Rabbit anti-Human Lck Polyclonal Antibody | anti-Lck antibody

Lck (phospho-Tyr505) Antibody

Cat. Num. AAA301453

• Gene Names: LCK; LSK; YT16; IMD22; p56lck; pp58lck
• Reactivity: Human
• Applications: Western Blot
• Purity: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
• Synonyms: Lck, Antibody; Lck (phospho-Tyr505) Antibody; Lymphocyte cell-specific protein-tyrosine kinase; p56-LCK; LSK; T cell-specific protein-tyrosine kinase; anti-Lck antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous level of Lck only when phosphorylated at tyrosine 505.
• Purity/Purification: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
• Form/Format: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 509

• Applicable Applications for anti-Lck antibody: WB (Western Blot)
• Immunogen Type: Peptide-KLH
• Immunogen Description: Peptide sequence around phosphorylation site of tyrosine 505 (G-Q-Y(p)-Q-P) derived from Human Lck.
• Modification: Phospho-Tyr505
• Target Name: Lck
• Preparation and Storage: Store at -20 degree C for long term preservation (recommended). Store at 4 degree C for short term use.

WB (Western Blot)

(Western blot analysis of extracts from 293 cells, treated with calf intestinal phosphatase (CIP), using Lck (phospho-Tyr505) Antibody.)

WB (Western Blot)

(Western blot analysis of extracts from JK cells using Lck(phospho-Tyr505) Antibody and the same antibody preincubated with blocking peptide.)

Related Product Information for anti-Lck antibody

Tyrosine kinase that plays an essential role for the selection and maturation of developing T-cell in the thymus and in mature T-cell function. Is constitutively associated with the cytoplasmic portions of the CD4 and CD8 surface receptors and plays a key role in T-cell antigen receptor(TCR)-linked signal transduction pathways. Association of the TCR with a peptide antigen-bound MHC complex facilitates the interaction of CD4 and CD8 with MHC class II and class I molecules, respectively, and thereby recruits the associated LCK to the vicinity of the TCR/CD3 complex. LCK then phosphorylates tyrosines residues within the immunoreceptor tyrosines-based activation motifs (ITAMs) in the cytoplasmic tails of the TCRgamma chains and CD3 subunits, initiating the TCR/CD3 signaling pathway. In addition, contributes to signaling by other receptor molecules. Associates directly with the cytoplasmic tail of CD2, and upon engagement of the CD2 molecule, LCK undergoes hyperphosphorylation and activation. Also plays a role in the IL2 receptor-linked signaling pathway that controls T-cell proliferative response. Binding of IL2 to its receptor results in increased activity of LCK. Is expressed at all stages of thymocyte development and is required for the regulation of maturation events that are governed by both pre-TCR and mature a beta TCR.

Product Categories/Family for anti-Lck antibody

Phospho-specific Ab

NCBI and Uniprot Product Information

• NCBI GI #: 112789548
• NCBI GeneID: 3932
• NCBI Accession #: NP_001036236.1
• NCBI GenBank Nucleotide #: NM_001042771.2
• UniProt Accession #: P06239; P07100; Q12850; Q13152; Q5TDH8; Q5TDH9; Q7RTZ3; Q96DW4; Q9NYT8; D3DPP8
• Molecular Weight: 61,190 Da
• NCBI Official Full Name: tyrosine-protein kinase Lck
• NCBI Official Synonym Full Names: LCK proto-oncogene, Src family tyrosine kinase
• NCBI Official Symbol: LCK
• NCBI Official Synonym Symbols: LSK; YT16; IMD22; p56lck; pp58lck
• NCBI Protein Information: tyrosine-protein kinase Lck
• UniProt Protein Name: Tyrosine-protein kinase Lck
• UniProt Gene Name: LCK
• UniProt Synonym Gene Names: LSK
• UniProt Entry Name: LCK_HUMAN