WB (Western Blot)
(Detection of human and mouse Kindlin-2 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Kindlin-2 antibody AAA212783 (lot AAA212783-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Rabbit anti-Human, Mouse Kindlin-2 Polyclonal Antibody | anti-FERMT2 antibody
Rabbit anti-Kindlin-2 Antibody, Affinity Purified
Reactivity
Human, Mouse
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
Kindlin-2, Antibody; Rabbit anti-Kindlin-2 Antibody, Affinity Purified; mitogen inducible gene 2 protein; UNC112; PLEKHC1; Pleckstrin homology domain-containing family C member 1; pleckstrin homology domain containing, family C member 1; pleckstrin homology domain containing, family C (with FERM domain) member 1; UNC112B; mig-2; MIG2; Kindlin-2; kindlin 2; KIND2; fermitin family homolog 2; PH domain-containing family C member 1; Mitogen-inducible gene 2 protein; Fermitin family homolog 2; fermitin family member 2; anti-FERMT2 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-FERMT2 antibody
IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 175 and 225
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human and mouse Kindlin-2 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Kindlin-2 antibody AAA212783 (lot AAA212783-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
WB (Western Blot)
(Detection of human and mouse Kindlin-2 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Kindlin-2 antibody AAA212783 (lot AAA212783-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human Kindlin-2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Kindlin-2 antibody AAA212783 (lot AAA212783-1) used for IP at 6 ug per reaction. Kindlin-2 was also immunoprecipitated by rabbit anti-Kindlin-2 antibody BL17335. For blotting immunoprecipitated Kindlin-2, AAA212783 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human Kindlin-2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Kindlin-2 antibody AAA212783 (lot AAA212783-1) used for IP at 6 ug per reaction. Kindlin-2 was also immunoprecipitated by rabbit anti-Kindlin-2 antibody BL17335. For blotting immunoprecipitated Kindlin-2, AAA212783 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Related Product Information for anti-FERMT2 antibody
Kindlin-2 is a scaffolding protein that enhances integrin activation mediated by TLN1 and/or TLN2, but activates integrins only weakly by itself. It binds to membranes enriched in phosphoinositides. It also enhances integrin-mediated cell adhesion onto the extracellular matrix and cell spreading; this requires both its ability to interact with integrins and with phospholipid membranes. Kindlin-2 is required for the assembly of focal adhesions and participates in the connection between extracellular matrix adhesion sites and the actin cytoskeleton and also in the orchestration of actin assembly and cell shape modulation. Kindlin-2 recruits FBLIM1 to focal adhesions. It plays a role in the TGFB1 and integrin signaling pathways, and it stabilizes active CTNNB1 and plays a role in the regulation of transcription mediated by CTNNB1 and TCF7L2/TCF4 and in Wnt signaling [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q96AC1].
Product Categories/Family for anti-FERMT2 antibody
NCBI and Uniprot Product Information
Similar Products
Product Notes
The FERMT2 fermt2 (Catalog #AAA212783) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-Kindlin-2 Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Kindlin-2 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the FERMT2 fermt2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Kindlin-2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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