anti-Human CD2 Antibody | anti-CD2 antibody

Anti-Hu CD2 APC

Cat. Num. AAA129283

• Gene Names: CD2; T11; SRBC; LFA-2
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay
• Synonyms: CD2; N/A; Anti-Hu CD2 APC; CD2 molecule; T11; LFA-2; SRBC; anti-CD2 antibody

• Reactivity: Human
• Isotype: Mouse IgG1 kappa
• Clone Number: TS1/8
• Specificity: The mouse monoclonal antibody TS1/8 recognizes an extracellular epitope of CD2, a 50 kDa glycoprotein present on the human peripheral blood T lymphocytes and NK cells; also expressed by all thymocytes.
• Form/Format: APC; Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.; Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 m

• Applicable Applications for anti-CD2 antibody: FCM/FACS (Flow Cytometry)
• Immunogen: Cytotoxic T lymphocytes
• Preparation and Storage: Store at 2-8 degree C. Protect from prolonged exposure to light. Do not freeze.

FCM/FACS (Flow Cytometry)

(Separation of human CD2 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD2 (TS1/8) APC antibody (10 ?l reagent / 100 ?l of peripheral whole blood).)

FCM/FACS (Flow Cytometry)

(Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD2 (TS1/8) APC antibody (10 ?l reagent / 100 ?l of peripheral whole blood).)

Related Product Information for anti-CD2 antibody

CD2 belongs to T lymphocyte glycoproteins of immunoglobulin superfamily. Its interaction with CD58 stabilizes adhesion between T cells and antigen presenting or target cells. Relatively low affinity of CD2 to CD58 (as measured in solution) is compensated within the two-dimensional cell-cell interface to provide tight adhesion. Moreover, T cell activation induces increased CD2 expression and its lateral mobility, making easier contact between CD2 and CD58. Subsequently, T cell activation causes fixation of CD58-CD2 at sites of cell-cell contact, thereby strengthening intercellular adhesion. CD2 deficiency reduces intestinal inflammation and helps to control infection.

References

*Sanchez-Madrid F, Krensky AM, Ware CF, Robbins E, Strominger JL, Burakoff SJ, Springer TA: Three distinct antigens associated with human T-lymphocyte-mediated cytolysis: LFA-1, LFA-2, and LFA-3. Proc Natl Acad Sci U S A. 1982 Dec;79(23):7489-93., URL: http://www.ncbi.nlm.nih.gov/pubmed/6984191 Vollger,*Zhang B, Dai M, Li QJ, Zhuang Y: Tracking proliferative history in lymphocyte development with cre-mediated sister chromatid recombination. PLoS Genet. 2013 Oct;9(10):e1003887., URL: http://www.ncbi.nlm.nih.gov/pubmed/24204301,
*Pawlowski NN, Struck D, Grollich K, Kuhl AA, Zeitz M, Liesenfeld O, Hoffmann JC: CD2 deficiency partially prevents small bowel inflammation and improves parasite control in murine Toxoplasma gondii infection. World J Gastroenterol. 2007 Aug 21;13(31):4207-13., URL: https://pubmed.ncbi.nlm.nih.gov/17696249/,*Zhu DM, Dustin ML, Cairo CW, Thatte HS, Golan DE: Mechanisms of cellular avidity regulation in CD2-CD58-mediated T cell adhesion. ACS Chem Biol. 2006 Nov 21;1(10):649-58., URL: https://pubmed.ncbi.nlm.nih.gov/17168569/,*Dustin ML: Adhesive bond dynamics in contacts between T lymphocytes and glass-supported planar bilayers reconstituted with the immunoglobulin-related adhesion molecule CD58. J Biol Chem. 1997 Jun 20;272(25):15782-8., URL: https://pubmed.ncbi.nlm.nih.gov/9188475/,*Pawlowski NN, Struck D, Grollich K, Kuhl AA, Zeitz M, Liesenfeld O, Hoffmann JC: CD2 deficiency partially prevents small bowel inflammation and improves parasite control in murine Toxoplasma gondii infection. World J Gastroenterol. 2007 Aug 21;13(31):4207-13., URL: https://pubmed.ncbi.nlm.nih.gov/17696249/,

NCBI and Uniprot Product Information

• NCBI GI #: 156071472
• NCBI GeneID: 914
• NCBI Accession #: NP_001758.2
• NCBI GenBank Nucleotide #: NM_001767.3
• UniProt Accession #: P06729; Q96TE5
• Molecular Weight: 39,448 Da
• NCBI Official Full Name: T-cell surface antigen CD2
• NCBI Official Synonym Full Names: CD2 molecule
• NCBI Official Symbol: CD2
• NCBI Official Synonym Symbols: T11; SRBC; LFA-2
• NCBI Protein Information: T-cell surface antigen CD2; CD2 antigen (p50), sheep red blood cell receptor; LFA-3 receptor; T-cell surface antigen T11/Leu-5; erythrocyte receptor; lymphocyte-function antigen-2; rosette receptor
• UniProt Protein Name: T-cell surface antigen CD2
• UniProt Gene Name: CD2
• UniProt Synonym Gene Names: SRBC
• UniProt Entry Name: CD2_HUMAN