WB (Western Blot)
(Detection of human and mouse MIOS by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-MIOS antibody AAA212870 (lot AAA212870-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
Rabbit anti-Human, Mouse MIOS Polyclonal Antibody | anti-MIOS antibody
Rabbit anti-MIOS Antibody, Affinity Purified
Reactivity
Human, Mouse
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
MIOS, Antibody; Rabbit anti-MIOS Antibody, Affinity Purified; missing oocyte, meiosis regulator, homolog; Sea4; Yulink; WUGSC:H_DJ1159O04.1; Missing oocyte meiosis regulator homolog; GATOR complex protein MIOS; MIO; H_DJ1159O04.1; WD repeat-containing protein mio; meiosis regulator for oocyte development; anti-MIOS antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-MIOS antibody
IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Orangutan
Immunogen
Between 825 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human and mouse MIOS by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-MIOS antibody AAA212870 (lot AAA212870-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
WB (Western Blot)
(Detection of human and mouse MIOS by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-MIOS antibody AAA212870 (lot AAA212870-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IP (Immunoprecipitation)
(Detection of human MIOS by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-MIOS antibody AAA212870 (lot AAA212870-1) used for IP at 6 ug per reaction. MIOS was also immunoprecipitated by rabbit anti-MIOS antibody BL18122. For blotting immunoprecipitated MIOS, AAA212870 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human MIOS by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-MIOS antibody AAA212870 (lot AAA212870-1) used for IP at 6 ug per reaction. MIOS was also immunoprecipitated by rabbit anti-MIOS antibody BL18122. For blotting immunoprecipitated MIOS, AAA212870 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Related Product Information for anti-MIOS antibody
MIOS is a component of the GATOR2 complex. It inhibits GATOR1 complex, an inhibitor of the amino acid-sensing branch of the TORC1 pathway [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q9NXC5].
Product Categories/Family for anti-MIOS antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
98,584 Da
NCBI Official Full Name
WD repeat-containing protein mio
NCBI Official Synonym Full Names
missing oocyte, meiosis regulator, homolog (Drosophila)
NCBI Official Symbol
MIOS
NCBI Protein Information
WD repeat-containing protein mio; H_DJ1159O04.1; WUGSC:H_DJ1159O04.1
UniProt Protein Name
WD repeat-containing protein mio
UniProt Gene Name
MIOS
UniProt Entry Name
MIO_HUMAN
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Product Notes
The MIOS mios (Catalog #AAA212870) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-MIOS Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's MIOS can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the MIOS mios for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MIOS, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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