IP (Immunoprecipitation)
(Immunoprecipitating MFN1 in Jurkat whole cell lysateLane 1: Rabbit monoclonal IgG(1ug)instead of AAA231837 in Jurkat whole cell lysate. For western blotting, a HRP-conjugated light chain specific antibody was used as the Secondary antibody (1/50000)Lane 2: AAA231837(4ug)+ Jurkat whole cell lysate(500ug)Lane 3: Jurkat whole cell lysate (20ug))
Rabbit anti-Human MFN1 Polyclonal Antibody | anti-MFN1 antibody
MFN1 Antibody
Gene Names
MFN1; hfzo1; hfzo2
Reactivity
Human
Applications
Immunoprecipitation, Immunohistochemistry, Western Blot, ELISA
Purity
Antigen Affinity Purified
Synonyms
MFN1, Antibody; MFN1 Antibody; Mitofusin-1; Fzo homolog; Transmembrane GTPase MFN1; MFN1; anti-MFN1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid
Sequence Length
741
Applicable Applications for anti-MFN1 antibody
IP (Immunoprecipitation), IHC (Immunohistochemistry), WB (Western Blot), ELISA
Species
Human
Immunogen
Recombinant human Mitofusin-1 protein (C-120AA)
Conjugate
Non-conjugated
Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-30363 / sc-50330
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.
IP (Immunoprecipitation)
(Immunoprecipitating MFN1 in Jurkat whole cell lysateLane 1: Rabbit monoclonal IgG(1ug)instead of AAA231837 in Jurkat whole cell lysate. For western blotting, a HRP-conjugated light chain specific antibody was used as the Secondary antibody (1/50000)Lane 2: AAA231837(4ug)+ Jurkat whole cell lysate(500ug)Lane 3: Jurkat whole cell lysate (20ug))
IP (Immunoprecipitation)
(Immunoprecipitating MFN1 in Jurkat whole cell lysateLane 1: Rabbit monoclonal IgG(1ug)instead of AAA231837 in Jurkat whole cell lysate. For western blotting, a HRP-conjugated light chain specific antibody was used as the Secondary antibody (1/50000)Lane 2: AAA231837(4ug)+ Jurkat whole cell lysate(500ug)Lane 3: Jurkat whole cell lysate (20ug))
IHC (Immunohiostchemistry)
(Immunohistochemistry of paraffin-embedded human testis tissue using AAA231837 at dilution of 1:100)
IHC (Immunohiostchemistry)
(Immunohistochemistry of paraffin-embedded human testis tissue using AAA231837 at dilution of 1:100)
WB (Western Blot)
(Western blotAll lanes: MFN1 antibody at 1.1 ug/mlLane 1: Human placenta tissueLane 2: Jurkat whole cell lysateLane 3: Hela whole cell lysateLane 4: K562 whole cell lysateSecondaryGoat polyclonal to rabbit IgG at 1/10000 dilutionPredicted band size: 85,42,72 kDaObserved band size: 84,42 kDa)
WB (Western Blot)
(Western blotAll lanes: MFN1 antibody at 1.1 ug/mlLane 1: Human placenta tissueLane 2: Jurkat whole cell lysateLane 3: Hela whole cell lysateLane 4: K562 whole cell lysateSecondaryGoat polyclonal to rabbit IgG at 1/10000 dilutionPredicted band size: 85,42,72 kDaObserved band size: 84,42 kDa)
Related Product Information for anti-MFN1 antibody
Essential transmembrane GTPase, which mediates mitochondrial fusion. Fusion of mitochondria occurs in many cell types and constitutes an important step in mitochondria morphology, which is balanced between fusion and fission. MFN1 acts independently of the cytoskeleton. Overexpression induces the formation of mitochondrial networks.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
71,401 Da
NCBI Official Full Name
mitofusin-1
NCBI Official Synonym Full Names
mitofusin 1
NCBI Official Symbol
MFN1
NCBI Official Synonym Symbols
hfzo1; hfzo2
NCBI Protein Information
mitofusin-1
UniProt Protein Name
Mitofusin-1
UniProt Gene Name
MFN1
UniProt Entry Name
MFN1_HUMAN
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Product Notes
The MFN1 mfn1 (Catalog #AAA231837) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MFN1 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MFN1 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), IHC (Immunohistochemistry), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the MFN1 mfn1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MFN1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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