IHC (Immunohiostchemistry)
(MIA40 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of MIA40 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Rabbit anti-Human MIA40 Polyclonal Antibody | anti-CHCHD4 antibody
MIA40 Antibody (C-term)
Gene Names
CHCHD4; MIA40; TIMM40
Reactivity
Human
Applications
Immunohistochemistry, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
MIA40, Antibody; MIA40 Antibody (C-term); Mitochondrial intermembrane space import and assembly protein 40; Coiled-coil-helix-coiled-coil-helix domain-containing protein 4; CHCHD4; MIA40; anti-CHCHD4 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
This MIA40 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 101-129 amino acids from the C-terminal region of human MIA40.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.45 (varies by lot)
Sequence Positions
101-129
Sequence Length
155
Applicable Applications for anti-CHCHD4 antibody
IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Cellular Location
Mitochondrion intermembrane space
Tissue Location
Expressed in all tissues tested, suggesting an ubiquitous expression.
Preparation and Storage
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
IHC (Immunohiostchemistry)
(MIA40 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of MIA40 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(MIA40 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of MIA40 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot)
(MIA40 Antibody (C-term) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the MIA40 antibody detected the MIA40 protein (arrow).)
WB (Western Blot)
(MIA40 Antibody (C-term) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the MIA40 antibody detected the MIA40 protein (arrow).)
Related Product Information for anti-CHCHD4 antibody
Central component of a redox-sensitive mitochondrial intermembrane space import machinery which is required for the biogenesis of respiratory chain complexes (PubMed:26004228). Functions as chaperone and catalyzes the formation of disulfide bonds in substrate proteins, such as COX17, COX19, MICU1 and COA7 (PubMed:16185709, PubMed:26387864, PubMed:19182799, PubMed:21059946, PubMed:23186364, PubMed:23676665, PubMed:30885959). Required for the import and folding of small cysteine-containing proteins (small Tim) in the mitochondrial intermembrane space (IMS). Required for the import of COA7 in the IMS (PubMed:30885959). Precursor proteins to be imported into the IMS are translocated in their reduced form into the mitochondria. The oxidized form of CHCHD4/MIA40 forms a transient intermolecular disulfide bridge with the reduced precursor protein, resulting in oxidation of the precursor protein that now contains an intramolecular disulfide bond and is able to undergo folding in the IMS (PubMed:16185709, PubMed:19182799, PubMed:21059946, PubMed:23676665). Reduced CHCHD4/MIA40 is then reoxidized by GFER/ERV1 via a disulfide relay system (PubMed:23186364). Mediates formation of disulfide bond in MICU1 in the IMS, promoting formation of the MICU1-MICU2 heterodimer that regulates mitochondrial calcium uptake (PubMed:26387864).
Product Categories/Family for anti-CHCHD4 antibody
References
Daithankar, V.N., et al. Biochemistry 48(22):4828-4837(2009)
Chacinska, A., et al. J. Biol. Chem. 283(44):29723-29729(2008)
Terziyska, N., et al. FEBS Lett. 581(6):1098-1102(2007)
Hofmann, S., et al. J. Mol. Biol. 353(3):517-528(2005)
Chacinska, A., et al. J. Biol. Chem. 283(44):29723-29729(2008)
Terziyska, N., et al. FEBS Lett. 581(6):1098-1102(2007)
Hofmann, S., et al. J. Mol. Biol. 353(3):517-528(2005)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
15996
NCBI Official Full Name
mitochondrial intermembrane space import and assembly protein 40 isoform 1
NCBI Official Synonym Full Names
coiled-coil-helix-coiled-coil-helix domain containing 4
NCBI Official Symbol
CHCHD4
NCBI Official Synonym Symbols
MIA40; TIMM40
NCBI Protein Information
mitochondrial intermembrane space import and assembly protein 40
UniProt Protein Name
Mitochondrial intermembrane space import and assembly protein 40
UniProt Gene Name
CHCHD4
UniProt Synonym Gene Names
MIA40
UniProt Entry Name
MIA40_HUMAN
Similar Products
Product Notes
The CHCHD4 chchd4 (Catalog #AAA288895) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 101-129. The MIA40 Antibody (C-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MIA40 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the CHCHD4 chchd4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MIA40, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.