibody for KDM6B/JMJD3 detection. Tested with WB, FCM, Direct ELISA in Human;Mouse.
Background: Lysine demethylase 6B is a protein that in humans is encoded by the KDM6B gene. It is mapped to 17p13.1. The protein encoded by this gene is a lysine-specific demethylase that specifically demethylates di- or tri-methylated lysine 27 of histone H3 (H3K27me2 or H3K27me3). H3K27 trimethylation is a repressive epigenetic mark controlling chromatin organization and gene silencing. This protein can also demethylate non-histone proteins such as retinoblastoma protein. Through its demethylation actvity this gene influences cellular differentiation and development, tumorigenesis, inflammatory diseases, and neurodegenerative diseases. This protein has two classical nuclear localization signals at its N-terminus. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
Background: Lysine demethylase 6B is a protein that in humans is encoded by the KDM6B gene. It is mapped to 17p13.1. The protein encoded by this gene is a lysine-specific demethylase that specifically demethylates di- or tri-methylated lysine 27 of histone H3 (H3K27me2 or H3K27me3). H3K27 trimethylation is a repressive epigenetic mark controlling chromatin organization and gene silencing. This protein can also demethylate non-histone proteins such as retinoblastoma protein. Through its demethylation actvity this gene influences cellular differentiation and development, tumorigenesis, inflammatory diseases, and neurodegenerative diseases. This protein has two classical nuclear localization signals at its N-terminus. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
References
1. Ge, C., Ye, J., Weber, C., Sun, W., Zhang, H., Zhou, Y., Cai, C., Qian, G., Capel, B. The histone demethylase KDM6B regulates temperature-dependent sex determination in a turtle species. Science 360: 645-648, 2018.
2. Sen, G. L., Webster, D. E., Barragan, D. I., Chang, H. Y., Khavari, P. A. Control of differentiation in a self-renewing mammalian tissue by the histone demethylase JMJD3. Genes Dev. 22: 1865-1870, 2008.
3. Stolerman, E. S., Francisco, E., Stallworth, J. L., Jones, J. R., Monaghan, K. G., Keller-Ramey, J., Person, R., Wentzensen, I. M., McWalter, K., Keren, B., Heron, B., Nava, C., and 19 others. Genetic variants in the KDM6B gene are associated with neurodevelopmental delays and dysmorphic features. Am. J. Med. Genet. 179A: 1276-1286, 2019.
2. Sen, G. L., Webster, D. E., Barragan, D. I., Chang, H. Y., Khavari, P. A. Control of differentiation in a self-renewing mammalian tissue by the histone demethylase JMJD3. Genes Dev. 22: 1865-1870, 2008.
3. Stolerman, E. S., Francisco, E., Stallworth, J. L., Jones, J. R., Monaghan, K. G., Keller-Ramey, J., Person, R., Wentzensen, I. M., McWalter, K., Keren, B., Heron, B., Nava, C., and 19 others. Genetic variants in the KDM6B gene are associated with neurodevelopmental delays and dysmorphic features. Am. J. Med. Genet. 179A: 1276-1286, 2019.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
hCG2036579, isoform CRA_a
NCBI Official Synonym Full Names
lysine demethylase 6B
NCBI Official Symbol
KDM6B
NCBI Official Synonym Symbols
JMJD3; NEDCFSA
NCBI Protein Information
lysine-specific demethylase 6B
UniProt Protein Name
Lysine-specific demethylase 6B
UniProt Gene Name
KDM6B
UniProt Synonym Gene Names
JMJD3; KIAA0346
UniProt Entry Name
KDM6B_HUMAN
Similar Products
Product Notes
The KDM6B kdm6b (Catalog #AAA125423) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-KDM6B/JMJD3 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's KDM6B/JMJD3 can be used in a range of immunoassay formats including, but not limited to, ELISA (Direct ELISA), FCM/FACS (Flow Cytometry), WB (Western Blot). Researchers should empirically determine the suitability of the KDM6B kdm6b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "KDM6B/JMJD3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.