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product-image-AAA288562_FCM13.jpg FCM/FACS (Flow Cytometry) (KLF14 Antibody (C-term) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Rabbit anti-Human, mouse KLF14 Polyclonal Antibody | anti-KLF14 antibody

KLF14 Antibody (C-term)

Reactivity
Human, mouse
Applications
Flow Cytometry, Functional Assay, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
KLF14, Antibody; KLF14 Antibody (C-term); Krueppel-like factor 14; Basic transcription element-binding protein 5; BTE-binding protein 5; Transcription factor BTEB5; KLF14; BTEB5; anti-KLF14 antibody
Ordering
Host
Rabbit
Reactivity
Human, mouse
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This KLF14 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 254-283 amino acids from the C-terminal region of human KLF14.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
254-283
Sequence Length
323
Applicable Applications for anti-KLF14 antibody
FCM/FACS (Flow Cytometry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

FCM/FACS (Flow Cytometry)

(KLF14 Antibody (C-term) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

product-image-AAA288562_FCM13.jpg FCM/FACS (Flow Cytometry) (KLF14 Antibody (C-term) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

WB (Western Blot)

(KLF14 Antibody (C-term) western blot analysis in mouse kidney tissue lysates (35ug/lane).This demonstrates the KLF14 antibody detected the KLF14 protein (arrow).)

product-image-AAA288562_WB15.jpg WB (Western Blot) (KLF14 Antibody (C-term) western blot analysis in mouse kidney tissue lysates (35ug/lane).This demonstrates the KLF14 antibody detected the KLF14 protein (arrow).)
Related Product Information for anti-KLF14 antibody
This gene encodes a member of the Kruppel-like family of transcription factors. The gene exhibits imprinted expression from the maternal allele in embryonic and extra-embryonic tissues. Expression of this gene is induced by TGF-beta, and the protein represses TGF-beta receptor II expression. The protein functions as part of a transcriptional co-repressor complex that also contains the transcriptional regulator SIN3A and histone deacetylase 2.
References
Voight, B.F., et al. Nat. Genet. 42(7):579-589(2010) Chasman, D.I., et al. PLoS Genet. 5 (11), E1000730 (2009) : Stacey, S.N., et al. Nat. Genet. 41(8):909-914(2009) Truty, M.J., et al. J. Biol. Chem. 284(10):6291-6300(2009) Parker-Katiraee, L., et al. PLoS Genet. 3 (5), E65 (2007) :

NCBI and Uniprot Product Information

NCBI GI #
UniProt Accession #
Molecular Weight
33124
NCBI Official Full Name
Krueppel-like factor 14
UniProt Protein Name
Krueppel-like factor 14
UniProt Gene Name
KLF14
UniProt Synonym Gene Names
BTEB5; BTE-binding protein 5
UniProt Entry Name
KLF14_HUMAN

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Product Notes

The KLF14 klf14 (Catalog #AAA288562) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 254-283. The KLF14 Antibody (C-term) reacts with Human, mouse and may cross-react with other species as described in the data sheet. AAA Biotech's KLF14 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the KLF14 klf14 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "KLF14, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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