Rabbit LCK Polyclonal Antibody | anti-LCK antibody

LCK Antibody

Cat. Num. AAA168769

• Gene Names: LCK; LSK; YT16; IMD22; p56lck; pp58lck
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Western Blot
• Purity: Antigen affinity purification
• Synonyms: LCK, Antibody; LCK Antibody; YT16; p56lck; pp58lck; anti-LCK antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Antigen affinity purification
• Concentration: 1mg/mL (varies by lot)
• Sequence Length: 35

• Applicable Applications for anti-LCK antibody: IHC (Immunohistochemistry), WB (Western Blot)
• Immunogen: Recombinant protein of human LCK
• Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3
• Santa Cruz Alternative: Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-32014 / sc-13 / sc-28882
• Preparation and Storage: Store at -20 degree C (regular) and -80 degree C (long term). Avoid freeze / thaw cycles.

IHC (Immunohistochemisry)

(Immunohistochemical of paraffin-embedded human kidney cancer using LCK antibody at dilution of 1:100 (400x lens).)

IHC (Immunohiostchemistry)

(Immunohistochemistry of paraffin-embedded human kidney using LCK antibody at dilution of 1:100 (400x lens).)

WB (Western Blot)

(Western blot analysis of extracts of various cell lines, using LCK antibody.)

Related Product Information for anti-LCK antibody

The Src family of protein tyrosine kinases, which includes Src, Lyn, Fyn, Yes, Lck, Blk, and Hck, are important in the regulation of growth and differentiation of eukaryotic cells (1). Src activity is regulated by tyrosine phosphorylation at two sites, but with opposing effects. While phosphorylation at Tyr416 in the activation loop of the kinase domain upregulates enzyme activity, phosphorylation at Tyr527 in the carboxy-terminal tail by Csk renders the enzyme less active (2). p56lck (lymphocyte cellular kinase) has been shown to be a pivotal enzyme to both maturation of thymocytes and activation and proliferation of peripheral lymphocytes. The p56lck sequence appeared highly homologous to that of the oncogene p60c-src as did its exon-intron organisation. p56lck does not appear involved in lymphoproliferative diseases, either by overexpression or activating mutations. Nevertheless, its aberrant expression has been reported in some carcinomas (colon, lung and mammary). It was suggested that p56lck could favor metastases by facilitating loss of cell adhesion (3).

NCBI and Uniprot Product Information

• NCBI GI #: 349702
• NCBI GeneID: 3932
• Molecular Weight: 61,190 Da
• NCBI Official Full Name: LCK, partial
• NCBI Official Synonym Full Names: LCK proto-oncogene, Src family tyrosine kinase
• NCBI Official Symbol: LCK
• NCBI Official Synonym Symbols: LSK; YT16; IMD22; p56lck; pp58lck
• NCBI Protein Information: tyrosine-protein kinase Lck; T-lymphocyte specific protein tyrosine kinase p56lck; leukocyte C-terminal Src kinase; lymphocyte cell-specific protein-tyrosine kinase; lymphocyte-specific protein tyrosine kinase; p56(LSTRA) protein-tyrosine kinase; proto-oncogene tyrosine-protein kinase LCK; t cell-specific protein-tyrosine kinase
• UniProt Protein Name: Tyrosine-protein kinase Lck
• UniProt Gene Name: LCK
• UniProt Synonym Gene Names: LSK
• UniProt Entry Name: LCK_HUMAN