Rabbit Mnk1 Polyclonal Antibody | anti-Mnk1 antibody

Phospho-Mnk1 (Thr250) Antibody

Cat. Num. AAA31406

• Gene Names: MKNK1; MNK1
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Pig (100%), Zebrafish (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%), Xenopus (100%)
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, ELISA
• Purity: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
• Synonyms: Mnk1; Polyclonal Antibody; Phospho-Mnk1 (Thr250) Antibody; MAP kinase interacting kinase 1; MAP kinase interacting serine/threonine kinase 1; MAP kinase signal integrating kinase 1; MAP kinase signal-integrating kinase 1; MAP kinase-interacting serine/threonine-protein kinase 1; MAPK signal integrating kinase 1; MITOGEN-ACTIVATED PROTEIN KINASE-INTERACTING SERINE/THREONINE KINASE 1; mknk1; MKNK1_HUMAN; MNK 1; anti-Mnk1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Pig (100%), Zebrafish (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%), Xenopus (100%)
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Phospho-Mnk1 (Thr250) Antibody detects endogenous levels of Mnk1 only when phosphorylated at Thr250.; Tissue Specificity: Ubiquitous.
• Purity/Purification: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-Mnk1 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Peptide ELISA (EIA)
• Application Notes: WB: 1:1000-3000; IHC: 1:50-1:200; IF/ICC: 1:100-1:500; Peptide ELISA: 1:20,000-1:40,000
• Immunogen: A synthesized peptide derived from human Mnk1 around the phosphorylation site of Thr250.
• Conjugation: Unconjugated
• Fragment: Fab fragment
• Post Translational Modifications: Dual phosphorylation of Thr-250 and Thr-255 activates the kinase. Phosphorylation of Thr-385 activates the kinase. MAPK3/ERK1 is one of the kinases which activate MKNK1/MNK1. Phosphorylation by PAK2 leads to a reduced phosphorylation of EIF4G1.
• Subunit Structure: Interacts with the C-terminal regions of EIF4G1 and EIF4G2. Also binds to dephosphorylated ERK1 and ERK2, and to the p38 kinases.
• Similarity: Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.
• Subcellular Location: Cytoplasm. Cytoplasm. Nucleus.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Application Data

(At 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

IHC (Immunohistochemistry)

(At 1/200 staining Human liver cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry)

(At 1/200 staining Mouse kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry)

(At 1/200 staining Rat kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.)

WB (Western Blot)

(Western blot analysis of MNK1 (Phospho-Thr250) using TNF treated Jurkat whole cell lysates.-/+ means absence or presence of N peptide（non-phospho peptide) and P peptide(phospho peptide).)

WB (Western Blot)

(Western blot analysis of extracts from 293 cells(UV treatment), using Phospho-Mnk1 (Thr250) Antibody. The lane on the left was treated with blocking peptide.)

Related Product Information for anti-Mnk1 antibody

May play a role in the response to environmental stress and cytokines. Appears to regulate translation by phosphorylating EIF4E, thus increasing the affinity of this protein for the 7-methylguanosine-containing mRNA cap.

NCBI and Uniprot Product Information

• NCBI GI #: 207113186
• NCBI GeneID: 8569
• NCBI Accession #: NP_001129025.1
• NCBI GenBank Nucleotide #: NM_001135553.2
• UniProt Accession #: Q9BUB5; O00312; Q5TC06; Q5TC07; Q6V0N6; D3DQ20; D3DQ21
• Molecular Weight: 51,342 Da
• NCBI Official Full Name: MAP kinase-interacting serine/threonine-protein kinase 1 isoform 3
• NCBI Official Synonym Full Names: MAP kinase interacting serine/threonine kinase 1
• NCBI Official Symbol: MKNK1
• NCBI Official Synonym Symbols: MNK1
• NCBI Protein Information: MAP kinase-interacting serine/threonine-protein kinase 1; MAPK signal-integrating kinase 1; MAP kinase signal-integrating kinase 1
• UniProt Protein Name: MAP kinase-interacting serine/threonine-protein kinase 1
• UniProt Gene Name: MKNK1
• UniProt Synonym Gene Names: MNK1; MAPK signal-integrating kinase 1; Mnk1
• UniProt Entry Name: MKNK1_HUMAN