IF (Immunofluorescence)
(LGR5/GPR49 Antibody (loop2) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the LGR5/GPR49 antibody detected the LGR5/GPR49 protein (arrow).)
Rabbit LGR5/GPR49 Polyclonal Antibody | anti-LGR5 antibody
LGR5/GPR49 Antibody (loop2)
IF (Immunofluorescence)
(LGR5/GPR49 Antibody (loop2) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the LGR5/GPR49 antibody detected the LGR5/GPR49 protein (arrow).)
IF (Immunofluorescence)
(LGR5/GPR49 Antibody (loop2) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the LGR5/GPR49 antibody detected the LGR5/GPR49 protein (arrow).)
IF (Immunofluorescence)
(Fluorescent confocal image of HeLa cells stained with LGR5/GPR49 (loop2) antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AAA28712 LGR5 (loop2) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). Note the highly specific localization of the LGR5 immunosignal to the nucleus and cytoplasm, supported by Human Protein Atlas Data (http://www.proteinatlas.org/ENSG00000139292).)
IF (Immunofluorescence)
(Fluorescent confocal image of HeLa cells stained with LGR5/GPR49 (loop2) antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AAA28712 LGR5 (loop2) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). Note the highly specific localization of the LGR5 immunosignal to the nucleus and cytoplasm, supported by Human Protein Atlas Data (http://www.proteinatlas.org/ENSG00000139292).)
WB (Western Blot)
(Western blot analysis of lysates from Hela, HepG2, SH-SY5Y cell line (from left to right), using LGR5/GPR49 Antibody (loop2). AAA28712 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.)
WB (Western Blot)
(Western blot analysis of lysates from Hela, HepG2, SH-SY5Y cell line (from left to right), using LGR5/GPR49 Antibody (loop2). AAA28712 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.)
WB (Western Blot)
(All lanes : Anti-LGR5 Antibody (loop2) at 1:2000 dilutionLane 1: Hela whole cell lysatesLane 2: HepG2whole cell lysatesLane 3: mouse cerebellum lysatesLane 4: mouse skeletal muscle lysatesLane 5: SH-SY5Y whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 100 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(All lanes : Anti-LGR5 Antibody (loop2) at 1:2000 dilutionLane 1: Hela whole cell lysatesLane 2: HepG2whole cell lysatesLane 3: mouse cerebellum lysatesLane 4: mouse skeletal muscle lysatesLane 5: SH-SY5Y whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 100 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(All lanes : Anti-LGR5 Antibody (loop2) at 1:2000 dilutionLane 1: Hela whole cell lysatesLane 2: HepG2 whole cell lysatesLane 3: mouse skeletal muscle lysatesLane 4: SH-SY5Y whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 100 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(All lanes : Anti-LGR5 Antibody (loop2) at 1:2000 dilutionLane 1: Hela whole cell lysatesLane 2: HepG2 whole cell lysatesLane 3: mouse skeletal muscle lysatesLane 4: SH-SY5Y whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 100 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(All lanes : Anti-LGR5 Antibody (loop2) at 1:2000 dilutionLane 1: mouse skeletal muscle lysatesLane 2: rat skeletal muscle lysatesLane 3: Hela whole cell lysatesLane 4: HepG2 whole cell lysatesLane 5: SH-SY5Y whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 100 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(All lanes : Anti-LGR5 Antibody (loop2) at 1:2000 dilutionLane 1: mouse skeletal muscle lysatesLane 2: rat skeletal muscle lysatesLane 3: Hela whole cell lysatesLane 4: HepG2 whole cell lysatesLane 5: SH-SY5Y whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 100 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(All lanes : Anti-LGR5 Antibody (loop2) at 1:2000 dilutionLane 1: HepG2 whole cell lysatesLane 2: SH-SY5Y whole cell lysatesLane 3: R.skeletal muscle lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 100 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(All lanes : Anti-LGR5 Antibody (loop2) at 1:2000 dilutionLane 1: HepG2 whole cell lysatesLane 2: SH-SY5Y whole cell lysatesLane 3: R.skeletal muscle lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 100 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
of brain. Expressed at the base of crypts in colonic and small mucosa
stem cells. In premalignant cancer expression is not restricted to the
cript base. Overexpressed in cancers of the ovary, colon and liver.
1. Barker,N., Nature 449 (7165),1003-1007 (2007)
2. Hsu,S.Y., Mol. Endocrinol. 14 (8), 1257-1271 (2000).
3. Yamamoto Y., Sakamoto M., Fujii G., Tsuiji H., Kenetaka K., Asaka M., Hirohashi S. Overexpression of orphan G-protein-coupled receptor, Gpr49, in human hepatocellular carcinomas with beta-catenin mutations. Hepatology 37:528-533(2003).
4. McClanahan T., Koseoglu S., Smith K., Grein J., Gustafson E., Black S., Kirschmeier P., Samatar A.A. Identification of overexpression of orphan G protein-coupled receptor GPR49 in human colon and ovarian primary tumors. Cancer Biol. Ther. 5:419-426(2006).
6. Becker L., Huang Q., Mashimo H. Immunostaining of Lgr5, an intestinal stem cell marker, in normal and premalignant human gastrointestinal tissue. ScientificWorldJournal 8:1168-1176(2008)
References for HeLa cell line:
1. Scherer WF, Syverton JT, Gey GO (May 1953). "Studies on the propagation in vitro of poliomyelitis viruses. IV. Viral multiplication in a stable strain of human malignant epithelial cells (strain HeLa) derived from an epidermoid carcinoma of the cervix". J. Exp. Med. 97 (5): 695-710. [PubMed:13052828].
2. Macville M, Schröck E, Padilla-Nash H, Keck C, Ghadimi BM, Zimonjic D, Popescu N, Ried T (January 1999). "Comprehensive and definitive molecular cytogenetic characterization of HeLa cells by spectral karyotyping". Cancer Res. 59 (1): 141-50. [PubMed: 9892199].
3. Rahbari R, Sheahan T, Modes V, Collier P, Macfarlane C, Badge RM (April 2009). "A novel L1 retrotransposon marker for HeLa cell line identification". BioTechniques 46 (4): 277-84. [PubMed: 19450234].
4. Capes-Davis A, Theodosopoulos G, Atkin I, Drexler HG, Kohara A, MacLeod RA, Masters JR, Nakamura Y, Reid YA, Reddel RR, Freshney RI (July 2010). "Check your cultures! A list of cross-contaminated or misidentified cell lines". Int. J. Cancer 127 (1): 1-8. [PubMed:20143388].
NCBI and Uniprot Product Information
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Product Notes
The LGR5 lgr5 (Catalog #AAA28712) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 689-719. The LGR5/GPR49 Antibody (loop2) reacts with Human, mouse, rat and may cross-react with other species as described in the data sheet. AAA Biotech's LGR5/GPR49 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunofluorescence (IF), Flow Cytometry (FC/FACS). WB~~1:1000. Researchers should empirically determine the suitability of the LGR5 lgr5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LGR5/GPR49, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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