Rabbit anti-Human MBD4 Polyclonal Antibody | anti-MBD4 antibody

Anti-MBD4 Antibody, Rabbit Polyclonal

Cat. Num. AAA254332

• Gene Names: MBD4; MED1
• Reactivity: Human
• Applications: Immunoprecipitation, Western Blot
• Purity: Protein A & Antigen Affinity
• Synonyms: MBD4, Antibody; Anti-MBD4 Antibody, Rabbit Polyclonal; MBD4 Antibody, Rabbit PAb, Antigen Affinity Purified; methyl-CpG binding domain 4 DNA glycosylase; Anti-MED1 Antibody; anti-MBD4 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human MBD4
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-MBD4 antibody: IP (Immunoprecipitation), WB (Western Blot)
• Immunogen: A synthetic peptide corresponding to the center region of the Human MBD4
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human MBD4, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

WB (Western Blot)

(Anti-MBD4 rabbit polyclonal antibody at 1:500 dilutionLane A: Hela Whole Cell LysateLane B: MCF7 Whole Cell LysateLane C: K562 Whole Cell LysateLane D: A549 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:66 kDaObserved band size:53 kDa(We are unsure as to the identity of these extra bands.))

IP (Immunoprecipitation)

(MBD4 was immunoprecipitated using:Lane A:0.5 mg Hela Whole Cell LysateLane B:0.5 mg MCF-7 Whole Cell Lysate0.5 uL anti-MBD4 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein G.Primary antibody:Anti-MBD4 rabbit polyclonal antibody,at 1:500 dilutionSecondary antibody:Clean-Blot IP Detection Reagent (HRP) at 1:500 dilutionDeveloped using the DAB staining technique.Performed under reducing conditions.Predicted band size: 66 kDaObserved band size: 53 kDa)

Related Product Information for anti-MBD4 antibody

MBD4 is the only methyl-CpG binding protein that possesses a C-terminal glycosylase domain. MBD4 contains a methyl-CpG-binding domain which provides the localization of the enzyme at the CpG sites, and a DNA glycosylase domain that is responsible for the catalytic activity. Methyl-CpG binding domain protein 4 (MBD4) is a DNA glycosylase that can remove 5-fluorodeoxyuracil from DNA as well as repair T:G or U:G mismatches. MBD4 is a target for frameshift mutation with DNA mismatch repair (MMR) deficiency, creating a truncated MBD4 protein (TruMBD4) that lacks its glycosylase domain. MBD4 (methyl-CpG binding domain protein 4) is an important G: T glycosylase that can identify T-G mismatches. It plays a role in active demethylation through base excision repair. Overexpression of MBD4 gene can cause the demethylation of numerous genes, and the remethylation of MBD4-associated genes can occur when the MBD4 gene is knocked out.

NCBI and Uniprot Product Information

• NCBI GI #: 50400819
• NCBI GeneID: 8930
• UniProt Accession #: O95243; Q2MD36; Q7Z4T3; Q96F09; B4DZN2; D3DNC3; D3DNC4; E9PEE4
• Molecular Weight: 580
• NCBI Official Full Name: Methyl-CpG-binding domain protein 4
• NCBI Official Synonym Full Names: methyl-CpG binding domain protein 4
• NCBI Official Symbol: MBD4
• NCBI Official Synonym Symbols: MED1
• NCBI Protein Information: methyl-CpG-binding domain protein 4; G/T mismatch glycosylase; G/U mismatch glycosylase; methyl-CpG-binding protein MBD4; 3,N(4)-ethenocytosine glycosylase; methyl-CpG-binding endonuclease 1; mismatch-specific DNA N-glycosylase; putative methyl-CpG bindin
• UniProt Protein Name: Methyl-CpG-binding domain protein 4
• UniProt Gene Name: MBD4
• UniProt Synonym Gene Names: MED1
• UniProt Entry Name: MBD4_HUMAN