Rabbit anti-Human Lck Polyclonal Antibody | anti-Lck antibody

Anti-Lck Antibody, Rabbit Polyclonal

Cat. Num. AAA254234

• Gene Names: LCK; LSK; YT16; p56lck; pp58lck
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Protein A & Antigen Affinity
• Synonyms: Lck, Antibody; Anti-Lck Antibody, Rabbit Polyclonal; Lck Antibody, Rabbit PAb, Antigen Affinity Purified; LCK proto-oncogene, Src family tyrosine kinase; Anti-IMD22 Antibody; Anti-LSK Antibody; Anti-p56lck Antibody; Anti-pp58lck Antibody; Anti-YT16 Antibody; anti-Lck antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human Lck
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-Lck antibody: IHC (Immunohistochemistry), ELISA, WB (Western Blot)
• Immunogen: Recombinant Human Lck Protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Human Lck (rh Lck). Lck specific IgG was purified by Human Lck affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

IHC (Immunohistochemisry)

(Immunochemical staining of human LCK in human lymph node with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).)

IHC (Immunohiostchemistry)

(Immunochemical staining of human LCK in human tonsil with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).)

WB (Western Blot)

(Anti-LCK rabbit polyclonal antibody at 1:500 dilutionLane A: Jurkat Whole Cell LysateLane B: MOLT4 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit  IgG H&L (Dylight 800)  at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:58 kDaObserved band size:58 kDa)

Related Product Information for anti-Lck antibody

Protein kinases are critically involved in signaling pathways that regulate cell growth, differentiation, activation, and survival. Initially identified as a T-cell specific member of the Src family of protein tyrosine kinases, Lck has become the object of intensive investigations which have revealed a key role for this kinase in the central processes controlling T-cell development, activation, proliferation, and survival. Lck is expressed specifically in lymphoid cells. It contains one protein kinase domain, one SH2 domain, and one SH3 domain. It is associated with a variety of cell surface receptors and is critical for signal transduction from the T-cell antigen receptor (TCR). Consequently, Lck is targeted by regulatory proteins of T-lymphotropic viruses, especially by the Herpesvirus saimiri (HVS) tyrosine kinase interacting protein (Tip). This oncoprotein physically interacts with Lck in HVS transformed T cells and has an impact on its catalytic activity. Together with the identification of defects in the regulation of Lck expression or activity in T-cell leukemias, suggests that dysregulation of Lck might play a role in neoplastic transformation. However, under certain conditions, Lck is also involved in the induction of apoptosis. This chemosensitizing effect of Lck is independent of T-cell receptor signaling and does not require the kinase activity of Lck. The findings demonstrate that Lck might be part of two independent signaling pathways leading to either cell proliferation or apoptosis.

Product Categories/Family for anti-Lck antibody

NF-kB (NFkB) Pathway

References

Majolini MB, et al. (1999) Dysregulation of the protein tyrosine kinase LCK in lymphoproliferative disorders and in other neoplasias. Leuk Lymphoma. 35(3-4): 245-54.Isakov N, et al. (2000) Lck protein tyrosine kinase is a key regulator of T-cell activation and a target for signal intervention by Herpesvirus saimiri and other viral gene products. Eur J Biochem. 267(12): 3413-21.Heyninck K, et al. (2006) A novel link between Lck, Bak expression and chemosensitivity. Oncogene. 25(12): 1693-5.

NCBI and Uniprot Product Information

• NCBI GI #: 112789548
• NCBI GeneID: 3932
• NCBI Accession #: NP_001036236.1
• NCBI GenBank Nucleotide #: NM_001042771.1
• UniProt Accession #: P06239; P07100; Q12850; Q13152; Q5TDH8; Q5TDH9; Q7RTZ3; Q96DW4; Q9NYT8; D3DPP8
• Molecular Weight: 58,001 Da
• NCBI Official Full Name: tyrosine-protein kinase Lck
• NCBI Official Synonym Full Names: lymphocyte-specific protein tyrosine kinase
• NCBI Official Symbol: LCK
• NCBI Official Synonym Symbols: LSK; YT16; p56lck; pp58lck
• NCBI Protein Information: tyrosine-protein kinase Lck; leukocyte C-terminal Src kinase; p56(LSTRA) protein-tyrosine kinase; t cell-specific protein-tyrosine kinase; proto-oncogene tyrosine-protein kinase LCK; lymphocyte cell-specific protein-tyrosine kinase; T-lymphocyte specific
• UniProt Protein Name: Tyrosine-protein kinase Lck
• UniProt Gene Name: LCK
• UniProt Synonym Gene Names: LSK
• UniProt Entry Name: LCK_HUMAN