Rabbit LYVE1 Polyclonal Antibody | anti-LYVE1 antibody

LYVE1 Antibody

Cat. Num. AAA329400

• Gene Names: LYVE1; HAR; XLKD1; LYVE-1; CRSBP-1
• Reactivity: Human, Mouse, Rat, Monkey
• Applications: ELISA, Immunohistochemistry, Western Blot
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
• Synonyms: LYVE1, Antibody; LYVE1 Antibody; Cell surface retention sequence-binding protein 1; CRSBP 1; CRSBP-1; CRSBP1; extracellular link domain containing 1; extracellular link domain-containing 1; Extracellular link domain-containing protein 1; HAR; Hyaluronic acid receptor; Lymphatic endothelium specific hyaluronan receptor; lymphatic vessel endothelial hyaluronan receptor 1; Lymphatic vessel endothelial hyaluronic acid receptor 1; LYVE 1; LYVE-1; LYVE1; LYVE1_HUMAN; XLKD1; anti-LYVE1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat, Monkey
• Clonality: Polyclonal
• Isotype: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH7.4, 150mM NaCl and glycerol.
• Specificity: LYVE1 Antibody detects endogenous levels of total LYVE1.; Tissue Specificity: Mainly expressed in endothelial cells lining lymphatic vessels.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
• Form/Format: Liquid. Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH7.4, 150mM NaCl and glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-LYVE1 antibody: ELISA (Peptide ELISA), IHC (Immunohistochemistry), WB (Western Blot)
• Immunogen: A synthesized peptide derived from human LYVE1, corresponding to a region within the internal amino acids.
• Conjugation: Unconjugated
• Fragment: Fab fragment
• Post Translational Modifications: O-glycosylated.
• Subunit Structure: Homodimer; disulfide-linked. Interacts with PDGFB and IGFBP3. Forms a transient ternary complex with PDGFB and PDGFRB in TGN (By similarity).
• Subcellular Location: Membrane>Single-pass type I membrane protein.; Note: Localized to the plasma membrane and in vesicles near extranuclear membranes which may represent trans-Golgi network (TGN) and endosomes/prelysosomeal compartments. Undergoes ligand-dependent internalization and recycling at the cell surface.
• Preparation and Storage: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH7.4, 150mM NaCl and glycerol.

IHC (Immunohistochemistry)

(At 1/100 staining Human pancreatic cancer and adjacent nomal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry)

(At 1/100 staining Mouse liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemisry)

(At 1/100 staining Mouse pancreatic tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

WB (Western Blot)

(Western blot analysis of LYVE1 Antibody expression in MCF cells lysates.The lane on the left was treated with the antigen-specific peptide.)

WB (Western Blot)

(Western blot analysis of extracts from VERO cells, using LYVE1 Antibody. The lane on the left was treated with blocking peptide.)

Related Product Information for anti-LYVE1 antibody

Ligand-specific transporter trafficking between intracellular organelles (TGN) and the plasma membrane. Plays a role in autocrine regulation of cell growth mediated by growth regulators containing cell surface retention sequence binding (CRS). May act as a hyaluronan (HA) transporter, either mediating its uptake for catabolism within lymphatic endothelial cells themselves, or its transport into the lumen of afferent lymphatic vessels for subsequent re-uptake and degradation in lympHnodes.
Ligand-specific transporter trafficking between intracellular organelles (TGN) and the plasma membrane. Plays a role in autocrine regulation of cell growth mediated by growth regulators containing cell surface retention sequence binding (CRS). May act as an hyaluronan (HA) transporter, either mediating its uptake for catabolism within lymphatic endothelial cells themselves, or its transport into the lumen of afferent lymphatic vessels for subsequent re-uptake and degradation in lympHnodes.

NCBI and Uniprot Product Information

• NCBI GI #: 10800122
• NCBI GeneID: 10894
• UniProt Accession #: Q9Y5Y7; Q8TC18; Q9UNF4
• Molecular Weight: 35,213 Da
• NCBI Official Full Name: hyaluronic acid receptor
• NCBI Official Synonym Full Names: lymphatic vessel endothelial hyaluronan receptor 1
• NCBI Official Symbol: LYVE1
• NCBI Official Synonym Symbols: HAR; XLKD1; LYVE-1; CRSBP-1
• NCBI Protein Information: lymphatic vessel endothelial hyaluronic acid receptor 1; extracellular link domain containing 1; extracellular link domain-containing 1; extracellular link domain-containing protein 1; cell surface retention sequence binding protein-1; cell surface retent
• UniProt Protein Name: Lymphatic vessel endothelial hyaluronic acid receptor 1
• UniProt Gene Name: LYVE1
• UniProt Synonym Gene Names: CRSBP1; HAR; XLKD1; LYVE-1; CRSBP-1
• UniProt Entry Name: LYVE1_HUMAN