IF (Immunofluorescence)
(ImmunofluorescencestainingofJurkatcellswithanti-CD2antibodyVIPVIIIC8(AAA72508). ImmunofluorescenceanalysisofparaformaldehydefixedJurkatcellsonShi-fixcoverslipsstainedwiththechimericrabbitIgGversionofVIPVIIIC8(AAA72508)(1:100dilution)for1hfollowedbyAlexaFluor488secondaryantibody(1:1000dilution),showingmembranestaining.ThenuclearstainisDAPI(blue).Panelsshow,fromleft-right,top-bottom,AAA72508,DAPI,mergedchannelsandanisotypecontrol.TheisotypecontrolwasanunknownspecificityantibodyfollowedbystainingwithAlexaFluor488secondaryantibody.)
CD2 recombinant antibody
Anti-CD2 [VIP VIIIC8], Mouse IgG2a, kappa
Gene Names
CD2; T11; SRBC; LFA-2
Reactivity
Human
Purity
Protein A affinity purified
Synonyms
CD2, Recombinant Antibody; Anti-CD2 [VIP VIIIC8], Mouse IgG2a, kappa; CD2; Anti-CD2 [VIP VIIIC8]; LFA-2; T-cell surface antigen CD2; Erythrocyte receptor; LFA-3 receptor; Rosette receptor; T-cell surface antigen T11/Leu-5; CD2 recombinant antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
Mouse IgG2a kappa
Clone Number
VIP VIIIC8
Specificity
The antibody VIP VIIIC8 recognizes and is directed against CD2.
Purity/Purification
Protein A affinity purified
Form/Format
PBS with 0.02% Proclin 300.
Concentration
1mg/ml (varies by lot)
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.
IF (Immunofluorescence)
(ImmunofluorescencestainingofJurkatcellswithanti-CD2antibodyVIPVIIIC8(AAA72508). ImmunofluorescenceanalysisofparaformaldehydefixedJurkatcellsonShi-fixcoverslipsstainedwiththechimericrabbitIgGversionofVIPVIIIC8(AAA72508)(1:100dilution)for1hfollowedbyAlexaFluor488secondaryantibody(1:1000dilution),showingmembranestaining.ThenuclearstainisDAPI(blue).Panelsshow,fromleft-right,top-bottom,AAA72508,DAPI,mergedchannelsandanisotypecontrol.TheisotypecontrolwasanunknownspecificityantibodyfollowedbystainingwithAlexaFluor488secondaryantibody.)
IF (Immunofluorescence)
(ImmunofluorescencestainingofJurkatcellswithanti-CD2antibodyVIPVIIIC8(AAA72508). ImmunofluorescenceanalysisofparaformaldehydefixedJurkatcellsonShi-fixcoverslipsstainedwiththechimericrabbitIgGversionofVIPVIIIC8(AAA72508)(1:100dilution)for1hfollowedbyAlexaFluor488secondaryantibody(1:1000dilution),showingmembranestaining.ThenuclearstainisDAPI(blue).Panelsshow,fromleft-right,top-bottom,AAA72508,DAPI,mergedchannelsandanisotypecontrol.TheisotypecontrolwasanunknownspecificityantibodyfollowedbystainingwithAlexaFluor488secondaryantibody.)
WB (Western Blot)
(Western Blotusinganti-CD2antibodyVIPVIIIC8(AAA72508). Jurkatcellslysate(35ugproteininRIPAbuffer)wasresolvedonanSDS-PAGEgel,andblotswereprobedwiththechimericrabbitversionofVIPVIIIC8(AAA72508)at0.03ug/mlbeforedetectionusingananti-rabbitsecondaryantibody.Aprimaryincubationof1hwasused,andproteinwasdetectedbychemiluminescence.)
WB (Western Blot)
(Western Blotusinganti-CD2antibodyVIPVIIIC8(AAA72508). Jurkatcellslysate(35ugproteininRIPAbuffer)wasresolvedonanSDS-PAGEgel,andblotswereprobedwiththechimericrabbitversionofVIPVIIIC8(AAA72508)at0.03ug/mlbeforedetectionusingananti-rabbitsecondaryantibody.Aprimaryincubationof1hwasused,andproteinwasdetectedbychemiluminescence.)
FCM/FACS (Flow Cytometry)
(Flowcytometryusingtheanti-CD2antibodyVIPVIIIC8(AAA72508). Jurkatcellswerestainedwiththeanti-unknownspecificityantibodyortherabbitIgGversionofVIPVIIIC8(AAA72508,blueline)atadilutionof1:100for1hatRT.Afterwashing,theboundantibodywasdetectedusingagoatanti-rabbitIgGAlexaFluor488antibodyatadilutionof1:1000andthecellswereanalyzedusingaFACSCantoflow-cytometer.)
FCM/FACS (Flow Cytometry)
(Flowcytometryusingtheanti-CD2antibodyVIPVIIIC8(AAA72508). Jurkatcellswerestainedwiththeanti-unknownspecificityantibodyortherabbitIgGversionofVIPVIIIC8(AAA72508,blueline)atadilutionof1:100for1hatRT.Afterwashing,theboundantibodywasdetectedusingagoatanti-rabbitIgGAlexaFluor488antibodyatadilutionof1:1000andthecellswereanalyzedusingaFACSCantoflow-cytometer.)
Related Product Information for CD2 recombinant antibody
This antibody was raised by immunizing mice against CD2.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
39,448 Da
NCBI Official Full Name
T-cell surface antigen CD2
NCBI Official Synonym Full Names
CD2 molecule
NCBI Official Symbol
CD2
NCBI Official Synonym Symbols
T11; SRBC; LFA-2
NCBI Protein Information
T-cell surface antigen CD2; CD2 antigen (p50), sheep red blood cell receptor; LFA-3 receptor; T-cell surface antigen T11/Leu-5; erythrocyte receptor; lymphocyte-function antigen-2; rosette receptor
UniProt Protein Name
T-cell surface antigen CD2
UniProt Gene Name
CD2
UniProt Synonym Gene Names
SRBC
UniProt Entry Name
CD2_HUMAN
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Product Notes
The CD2 cd2 (Catalog #AAA72508) is a Recombinant Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-CD2 [VIP VIIIC8], Mouse IgG2a, kappa reacts with Human and may cross-react with other species as described in the data sheet. It is sometimes possible for the material contained within the vial of "CD2, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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