IHC (Immunohistochemisry)
(MED1/TRAP220 Antibody-Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE))
Rabbit MED1/TRAP220 Polyclonal Antibody | anti-MED1 antibody
MED1/TRAP220 Rabbit anti-Human Polyclonal (Internal) Antibody
Gene Names
MED1; PBP; CRSP1; RB18A; TRIP2; PPARBP; CRSP200; DRIP205; DRIP230; PPARGBP; TRAP220
Reactivity
Mouse, Rat, Human, Monkey
Applications
Western Blot, Immunohistochemistry, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
Immunoaffinity purified
Synonyms
MED1/TRAP220, Antibody; MED1/TRAP220 Rabbit anti-Human Polyclonal (Internal) Antibody; IHC-plus MED1/TRAP220 Antibody (Internal); MED1; ARC205; CRSP1; DRIP205; DRIP230; Mediator complex subunit 1; PBP; PPARG binding protein; RB18A; TRIP-2; TRIP2; PPARBP; CRSP200; p53 regulatory protein RB18A; PPAR binding protein; PPAR-binding protein; PPARGBP; TR-interacting protein 2; TRAP220; anti-MED1 antibody
Host
Rabbit
Reactivity
Mouse, Rat, Human, Monkey
Clonality
Polyclonal
Specificity
Recognizes endogenous levels of TRAP220 protein.
Purity/Purification
Immunoaffinity purified
Form/Format
PBS, pH7.3, 0.01% Sodium Azide, 30% Glycerol
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-MED1 antibody
WB (Western Blot), IHC (Immunohistochemistry), IHC (Immunohistochemistry), IF (Immunofluorescence), ICC (Immunocytochemistry)
Target
Human MED1/TRAP220
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the central region of human TRAP220.
Conjugation
Unconjugated
Epitope
Internal
Preparation and Storage
Store at -20 degree C. Aliquot to avoid freeze/thaw cycles.
IHC (Immunohistochemisry)
(MED1/TRAP220 Antibody-Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE))
IHC (Immunohistochemisry)
(MED1/TRAP220 Antibody-Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE))
WB (Western Blot)
(MED1/TRAP220 Antibody-Western blot analysis of TRAP220 expression in HeLa (A); HUVEC (B); Jurkat (C) whole cell lysates.)
WB (Western Blot)
(MED1/TRAP220 Antibody-Western blot analysis of TRAP220 expression in HeLa (A); HUVEC (B); Jurkat (C) whole cell lysates.)
ICC (Immunocytochemistry)
(MED1/TRAP220 Antibody-Immunofluorescent analysis of TRAP220 staining in HUVEC cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)
ICC (Immunocytochemistry)
(MED1/TRAP220 Antibody-Immunofluorescent analysis of TRAP220 staining in HUVEC cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)
Related Product Information for anti-MED1 antibody
TRAP220 antibody is an unconjugated rabbit polyclonal antibody to TRAP220 (MED1)(Internal) from human. It is reactive with human, mouse, rat and other species. Validated for ICC, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
168,478 Da
NCBI Official Full Name
mediator of RNA polymerase II transcription subunit 1
NCBI Official Synonym Full Names
mediator complex subunit 1
NCBI Official Symbol
MED1
NCBI Official Synonym Symbols
PBP; CRSP1; RB18A; TRIP2; PPARBP; CRSP200; DRIP205; DRIP230; PPARGBP; TRAP220
NCBI Protein Information
mediator of RNA polymerase II transcription subunit 1; ARC205; TRIP-2; PPAR binding protein; PPAR-binding protein; PPARG binding protein; TR-interacting protein 2; p53 regulatory protein RB18A; thyroid receptor interacting protein 2; thyroid receptor-inte
UniProt Protein Name
Mediator of RNA polymerase II transcription subunit 1
UniProt Gene Name
MED1
UniProt Synonym Gene Names
ARC205; CRSP1; CRSP200; DRIP205; DRIP230; PBP; PPARBP; PPARGBP; RB18A; TRAP220; TRIP2; ARC205; PBP; PPAR-binding protein; Trap220; TR-interacting protein 2; TRIP-2
UniProt Entry Name
MED1_HUMAN
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Product Notes
The MED1 med1 (Catalog #AAA162745) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MED1/TRAP220 Rabbit anti-Human Polyclonal (Internal) Antibody reacts with Mouse, Rat, Human, Monkey and may cross-react with other species as described in the data sheet. AAA Biotech's MED1/TRAP220 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IHC (Immunohistochemistry), IHC (Immunohistochemistry), IF (Immunofluorescence), ICC (Immunocytochemistry). Researchers should empirically determine the suitability of the MED1 med1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MED1/TRAP220, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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