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WB (Western Blot)
(CYK18 Antibody (C-term) Western blot of A431 cell line lysates (35 ug/lane). This demonstrates the CYK18 antibody detected the CYK18 protein (arrow) (Kindly offered by Dr. Li).)
Rabbit anti-Human, Mouse KRT18 / CK18 / Cytokeratin 18 Polyclonal Antibody | anti-KRT18 antibody
Rabbit Polyclonal to Human KRT18 / CK18 / Cytokeratin 18
Gene Names
KRT18; K18; CYK18
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Immunofluorescence, Western Blot, Flow Cytometry, Functional Assay
Purity
Ammonium sulfate precipitation
Synonyms
KRT18 / CK18 / Cytokeratin 18; Polyclonal Antibody; Rabbit Polyclonal to Human KRT18 / CK18 / Cytokeratin 18; Anti-KRT18 / CK18 / Cytokeratin 18 Antibody (C-Terminus) IHC-plus; KRT18; CK-18; Cytokeratin 18; Cytokeratin-18; K18; Keratin 18; CYK18; Keratin-18; Human KRT18; CK18; anti-KRT18 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Ammonium sulfate precipitation
Form/Format
PBS, 0.09% sodium azide
Sequence Length
430
Applicable Applications for anti-KRT18 antibody
Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), Flow Cytometry (FC/FACS)
Application Notes
Flo (1:10 - 1:50), IF (1:10 - 1:50), IHC-P (5 ug/ml), WB (1:1000)
Target Species
Human
Immunogen Description
KLH conjugated synthetic peptide selected from the C-terminal region of human CYK18.
Immunogen Type
Synthetic peptide
Immunogen
KRT18 / CK18 / Cytokeratin 18 antibody was raised against kLH conjugated synthetic peptide selected from the C-terminal region of human CYK18.
Antigen Modification
C-Terminus
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.
WB (Western Blot)
(CYK18 Antibody (C-term) Western blot of A431 cell line lysates (35 ug/lane). This demonstrates the CYK18 antibody detected the CYK18 protein (arrow) (Kindly offered by Dr. Li).)
WB (Western Blot)
(CYK18 Antibody (C-term) Western blot of A431 cell line lysates (35 ug/lane). This demonstrates the CYK18 antibody detected the CYK18 protein (arrow) (Kindly offered by Dr. Li).)
WB (Western Blot)
(Western blot of CYK18 Antibody (C-term) in K562, NCI-H460 cell line lysates and mouse stomach tissues lysates(35 ug/lane). CYK18(arrow) was detected using the purified Pab.)
WB (Western Blot)
(Western blot of CYK18 Antibody (C-term) in K562, NCI-H460 cell line lysates and mouse stomach tissues lysates(35 ug/lane). CYK18(arrow) was detected using the purified Pab.)
FCM (Flow Cytometry)
(Flow cytometry of WiDr cells using CYK18 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM (Flow Cytometry)
(Flow cytometry of WiDr cells using CYK18 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM (Flow Cytometry)
(Formalin-fixed and paraffin-embedded human colon carcinoma with CYK18 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.)
FCM (Flow Cytometry)
(Formalin-fixed and paraffin-embedded human colon carcinoma with CYK18 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.)
IF (Immunofluorescence)
(Confocal immunofluorescence of CYK18 Antibody (C-term) with prostate carcinoma followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
IF (Immunofluorescence)
(Confocal immunofluorescence of CYK18 Antibody (C-term) with prostate carcinoma followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of CYK18 Antibody (C-term) with HeLa cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).)
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of CYK18 Antibody (C-term) with HeLa cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).)
IHC (Immunohistochemistry)
(Anti-KRT18 / CK18 / Cytokeratin 18 antibody IHC staining of human prostate, epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)
IHC (Immunohistochemistry)
(Anti-KRT18 / CK18 / Cytokeratin 18 antibody IHC staining of human prostate, epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)
Related Product Information for anti-KRT18 antibody
KRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis.
Product Categories/Family for anti-KRT18 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
48,058 Da
NCBI Official Full Name
keratin, type I cytoskeletal 18
NCBI Official Synonym Full Names
keratin 18
NCBI Official Symbol
KRT18
NCBI Official Synonym Symbols
K18; CYK18
NCBI Protein Information
keratin, type I cytoskeletal 18; CK-18; keratin-18; cytokeratin 18; cytokeratin-18; cell proliferation-inducing protein 46; cell proliferation-inducing gene 46 protein
UniProt Protein Name
Keratin, type I cytoskeletal 18
UniProt Gene Name
KRT18
UniProt Synonym Gene Names
CYK18; CK-18; K18
UniProt Entry Name
K1C18_HUMAN
Similar Products
Product Notes
The KRT18 krt18 (Catalog #AAA12370) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit Polyclonal to Human KRT18 / CK18 / Cytokeratin 18 reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's KRT18 / CK18 / Cytokeratin 18 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), Flow Cytometry (FC/FACS). Flo (1:10 - 1:50), IF (1:10 - 1:50), IHC-P (5 ug/ml), WB (1:1000). Researchers should empirically determine the suitability of the KRT18 krt18 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "KRT18 / CK18 / Cytokeratin 18, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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