WB (Western Blot)
(Detection of human and mouse Liprin beta 1 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Liprin beta 1 antibody AAA212808 (lot AAA212808-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
Rabbit anti-Human, Mouse Liprin beta 1 Polyclonal Antibody | anti-PPFIBP1 antibody
Rabbit anti-Liprin beta 1 Antibody, Affinity Purified
Reactivity
Human, Mouse
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
Liprin beta 1, Antibody; Rabbit anti-Liprin beta 1 Antibody, Affinity Purified; hSGT2; hSgt2p; L2; liprin related protein; liprin-beta-1; Protein tyrosine phosphatase receptor type f polypeptide-interacting protein-binding protein 1; protein-tyrosine phosphatase receptor-type f polypeptide-interacting protein-binding protein 1; PTPRF interacting protein, binding protein 1 (liprin beta 1); PTPRF-interacting protein-binding protein 1; SGT2; Liprin-beta-1; PPFIA binding protein 1; anti-PPFIBP1 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-PPFIBP1 antibody
IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 175 and 225
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human and mouse Liprin beta 1 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Liprin beta 1 antibody AAA212808 (lot AAA212808-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
WB (Western Blot)
(Detection of human and mouse Liprin beta 1 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Liprin beta 1 antibody AAA212808 (lot AAA212808-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IP (Immunoprecipitation)
(Detection of human Liprin beta 1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Liprin beta 1 antibody AAA212808 (lot AAA212808-1) used for IP at 6 ug per reaction. Liprin beta 1 was also immunoprecipitated by rabbit anti-Liprin beta 1 antibody BL17605. For blotting immunoprecipitated Liprin beta 1, AAA212808 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IP (Immunoprecipitation)
(Detection of human Liprin beta 1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Liprin beta 1 antibody AAA212808 (lot AAA212808-1) used for IP at 6 ug per reaction. Liprin beta 1 was also immunoprecipitated by rabbit anti-Liprin beta 1 antibody BL17605. For blotting immunoprecipitated Liprin beta 1, AAA212808 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
Related Product Information for anti-PPFIBP1 antibody
Liprin beta 1 is a member of the LAR protein-tyrosine phosphatase-interacting protein (liprin) family. Liprins interact with members of LAR family of transmembrane protein tyrosine phosphatases, which are known to be important for axon guidance and mammary gland development. It has been proposed that liprins are multivalent proteins that form complex structures and act as scaffolds for the recruitment and anchoring of LAR family of tyrosine phosphatases. Liprin beta 1 was found to interact with S100A4, a calcium-binding protein related to tumor invasiveness and metastasis. In vitro experiments demonstrated that the interaction inhibited the phosphorylation of this protein by protein kinase C and protein kinase CK2 [taken from NCBI Entrez Gene (Gene ID: 8496)].
Product Categories/Family for anti-PPFIBP1 antibody
NCBI and Uniprot Product Information
Similar Products
Product Notes
The PPFIBP1 ppfibp1 (Catalog #AAA212808) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-Liprin beta 1 Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Liprin beta 1 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the PPFIBP1 ppfibp1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Liprin beta 1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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