WB (Western Blot)
(Detection of human MAD1 by western blot and immunoprecipitation. Samples: Whole cell lysate (50 ug/lane in A; 50 ug input in B, 1 mg for IP in B) from HeLa cells. In A, HeLa cells were treated with Nocodazole (+) or mock treated (-). Antibody: Affinity purified rabbit anti-MAD1 antibody AAA210892 used at 0.1 ug/ml for western blot (A) and at 1 ug/mg lysate for IP (B). MAD1 was also immunoprecipitated using rabbit anti-MAD1 antibodies BL1689 and BL1690 using 1 ug/mg lysate. In B, MAD1 in input and immunoprecipitate was detected using BL1689 at 0.2 ug/ml. Detection: Chemiluminescence with exposure times of 10 minutes (A) and 5 minutes (B).)
Rabbit anti-Human MAD1 Polyclonal Antibody | anti-MAD1 antibody
Rabbit anti-MAD1 Antibody, Affinity Purified
Gene Names
MAD1L1; MAD1; PIG9; TP53I9; TXBP181
Reactivity
Human
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
MAD1, Antibody; Rabbit anti-MAD1 Antibody, Affinity Purified; MAD1; mitotic-arrest deficient 1, yeast, homolog-like 1; TXBP181; tumor protein p53 inducible protein 9; TP53I9; tax-binding protein 181; PIG9; mitotic spindle assembly checkpoint protein MAD1; mitotic checkpoint MAD1 protein homolog; mitotic arrest deficient 1-like protein 1; MAD1 mitotic arrest deficient like 1; HsMAD1; hMAD1; MAD1-like protein 1; Mitotic spindle assembly checkpoint protein MAD1; mitotic arrest deficient 1 like 1; anti-MAD1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-MAD1 antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 1 and 50
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human MAD1 by western blot and immunoprecipitation. Samples: Whole cell lysate (50 ug/lane in A; 50 ug input in B, 1 mg for IP in B) from HeLa cells. In A, HeLa cells were treated with Nocodazole (+) or mock treated (-). Antibody: Affinity purified rabbit anti-MAD1 antibody AAA210892 used at 0.1 ug/ml for western blot (A) and at 1 ug/mg lysate for IP (B). MAD1 was also immunoprecipitated using rabbit anti-MAD1 antibodies BL1689 and BL1690 using 1 ug/mg lysate. In B, MAD1 in input and immunoprecipitate was detected using BL1689 at 0.2 ug/ml. Detection: Chemiluminescence with exposure times of 10 minutes (A) and 5 minutes (B).)
WB (Western Blot)
(Detection of human MAD1 by western blot and immunoprecipitation. Samples: Whole cell lysate (50 ug/lane in A; 50 ug input in B, 1 mg for IP in B) from HeLa cells. In A, HeLa cells were treated with Nocodazole (+) or mock treated (-). Antibody: Affinity purified rabbit anti-MAD1 antibody AAA210892 used at 0.1 ug/ml for western blot (A) and at 1 ug/mg lysate for IP (B). MAD1 was also immunoprecipitated using rabbit anti-MAD1 antibodies BL1689 and BL1690 using 1 ug/mg lysate. In B, MAD1 in input and immunoprecipitate was detected using BL1689 at 0.2 ug/ml. Detection: Chemiluminescence with exposure times of 10 minutes (A) and 5 minutes (B).)
WB (Western Blot)
(Detection of human MAD1 by western blot. Samples: Whole cell lysate (50 ug) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MAD1 antibody AAA210892 (lot AAA210892-1) used for WB at 0.06 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
WB (Western Blot)
(Detection of human MAD1 by western blot. Samples: Whole cell lysate (50 ug) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MAD1 antibody AAA210892 (lot AAA210892-1) used for WB at 0.06 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IHC (Immunohistochemistry)
(Detection of human MAD1 by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-MAD1 (Cat. No. AAA210892 Lot1) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human MAD1 by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-MAD1 (Cat. No. AAA210892 Lot1) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
Related Product Information for anti-MAD1 antibody
MAD1 (Mitotic Arrest Deficiency 1) is a component of the mitotic spindle-assembly checkpoint that ensures that all chromosomes are aligned at the metaphase plate before the onset of anaphase. MAD1 functions as a homodimer that interacts with and regulates MAD2. MAD1 may play a role in tumor suppression.
Product Categories/Family for anti-MAD1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
72,286 Da
NCBI Official Full Name
mitotic spindle assembly checkpoint protein MAD1 isoform a
NCBI Official Synonym Full Names
mitotic arrest deficient 1 like 1
NCBI Official Symbol
MAD1L1
NCBI Official Synonym Symbols
MAD1; PIG9; TP53I9; TXBP181
NCBI Protein Information
mitotic spindle assembly checkpoint protein MAD1
UniProt Protein Name
Mitotic spindle assembly checkpoint protein MAD1
UniProt Gene Name
MAD1L1
UniProt Synonym Gene Names
MAD1; TXBP181; MAD1-like protein 1; HsMAD1; hMAD1
UniProt Entry Name
MD1L1_HUMAN
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Product Notes
The MAD1 mad1l1 (Catalog #AAA210892) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-MAD1 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MAD1 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the MAD1 mad1l1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MAD1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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