Rabbit KEAP1 Polyclonal Antibody | anti-KEAP1 antibody

KEAP1 Antibody

Cat. Num. AAA170589

• Gene Names: KEAP1; INrf2; KLHL19
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Western Blot
• Purity: Antigen affinity purification
• Synonyms: KEAP1, Antibody; KEAP1 Antibody; INrf2; KIAA0132; KLHL19; MGC10630; MGC1114; MGC20887; MGC4407; MGC9454; anti-KEAP1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Antigen affinity purification
• Concentration: 1mg/mL (varies by lot)
• Sequence Length: 624

• Applicable Applications for anti-KEAP1 antibody: IHC (Immunohistochemistry), WB (Western Blot)
• Immunogen: Recombinant protein of human KEAP1
• Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3
• Preparation and Storage: Store at -20 degree C (regular) and -80 degree C (long term). Avoid freeze / thaw cycles.

IHC (Immunohiostchemistry)

(Immunohistochemistry of paraffin-embedded rat spleen using KEAP1 antibody at dilution of 1:200 (x400 lens))

WB (Western Blot)

(Western blot analysis of extracts of HepG2 cell lines, using KEAP1 antibody.)

Related Product Information for anti-KEAP1 antibody

The nuclear factor-like 2 (NRF2) transcriptional activator binds antioxidant response elements (ARE) of target gene promoter regions to regulate expression of oxidative stress response genes. Under basal conditions, the NRF2 inhibitor INrf2 (also called KEAP1) binds and retains NRF2 in the cytoplasm where it can be targeted for ubiquitin-mediated degradation (1). Small amounts of constitutive nuclear NRF2 maintains cellular homeostasis through regulation of basal expression of antioxidant response genes. Following oxidative or electrophilic stress, KEAP1 releases NRF2, thereby allowing the activator to translocate to the nucleus and bind to ARE-containing genes (2). The coordinated action of NRF2 and other transcription factors mediates the response to oxidative stress (3). Altered expression of NRF2 is associated with chronic obstructive pulmonary disease (COPD) (4). NRF2 activity in lung cancer cell lines directly correlates with cell proliferation rates, and inhibition of NRF2 expression by siRNA enhances anti-cancer drug-induced apoptosis (5). The NRF2 repressor KEAP1 contains an amino terminal BTB/POZ domain and a carboxyl terminal KELCH domain (6, 7). The KELCH domain is required for interacting with NRF2 and the BTB/POZ domain functions in binding Cul3 E3 ubiquitin ligase (8-10). Under normal conditions, the complex leads to the cytoplasmic sequestration and ubiquitin-mediated proteasomal degradation of NRF2. Electrophilic modification of KEAP1 leads to disassociation of the NRF2/KEAP1 complex. KEAP1 also targets the down regulation of NF-kappaB activity by targeting IKKbeta degradation (11).

NCBI and Uniprot Product Information

• NCBI GI #: 45269145
• NCBI GeneID: 9817
• NCBI Accession #: NP_987096.1
• NCBI GenBank Nucleotide #: NM_203500.1
• Molecular Weight: 69,666 Da
• NCBI Official Full Name: kelch-like ECH-associated protein 1
• NCBI Official Synonym Full Names: kelch-like ECH-associated protein 1
• NCBI Official Symbol: KEAP1
• NCBI Official Synonym Symbols: INrf2; KLHL19
• NCBI Protein Information: kelch-like ECH-associated protein 1; cytosolic inhibitor of Nrf2; kelch-like family member 19; kelch-like protein 19
• UniProt Protein Name: Kelch-like ECH-associated protein 1
• UniProt Gene Name: KEAP1
• UniProt Synonym Gene Names: INRF2; KIAA0132; KLHL19; INrf2
• UniProt Entry Name: KEAP1_HUMAN