IF (Immunofluorescence)
(Figure 8 Immunofluorescence Validation of JMJD3 in K562 Cells Immunofluorescent analysis of 4% paraformaldehyde fixed K562 cells labeling JMJD3 with AAA10925 at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).)
Rabbit JMJD3 Antibody | anti-KDM6B antibody
JMJD3 Antibody
The immunogen is located within amino acids 1370-1420 of JMJD3.
2)- K-562 Cell Slide
3) - K562 Cell Lysate
4)- A-20 Cell Lysate
IF (Immunofluorescence)
(Figure 8 Immunofluorescence Validation of JMJD3 in K562 Cells Immunofluorescent analysis of 4% paraformaldehyde fixed K562 cells labeling JMJD3 with AAA10925 at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).)
IF (Immunofluorescence)
(Figure 8 Immunofluorescence Validation of JMJD3 in K562 Cells Immunofluorescent analysis of 4% paraformaldehyde fixed K562 cells labeling JMJD3 with AAA10925 at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).)
ICC (Immunocytochemistry)
(Figure 7 Immunocytochemistry Validation of JMJD3 in K562 Cells Immunocytochemical analysis of K562 cells using anti- JMJD3 antibody (AAA10925) at 2.5 ug/ml. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.)
ICC (Immunocytochemistry)
(Figure 7 Immunocytochemistry Validation of JMJD3 in K562 Cells Immunocytochemical analysis of K562 cells using anti- JMJD3 antibody (AAA10925) at 2.5 ug/ml. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.)
WB (Western Blot)
(Figure 6 Western Blot Validation in HepG2 Cell LysateLoading: 15 ug of lysates per lane.Antibodies: JMJD3, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Lane 1: 0.25 ug/mL Lane 2: 0.5 ug/mL Lane 3: 0.25 ug/mL with blocking peptide)
WB (Western Blot)
(Figure 6 Western Blot Validation in HepG2 Cell LysateLoading: 15 ug of lysates per lane.Antibodies: JMJD3, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Lane 1: 0.25 ug/mL Lane 2: 0.5 ug/mL Lane 3: 0.25 ug/mL with blocking peptide)
WB (Western Blot)
(Figure 5 Western Blot Validation in Mouse A-20 Cell LysateLoading: 15 ug of lysates per lane.Antibodies: JMJD3, (0.5 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Lane 1: in the absence and Lane 2: in the presence of blocking peptide)
WB (Western Blot)
(Figure 5 Western Blot Validation in Mouse A-20 Cell LysateLoading: 15 ug of lysates per lane.Antibodies: JMJD3, (0.5 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Lane 1: in the absence and Lane 2: in the presence of blocking peptide)
WB (Western Blot)
(Figure 4 Western Blot Validation in K562 Cell LysateLoading: 15 ug of lysates per lane.Antibodies: : JMJD3, (0.5 ug/mL) , 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Lane 1: in the absence and Lane 2: in the presence of blocking peptide)
WB (Western Blot)
(Figure 4 Western Blot Validation in K562 Cell LysateLoading: 15 ug of lysates per lane.Antibodies: : JMJD3, (0.5 ug/mL) , 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Lane 1: in the absence and Lane 2: in the presence of blocking peptide)
WB (Western Blot)
(Figure 3 Western Blot Validation in HeLa Cell LysateLoading: 15 ug of lysates per lane.Antibodies: JMJD3, (0.25 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.)
WB (Western Blot)
(Figure 3 Western Blot Validation in HeLa Cell LysateLoading: 15 ug of lysates per lane.Antibodies: JMJD3, (0.25 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.)
WB (Western Blot)
(Figure 2 Independent Antibody Validation (IAV) via Protein Expression Profile in Cell LinesLoading: 15 ug of lysates per lane.Antibodies: JMJD3 (1 ug/mL), JMJD3 14-618, (2 ug/mL) and beta-actin 3779 (1ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.)
WB (Western Blot)
(Figure 2 Independent Antibody Validation (IAV) via Protein Expression Profile in Cell LinesLoading: 15 ug of lysates per lane.Antibodies: JMJD3 (1 ug/mL), JMJD3 14-618, (2 ug/mL) and beta-actin 3779 (1ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.)
WB (Western Blot)
(Figure 1 Western Blot Validation in Human, Mouse and Rat Cell LinesLoading: 15 ug of lysates per lane.Antibodies: JMJD3, (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution)
WB (Western Blot)
(Figure 1 Western Blot Validation in Human, Mouse and Rat Cell LinesLoading: 15 ug of lysates per lane.Antibodies: JMJD3, (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution)
NCBI and Uniprot Product Information
Observed: 180 kD
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Product Notes
The KDM6B kdm6b (Catalog #AAA10925) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The JMJD3 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's JMJD3 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blot (WB). Optimal dilutions for each application to be determined by the researcher. Researchers should empirically determine the suitability of the KDM6B kdm6b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "JMJD3, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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