IP (Immunoprecipitation)
(MICB was immunoprecipitated using:; Lane A:0.5 mg Jurkat Whole Cell Lysate; 4 uL anti-MICB rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.; Primary antibody:; Anti-MICB rabbit polyclonal antibody,at 1:100 dilution; Secondary antibody:; Clean-Blot IP Detection Reagent (HRP) at 1:1000dilution; Developed using the ECL technique.; Performed under reducing conditions.; Predicted band size: 40 kDa; Observed band size :55 kDa)
Rabbit anti-Human MICB Polyclonal Antibody | anti-MICB antibody
MICB Antibody, Rabbit PAb, Antigen Affinity Purified
Reactivity
Human
Applications
Immunoprecipitation, ELISA, Western Blot
Synonyms
MICB, Antibody; MICB Antibody, Rabbit PAb, Antigen Affinity Purified; Anti-PERB11.2 Antibody; anti-MICB antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
Human MICB
Applicable Applications for anti-MICB antibody
IP (Immunoprecipitation), ELISA, WB (Western Blot)
Preparation
Produced in rabbits immunized with purified, recombinant Human MICB (rh MICB;NP_005922.2; Met1-Gly298). MICB specific IgG was purified by Human MICB affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
IP (Immunoprecipitation)
(MICB was immunoprecipitated using:; Lane A:0.5 mg Jurkat Whole Cell Lysate; 4 uL anti-MICB rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.; Primary antibody:; Anti-MICB rabbit polyclonal antibody,at 1:100 dilution; Secondary antibody:; Clean-Blot IP Detection Reagent (HRP) at 1:1000dilution; Developed using the ECL technique.; Performed under reducing conditions.; Predicted band size: 40 kDa; Observed band size :55 kDa)
IP (Immunoprecipitation)
(MICB was immunoprecipitated using:; Lane A:0.5 mg Jurkat Whole Cell Lysate; 4 uL anti-MICB rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.; Primary antibody:; Anti-MICB rabbit polyclonal antibody,at 1:100 dilution; Secondary antibody:; Clean-Blot IP Detection Reagent (HRP) at 1:1000dilution; Developed using the ECL technique.; Performed under reducing conditions.; Predicted band size: 40 kDa; Observed band size :55 kDa)
WB (Western Blot)
(Anti-MICB rabbit polyclonal antibody at 1:500 dilution; Lane A: Jurkat Whole Cell Lysate; Lane B: MCF-7 Whole Cell Lysate; Lane C: HepG2 Whole Cell Lysate; Lane D: Daudi Whole Cell Lysate; Lysates/proteins at 30 ug per lane.; Secondary; Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.; Developed using the ECL technique.; Performed under reducing conditions.; Predicted band size:43 kDa; (We are unsure as to the identity of these extra bands.))
WB (Western Blot)
(Anti-MICB rabbit polyclonal antibody at 1:500 dilution; Lane A: Jurkat Whole Cell Lysate; Lane B: MCF-7 Whole Cell Lysate; Lane C: HepG2 Whole Cell Lysate; Lane D: Daudi Whole Cell Lysate; Lysates/proteins at 30 ug per lane.; Secondary; Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.; Developed using the ECL technique.; Performed under reducing conditions.; Predicted band size:43 kDa; (We are unsure as to the identity of these extra bands.))
Related Product Information for anti-MICB antibody
MHC class I polypeptide-related sequence B, also known as MICB, is a heavily glycosylated protein serving as a ligand for the type II receptor NKG2D. MICB shares 85% amino acid identity with MICA, a closely related protein, both of which contain three extracellular immunoglobulin-like domains, but without the capacity to bind peptide or interact with beta-2-microglobulin. acting as a stress-induced self-antigen, binding of MICB to the NKG2D receptor activates the cytolytic response of natural killer (NK) cells, CD8+alphabeta T cells, and gammadelta T cells on which the receptor is expressed. MICA/B is minimally expressed on normal cells, but are frequently expressed on epithelial tumors and can be induced by bacterial and viral infections. MICA/B recognition thus is involved in tumor surveillance, viral infections, and autoimmune diseases.
Product Categories/Family for anti-MICB antibody
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Product Notes
The MICB (Catalog #AAA258692) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MICB Antibody, Rabbit PAb, Antigen Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MICB can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the MICB for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MICB, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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