Rabbit LGR5 (GPR49) Polyclonal Antibody | anti-LGR5 antibody

LGR5 (GPR49) Antibody (Center)

Cat. Num. AAA285606

• Gene Names: LGR5; FEX; HG38; GPR49; GPR67; GRP49
• Reactivity: Human (Predicted Reactivity: Bovine)
• Applications: Immunohistochemistry, Western Blot, ELISA, Flow Cytometry, Functional Assay
• Purity: Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: LGR5 (GPR49), Antibody; LGR5 (GPR49) Antibody (Center); Leucine-rich repeat-containing G-protein coupled receptor 5; G-protein coupled receptor 49; G-protein coupled receptor 67; G-protein coupled receptor HG38; LGR5; GPR49; GPR67; anti-LGR5 antibody

• Host: Rabbit
• Reactivity: Human (Predicted Reactivity: Bovine)
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: This LGR5 (GPR49) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 443-473 amino acids from the Central region of human LGR5 (GPR49).
• Purity/Purification: Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Sequence Positions: 443-473
• Sequence Length: 883

• Applicable Applications for anti-LGR5 antibody: IHC (Immunohistochemistry), WB (Western Blot), ELISA, FCM/FACS (Flow Cytometry)
• Function: Receptor for R-spondins that potentiates the canonical Wnt signaling pathway and acts as a stem cell marker of the intestinal epithelium and the hair follicle. Upon binding to R- spondins (RSPO1, RSPO2, RSPO3 or RSPO4), associates with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. In contrast to classical G-protein coupled receptors, does not activate heterotrimeric G-proteins to transduce the signal. Involved in the development and/or maintenance of the adult intestinal stem cells during postembryonic development.
• Cellular location: Cell membrane; Multi-pass membrane protein. Golgi apparatus, trans-Golgi network membrane; Multi-pass membrane protein. Note=Rapidly and constitutively internalized to the trans-Golgi network at steady state. Internalization to the trans- Golgi network may be the result of phosphorylation at Ser-861 and Ser-864; however, the phosphorylation event has not been proven.
• Tissue location: Expressed in skeletal muscle, placenta, spinal cord, and various region of brain. Expressed at the base of crypts in colonic and small mucosa stem cells. In premalignant cancer expression is not restricted to the cript base. Overexpressed in cancers of the ovary, colon and liver
• Preparation and Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

FCM/FACS (Flow Cytometry)

(LGR5 (GPR49) Antibody (Center) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

WB (Western Blot)

(LGR5/GPR49 Antibody (Center)(AAA285606) western blot analysis in HepG2 cell line lysates (35ug/lane). This demonstrates the LGR5/GPR49 antibody detected the LGR5/GPR49 protein (arrow)(Kindly offered by Dr. Li).)

WB (Western Blot)

(Western blot analysis of anti-LGR5/GPR49Antibody (Center) (AAA285606) in K562cell line lysates (35ug/lane). LGR5/GPR49(arrow) was detected using the purified Pab.)

IHC (Immunohiostchemistry)

(Immunohistochemical analysis of paraffin-embedded Human brain section using Pink1(AAA285606). AAA285606 was diluted at 1:100 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DABstaining.)

IHC (Immunohistochemistry)

(Formalin-fixed and paraffin-embedded human brain tissue reacted with LGR5/GPR49 antibody (Center) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Related Product Information for anti-LGR5 antibody

LGR5/GPR49 is an orphan receptor. It may be an important receptor for signals controlling growth and differentiation of specific embryonic tissues.

Product Categories/Family for anti-LGR5 antibody

Cancer; Signal Transduction; GPCR Antibodies

References

Barker,N., Nature 449 (7165), 1003-1007 (2007) McClanahan,T., Cancer Biol. Ther. 5 (4), 419-426 (2006) Yamamoto,Y., Hepatology 37 (3), 528-533 (2003) Hsu,S.Y., Mol. Endocrinol. 14 (8), 1257-1271 (2000)

NCBI and Uniprot Product Information

• NCBI GI #: 469608414
• NCBI GeneID: 8549
• NCBI Accession #: NP_001264155.1; NP_003658.1
• NCBI GenBank Nucleotide #: NM_001277226.1
• UniProt Accession #: O75473; Q4VAM0; Q4VAM2; Q9UP75; D8MCT0
• NCBI Official Full Name: leucine-rich repeat-containing G-protein coupled receptor 5 isoform 2
• NCBI Official Synonym Full Names: leucine-rich repeat containing G protein-coupled receptor 5
• NCBI Official Symbol: LGR5
• NCBI Official Synonym Symbols: FEX; HG38; GPR49; GPR67; GRP49
• NCBI Protein Information: leucine-rich repeat-containing G-protein coupled receptor 5
• UniProt Protein Name: Leucine-rich repeat-containing G-protein coupled receptor 5
• UniProt Gene Name: LGR5
• UniProt Synonym Gene Names: GPR49; GPR67
• UniProt Entry Name: LGR5_HUMAN