WB (Western Blot)
(Detection of human MAML1 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, A-549, MCF-7, and GaMG cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MAML1 antibody AAA211082 (lot AAA211082-2) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Rabbit anti-Human MAML1 Polyclonal Antibody | anti-MAML1 antibody
Rabbit anti-MAML1 Antibody, Affinity Purified
Gene Names
MAML1; Mam1; Mam-1
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
MAML1, Antibody; Rabbit anti-MAML1 Antibody, Affinity Purified; Mam1; Mam-1; mastermind homolog; mastermind-like 1; mastermind-like protein 1; Mastermind-like protein 1; mastermind like transcriptional coactivator 1; anti-MAML1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-MAML1 antibody
IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 75 and 125
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human MAML1 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, A-549, MCF-7, and GaMG cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MAML1 antibody AAA211082 (lot AAA211082-2) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
WB (Western Blot)
(Detection of human MAML1 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, A-549, MCF-7, and GaMG cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MAML1 antibody AAA211082 (lot AAA211082-2) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human MAML1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-MAML1 antibody AAA211082 (lot 2) used for IP at 6 ug per reaction. MAML1 was also immunoprecipitated by a previous lot of this antibody (lot 1) and rabbit anti-MAML1 antibody A300-673A. For blotting immunoprecipitated MAML1, AAA211082 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IP (Immunoprecipitation)
(Detection of human MAML1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-MAML1 antibody AAA211082 (lot 2) used for IP at 6 ug per reaction. MAML1 was also immunoprecipitated by a previous lot of this antibody (lot 1) and rabbit anti-MAML1 antibody A300-673A. For blotting immunoprecipitated MAML1, AAA211082 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
Related Product Information for anti-MAML1 antibody
There are 3 MAML (mastermind-like) proteins in mammals (MAML1-3). MAML1 is a critical coactivator for NOTCH signaling. MAML1 associates with NOTCH and CSL in a ternary complex and functions to activate transcription of HES and hairy-related basic helix-loop-helix transcription factors. MAML1 has been observed to interact with p300 to acetylate histones H3 and H4. MAML1 also interacts with p53 and functions independent of NOTCH signaling, as a coactivator of p53 target genes.
Product Categories/Family for anti-MAML1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
108,054 Da
NCBI Official Full Name
mastermind-like protein 1
NCBI Official Synonym Full Names
mastermind-like 1 (Drosophila)
NCBI Official Symbol
MAML1
NCBI Official Synonym Symbols
Mam1; Mam-1
NCBI Protein Information
mastermind-like protein 1; MAML1; mastermind homolog
UniProt Protein Name
Mastermind-like protein 1
UniProt Gene Name
MAML1
UniProt Synonym Gene Names
Mam-1
UniProt Entry Name
MAML1_HUMAN
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Product Notes
The MAML1 maml1 (Catalog #AAA211082) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-MAML1 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MAML1 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the MAML1 maml1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MAML1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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