WB (Western Blot)
(Detection of human LCP2 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-LCP2 antibody AAA213172 (lot AAA213172-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Rabbit anti-Human LCP2/SLP76 Polyclonal Antibody | anti-LCP2/SLP76 antibody
Rabbit anti-LCP2/SLP76 Antibody, Affinity Purified
Gene Names
LCP2; SLP76; SLP-76
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
LCP2/SLP76, Antibody; Rabbit anti-LCP2/SLP76 Antibody, Affinity Purified; 76 kDa tyrosine phosphoprotein; SLP-76 tyrosine phosphoprotein; SLP-76; SLP76; lymphocyte cytosolic protein 2; SH2 domain-containing leukocyte protein of 76 kDa; Lymphocyte cytosolic protein 2; anti-LCP2/SLP76 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-LCP2/SLP76 antibody
IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 225 and 275
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human LCP2 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-LCP2 antibody AAA213172 (lot AAA213172-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
WB (Western Blot)
(Detection of human LCP2 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-LCP2 antibody AAA213172 (lot AAA213172-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human LCP2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-LCP2 antibody AAA213172 (lot AAA213172-1) used for IP at 6 ug per reaction. LCP2 was also immunoprecipitated by rabbit anti-LCP2 antibody For blotting immunoprecipitated LCP2, A305-243 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human LCP2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-LCP2 antibody AAA213172 (lot AAA213172-1) used for IP at 6 ug per reaction. LCP2 was also immunoprecipitated by rabbit anti-LCP2 antibody For blotting immunoprecipitated LCP2, A305-243 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Related Product Information for anti-LCP2/SLP76 antibody
Lymphocyte cytosolic protein 2 (LCP2) was originally identified as a substrate of the ZAP-70 protein tyrosine kinase following T cell receptor (TCR) ligation in the leukemic T cell line Jurkat. The SLP-76 locus has been localized to human chromosome 5q33 and the gene structure has been partially characterized in mice. The human and murine cDNAs both encode 533 amino acid proteins that are 72% identical and comprised of three modular domains. The NH2-terminus contains an acidic region that includes a PEST domain and several tyrosine residues which are phosphorylated following TCR ligation. LCP2 also contains a central proline-rich domain and a COOH-terminal SH2 domain. A number of additional proteins have been identified that associate with LCP2 both constitutively and inducibly following receptor ligation, supporting the notion that LCP2 functions as an adaptor or scaffold protein. Studies using SLP-76 deficient T cell lines or mice have provided strong evidence that LCP2 plays a positive role in promoting T cell development and activation as well as mast cell and platelet function [taken from NCBI Entrez Gene (Gene ID: 3937)].
Product Categories/Family for anti-LCP2/SLP76 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
533
NCBI Official Full Name
lymphocyte cytosolic protein 2
NCBI Official Synonym Full Names
lymphocyte cytosolic protein 2 (SH2 domain containing leukocyte protein of 76kDa)
NCBI Official Symbol
LCP2
NCBI Official Synonym Symbols
SLP76; SLP-76
NCBI Protein Information
lymphocyte cytosolic protein 2; 76 kDa tyrosine phosphoprotein; SLP-76 tyrosine phosphoprotein; SH2 domain-containing leukocyte protein of 76kD; SH2 domain-containing leukocyte protein of 76 kDa
UniProt Protein Name
Lymphocyte cytosolic protein 2
UniProt Gene Name
LCP2
UniProt Synonym Gene Names
SLP76
UniProt Entry Name
LCP2_HUMAN
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Product Notes
The LCP2/SLP76 lcp2 (Catalog #AAA213172) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-LCP2/SLP76 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's LCP2/SLP76 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the LCP2/SLP76 lcp2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LCP2/SLP76, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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